Genetic Variation of <i>Erigeron breviscapus </i>(Vant.) Hand-Mazz in Yunnan under Heavy Metals Stress

Abstract

Erigeron breviscapus (Vant.) Hand-Mazz is the raw materials to product Health drugs and injectable formulations. Study the genetic diversity of Erigeron breviscapus under harmful substances stress provides insight into their genetic variation characteristics under stress. In this research, genetic diversity of Erigeron breviscapus under heavy metals (Cr, Cd, Hg, Cu, Pb) stress with different concentrations were analyzed by using 8 inter-simple sequence repeat markers (ISSR). The results showed that Erigeron breviscapus had rich genetic variation under five different kinds heavy metals stress. A total of 96 bands were amplified, of which 80 polymorphic bands were discovered and the percentage of polymorphic bands (PPB) was 83.33%. Their indexes of Na, Ne, H, I and PPB were calculated. Richer genetic diversity was discovered under Cu stress, followed by Cd, Cr, Pb and Hg stress in order. This study will be provide a scientific basis to improve the quality Erigeron breviscapus and optimize its growing conditions.