Genetic variation in Malaysian oysters: taxonomic ambiguities and evidence of biological invasion

Mustaffa, Suzana; Lutfi, A.M.; Hadi, Asma Abdul; Devakie, M.N.; Azizah, Mohd Nor Siti

doi:10.1007/s10530-011-0009-8

Cited by 15 publications

(4 citation statements)

References 20 publications

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“…The inter-specific variation of 16.2 % in the current study is concordant with the earlier study, but our intra-specific variation was markedly different. The current study recorded intra-specific variation of 0.6 % to 0.9 %, which was consistent with a study on Malaysian oysters (Suzana et al, 2011) and deep-sea clams (Liu and Zhang, 2018), which observed values ranging from 0.1 % to 1.1 % and 0.0 % to 2.66 %, respectively. Intra-specific variation in this present study was also in concordance with a comprehensive study of four marine bivalve genera that recorded an intra-species variation of 0.0 % to 3.2 % (Mikkelsen et al, 2007).…”

Section: Species Identificationsupporting

confidence: 92%

Dna Barcoding of Endangered Giant Clams in Islands Off the East Coast of Peninsular Malaysia

Mamat¹,

Yusuf²,

NOR³

et al. 2021

JSSM

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This study reports on the taxonomic verification and distribution of three presumed morphologically identical Tridacna species (giant clams), namely T. crocea, T. maxima and T. squamosa, from nine localities in islands off Terengganu, Pahang and Johor in the east coast of Peninsular Malaysia. A 467-bp partial sequence of mitochondrial DNA cytochrome c oxidase 1 (MT-CO1), which serves as the DNA barcoding gene, was analysed for species identification of 247 samples. The MT-CO1 gene was successfully used to identify all the giant clam samples to species level based on GenBank BLAST and BOLD databases. Three highly-supported clusters were obtained, which supported the morphological species determination into T. crocea, T. maxima and T. squamosa. However, a few discrepancies were observed, which could be attributed to misidentification of juveniles. T. squamosa and T. crocea were more closely related to each other compared to T. maxima. T. maxima and T. squamosa were found to be ubiquitous in all the islands, while T. crocea was restricted to the southeastern islands of Pahang and Johor. The precise identification of samples through the MT-CO1 gene and information on their distributions are useful in strategising the conservation and management of giant clams in this region.

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Section: Species Identificationsupporting

confidence: 92%

Dna Barcoding of Endangered Giant Clams in Islands Off the East Coast of Peninsular Malaysia

Mamat¹,

Yusuf²,

NOR³

et al. 2021

JSSM

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“…This oyster inhabits mangroves and estuaries in southern Thailand, both the Gulf of Thailand and the Andaman Sea, and Surathanee, Songkhla, Pangnga and Ranong provinces (Klinbunga et al 2005). This species has high market demand in comparision with other oyster species (Suzana et al 2011).…”

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confidence: 99%

Chromosomal Characteristics of the Tropical Oyster, <i>Crassostrea belcheri</i> Sowerby, 1871 (Ostreoida, Ostreidae) by Conventional and Ag-NOR Banding Techniques

et al. 2017

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Chromosome preparation from gill tissue of the tropical oyster (Crassostera belcheri Sowerby, 1871) collected from an oyster farm in Kantang District, Trang Province, southern Thailand was studied by conventional staining and Ag-NOR baining techniques. The result revealed that the diploid chromosome number is 2n=20 and the fundamental number (NF) is 40. The karyotype has revealed 10 large metacentric and 10 medium metacentric chromosomes. One pair of nucleolar organizer regions (NORs) was apparent on the short arm telomeric region of chromosome pair 10. Basic information on the cytogenetics of the C. belcheri may be useful for future studies on interspecific hybridization and polyploidy. The karyotype formula of C. belcheri is as follows: m m 10 10 2 (diploid) 20=L +M n

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“…A median joining haplotype network was generated from the mitochondrial COI sequence data using PopART [69, 70] with the default settings. Sequences of S. echinata from previous studies ( n = 21) [32, 71] were obtained from GenBank and also included in the network analyses. Sampling sites of these sequences include Semporna in Malaysia, Taiwan, and Taketomi Island in Japan (coordinates listed in Fig.…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial and nuclear genetic analyses of the tropical black-lip rock oyster (Saccostrea echinata) reveals population subdivision and informs sustainable aquaculture development

et al. 2019

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Background The black-lip rock oyster (Saccostrea echinata) has considerable potential for aquaculture throughout the tropics. Previous attempts to farm S. echinata failed due to an insufficient supply of wild spat; however, the prospect of hatchery-based aquaculture has stimulated renewed interest, and small-scale farming is underway across northern Australia and in New Caledonia. The absence of knowledge surrounding the population genetic structure of this species has raised concerns about the genetic impacts of this emerging aquaculture industry. This study is the first to examine population genetics of S. echinata and employs both mitochondrial cytochrome c oxidase subunit I gene (COI) and single nucleotide polymorphism (SNP) markers. Results The mitochondrial COI data set included 273 sequences of 594 base pair length, which comprised 74 haplotypes. The SNP data set included 27,887 filtered SNPs for 272 oysters and of these 31 SNPs were identified as candidate adaptive loci. Data from the mitochondrial COI analyses, supports a broad tropical Indo-Pacific distribution of S. echinata, and showed high haplotype and nucleotide diversities (0.887–1.000 and 0.005–0.008, respectively). Mitochondrial COI analyses also revealed a ‘star-like’ haplotype network, and significant and negative neutrality tests (Tajima’s D = − 2.030, Fu’s Fs = − 25.638, P < 0.001) support a recent population expansion after a bottleneck. The SNP analyses showed significant levels of population subdivision and four genetic clusters were identified: (1) the Noumea (New Caledonia) sample location; (2) the Bowen (north Queensland, Australia) sample location, and remaining sample locations in the Northern Territory, Australia (n = 8) were differentiated into two genetic clusters. These occurred at either side of the Wessel Islands and were termed (3) ‘west’ and (4) ‘east’ clusters, and two migrant individuals were detected between them. The SNP data showed a significant positive correlation between genetic and geographic distance (Mantel test, P < 0.001, R2 = 0.798) and supported isolation by distance. Three candidate adaptive SNPs were identified as occurring within known genes and gene ontology was well described for the sex peptide receptor gene. Conclusions Data supports the existence of genetically distinct populations of S. echinata, suggesting that management of wild and farmed stocks should be based upon multiple management units. This research has made information on population genetic structure and connectivity available for a new aquaculture species.

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Genetic variation in Malaysian oysters: taxonomic ambiguities and evidence of biological invasion

Cited by 15 publications

References 20 publications

Dna Barcoding of Endangered Giant Clams in Islands Off the East Coast of Peninsular Malaysia

Dna Barcoding of Endangered Giant Clams in Islands Off the East Coast of Peninsular Malaysia

Chromosomal Characteristics of the Tropical Oyster, <i>Crassostrea belcheri</i> Sowerby, 1871 (Ostreoida, Ostreidae) by Conventional and Ag-NOR Banding Techniques

Mitochondrial and nuclear genetic analyses of the tropical black-lip rock oyster (Saccostrea echinata) reveals population subdivision and informs sustainable aquaculture development

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