Chromosomal Characteristics of the Tropical Oyster, &lt;i&gt;Crassostrea belcheri&lt;/i&gt; Sowerby, 1871 (Ostreoida, Ostreidae) by Conventional and Ag-NOR Banding Techniques

Chooseangjaew, Supatcha; Tanyaros, Suwat; Maneechot, Nuntiya; Buasriyot, Phichaya; Getlekha, Nuntaporn; Tanomtong, Alongklod

doi:10.1508/cytologia.82.3

Cited by 22 publications

(34 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conventional staining was done using 10% Giemsa s solution for 10 min (Chooseangjaew et al 2017). Ag-NOR banding was performed (Howell and Black 1980) by applying two drops of 2% gelatin on the slides, followed with four drops of 50% silver nitrate.…”

Section: Methodsmentioning

confidence: 99%

“…The relative length (RL), the centromeric index (CI), and standard deviation (SD) of RL and CI were estimated (Chaiyasut 1989). The CI (q/p+q) between 0.50-0.59, 0.60-0.69, 0.70-0.89, and 0.90-0.99 were described as metacentric, submetacentric, acrocentric and telocentric chromosomes, respectively (Chooseangjaew et al 2017). All data were used in karyotyping and idiograming.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Cytogenetic Study of Three Microhylid Species (Anura, Microhylidae) from Thailand

et al. 2017

Self Cite

View full text Add to dashboard Cite

The cytogenetics of the ornamented pygmy frog (Microhyla fissipes), painted chorus frog (M. pulchra) and narrow-mouthed frog (M. heymonsi) were studied in the aspect of chromosome numbers, morphology and nucleolus organizer region (NOR) locations. For this present study, we provided the karyotype and idiogram of these three species by conventional and Ag-NOR staining techniques. The 10 male and female samples of those three species were collected in Northeast Thailand. The metaphase chromosome preparations were prepared from the bone marrows by the standard protocol. To stain the chromosomes, 10% Giemsa s and 50% silver nitrate were applied. The results showed that all three species had the same diploid chromosome number of 2n=24, and fundamental numbers (NF) were 48 in both males and females. The chromosomes of M. fissipes were comprised of 16m+6sm+XY (X and Y were metacentric chromosomes). M. pulchra had chromosomes consisting of 12m+12sm and M. heymonsi had chromosomes comprising of 18m+6 with no obvious difference in size of sex chromosomes for the two species. M. fissipes and M. pulchra had the same NORs adjacent to the subcentromeric on the long arm of chromosome pair 9, while M. heymonsi had the NOR location on the short arm of chromosome pair 2 close to the centromere. For this case, we suggested that chromosome morphology and the NOR location is still a useful cytological character for taxonomic and evolutionary studies.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Cytogenetic Study of Three Microhylid Species (Anura, Microhylidae) from Thailand

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Mitotic chromosome preparations were made from anterior kidney after in vivo treatment using a method modified from Chen and Ebeling (1968), Gold et al (1990), Kasiroek et al (2017) and Supiwong et al (2017). The chromosomes were stained with 10% Giemsa s for 30 min (Chooseangjaew et al 2017) and identified for NORs by Ag-NOR staining technique (Howell andBlack 1980, Sangpakdee et al 2017). The length of short arm (Ls) and long arm (Ll) chromosomes were measured and the length of total arm chromosome was calculated (LT, LT=Ls+Ll).…”

Section: Methodsmentioning

confidence: 99%

The First Chromosomal Characteristics of Nucleolar Organizer Regions and Karyological Analysis of Two Chaetodontid Fishes (Perciformes, Chaetodontidae)

et al. 2017

Self Cite

View full text Add to dashboard Cite

The first chromosomal characteristics of nucleolar organizer regions (NORs) and karyological analysis of triangle butterflyfish (Chaetodon triangulum) and yellow butterflyfish (C. andamanensis) from Andaman Sea, Phuket and Phang Nga Province, Thailand, were studied. Kidney cell samples were taken from five male and five female fish. Mitotic chromosome preparations were conducted using standard squash technique as well as taken directly from kidney cells. Metaphase spreads were performed on microscopic slides and then airdried. Conventional and Ag-NOR banding techniques were applied to stain the chromosome. The results showed that the diploid chromosome number of C. triangulum and C. andamanensis was 2n=48, and the fundamental numbers (NFs) were 48 and 52, respectively. Karyotpes were present as 46 large telocentric and two medium telocentric chromosomes in C. triangulum; and 2 large metacentric, 8 large telocentric, 34 medium telocentric, 2 medium acrocentric, and 2 small telocentric chromosomes in C. andamanensis. No strange sized chromosomes related to sex were observed. After the Ag-NOR banding technique, one pair of NORs was observed on the long arm centromeric region of large telocentric chromosome pair 21 in C. triangulum and on the long arm subcentromeric region of the largest metacentric chromosome pair 1 in C. andamanensis. The karyotype formulas are as follows:

show abstract

“…Preparation of their chromosome was conducted by squash technique from bone marrow and testis (Kasiroek et al 2017). The chromosomes were stained with 10% Giemsa s for 30 min (Chooseangjaew et al 2017) and identified for NORs by Ag-NOR staining (Howell andBlack 1980, Sangpakdee et al 2017). The length of short arm (Ls) and long arm (Ll) chromosome were measured and calculated for the length of total arm chromosome (LT, LT=Ls+Ll).…”

Section: Methodsmentioning

confidence: 99%

“…Relative length (RL) and centromeric index (CI) were also calculated. CI was estimated to classify the types of chromosomes according to Chaiyasut (1989) and Chooseangjaew et al (2017). All parameters were used in karyotyping and idiograming.…”

Section: Methodsmentioning

confidence: 99%

Cytogenetic of Skink (Reptilia, Scincidae) from Thailand: II: Chromosome Analyses of Stripe Tree Skink (<i>Lipinia vittigera</i>)

et al. 2017

Self Cite

View full text Add to dashboard Cite

Chromosomal analyses of the stripe tree skink (Lipinia vittigera Boulenger, 1894) from mitotic and meiotic cell divisions were studied. Bone marrow and testis samples were taken from five male and five female skinks. Skink chromosome preparations were conducted by squash technique from bone marrow and testis. The chromosomes were stained by conventional staining and Ag-NOR banding techniques. The results showed that the diploid chromosome number of L. vittigera was 2n=28, and the fundamental number (NF) was 44 in both males and females. The types of chromosomes were present as 8 large metacentric, 2 medium metacentric, 6 small metacentric macrochromosomes, and 12 microchromosomes. There was no observation of strange size chromosomes related to sex. Nucleolar organizer regions (NORs) appear to the telomere on the long arm of the largest metacentric chromosome pair 1 and medium metacentric chromosome pair 5. We found that during metaphase I on meiosis, the homologous chromosomes showed synapsis, which can be defined as the 14 bivalents and 14 haploid chromosomes at metaphase II as diploid species. The karyotype formula of L. vittigera is as follows: m m m 8 2 6 2 (diploid) 28=L +M +S +12 microchromosomes n

show abstract

Chromosomal Characteristics of the Tropical Oyster, <i>Crassostrea belcheri</i> Sowerby, 1871 (Ostreoida, Ostreidae) by Conventional and Ag-NOR Banding Techniques

Cited by 22 publications

References 20 publications

Cytogenetic Study of Three Microhylid Species (Anura, Microhylidae) from Thailand

Cytogenetic Study of Three Microhylid Species (Anura, Microhylidae) from Thailand

The First Chromosomal Characteristics of Nucleolar Organizer Regions and Karyological Analysis of Two Chaetodontid Fishes (Perciformes, Chaetodontidae)

Cytogenetic of Skink (Reptilia, Scincidae) from Thailand: II: Chromosome Analyses of Stripe Tree Skink (<i>Lipinia vittigera</i>)

Contact Info

Product

Resources

About