Genetic Variation in <i>Fusarium moniliforme</i> Isolated from Seeds of Different Host Species from Burkina Faso Based on Random Amplified Polymorphic DNA Analysis

Kini, K. Ramachandra; Leth, Vibeke; Mathur, Shivangi

doi:10.1046/j.1439-0434.2002.00739.x

Cited by 27 publications

(12 citation statements)

References 12 publications

(12 reference statements)

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“…Bakanae is an important fungal disease of rice posing threat to rice production all over the world. The disease incidence of bakanae on rice has increased at an alarming rate taking its toll in diverse rice growing regions such as India, Japan, Taiwan, and Thailand ( Kini et al, 2002 ; Saremi, 2005 ; Bashyal and Aggarwal, 2013 ; Gupta et al, 2015 ; Bashyal et al, 2016a ; Fiyaz et al, 2016 ). Apart from causing significant yield losses ( Ou, 1985 ), the bakanae pathogen also affects seed quality ( Bashyal et al, 2014 , 2016b ; Fiyaz et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Whole Genome Sequencing of Fusarium fujikuroi Provides Insight into the Role of Secretory Proteins and Cell Wall Degrading Enzymes in Causing Bakanae Disease of Rice

et al. 2017

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Fusarium fujikuroi causing bakanae disease has emerged as one of the major pathogen of rice across the world. The study aims to comparative genomic analysis of Fusarium fujikuroi isolates and identification of the secretary proteins of the fungus involved in rice pathogenesis. In the present study, F. fujikuroi isolate “F250” was sequenced with an assembly size of 42.47 Mb providing coverage of 96.89% on reference IMI58289 genome. A total of 13,603 protein-coding genes were predicted from genome assembly. The average gene density in the F. fujikuroi genome was 315.10 genes per Mb with an average gene length of 1.67 kb. Additionally, 134,374 single nucleotide polymorphisms (SNPs) are identified against IMI58289 isolate, with an average SNP density of 3.11 per kb of genome. Repetitive elements represent approximately 270,550 bp, which is 0.63% of the total genome. In total, 3,109 simple sequence repeats (SSRs), including 302 compound SSRs are identified in the 8,656 scaffolds. Comparative analysis of the isolates of F. fujikuroi revealed that they shared a total of 12,240 common clusters with F250 showing higher similarity with IMI58289. A total of 1,194 secretory proteins were identified in its genome among which there were 356 genes encoding carbohydrate active enzymes (CAZymes) capable for degradation of complex polysaccharides. Out of them glycoside hydrolase (GH) families were most prevalent (41%) followed by carbohydrate esterase (CE). Out of them CE8 (4 genes), PL1 (10 genes), PL3 (5 genes), and GH28 (8 genes) were prominent plant cell wall degrading enzymes families in F250 secretome. Besides this, 585 genes essential for the pathogen–host interactions were also identified. Selected genes were validated through quantitative real-time PCR analyses in resistant and susceptible genotypes of rice at different days of inoculation. The data offers a better understanding of F. fujikuroi genome and will help us enhance our knowledge on Fusarium fujikuroi–rice interactions.

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Section: Introductionmentioning

confidence: 99%

Whole Genome Sequencing of Fusarium fujikuroi Provides Insight into the Role of Secretory Proteins and Cell Wall Degrading Enzymes in Causing Bakanae Disease of Rice

et al. 2017

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“…Currently, there is very limited information on occurrence and population structure of G. fujikuroi species complex associated with rice. Some data are available on the characterization of G. fujikuroi species complex populations associated with rice seeds from Nepal (Desjardins et al, 2000), few strains were reported associated with rice seeds from Burkina Faso (Kini et al, 2002) and with rice seedlings from Ghana (Amoah et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Fusarium spp. associated with rice Bakanae: ecology, genetic diversity, pathogenicity and toxigenicity

Wulff

Sørensen

Lübeck

et al. 2010

Environmental Microbiology

168

125

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African and Asian populations of Fusarium spp. (Gibberella fujikuroi species complex) associated with Bakanae of rice (Oryzae sativa L.) were isolated from seeds and characterized with respect to ecology, phylogenetics, pathogenicity and mycotoxin production. Independent of the origin, Fusarium spp. were detected in the different rice seed samples with infection rate ranges that varied from 0.25% to 9%. Four Fusaria (F. andiyazi, F. fujikuroi, F. proliferatum and F. verticillioides) were found associated with Bakanae of rice. While three of the Fusaria were found in both African and Asian seed samples, F. fujikuroi was only detected in seed samples from Asia. Phylogenetic studies showed a broad genetic variation among the strains that were distributed into four different genetic clades. Pathogenicity tests showed that all strains reduced seed germination and possessed varying ability to cause symptoms of Bakanae on rice, some species (i.e. F. fujikuroi) being more pathogenic than others. The ability to produce fumonisins (FB(1) and FB(2)) and gibberellin A3 in vitro also differed according to the Fusarium species. While fumonisins were produced by most of the strains of F. verticillioides and F. proliferatum, gibberellin A3 was only produced by F. fujikuroi. Neither fumonisin nor gibberellin was synthesized by most of the strains of F. andiyazi. These findings provide new information on the variation within the G. fujikuroi species complex associated with rice seed and Bakanae disease.

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“…The random amplified polymorphic DNA (deoxyribonucleic acid) or RAPD technique is an alternative strategy for generating a polymorphic class of molecular markers (Williams et al, 1990), without any prior knowledge of DNA sequences in an organism. In Fusarium sp., RAPD has been applied in different approaches, such as to investigate the association between the genetic variability of the isolates and the locality of isolation and/or host plant populations (Kini et al 2002;Pamphile and Azevedo, 2002), to elucidate the taxonomic status and relationships among Fusarium isolates, races and species (Miedaner et al, 2001), to investigate pathogenicity variation (Nelson et al, 1997;Carter et al, 2002;Cramer et al, 2003). In addition, RAPD assay has been used to study the relationship between mycotoxin production in different fungal species and the RAPD profiles; however no conclusive data have been found to date (Jiménez et al, 2000;TranDinh et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Fusarium verticillioides strains isolated from corn feed: characterization by fumonisin production and RAPD fingerprinting

Ono

Fungaro²,

Sofia³

et al. 2010

Braz. arch. biol. technol.

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Genetic Variation in Fusarium moniliforme Isolated from Seeds of Different Host Species from Burkina Faso Based on Random Amplified Polymorphic DNA Analysis

Cited by 27 publications

References 12 publications

Whole Genome Sequencing of Fusarium fujikuroi Provides Insight into the Role of Secretory Proteins and Cell Wall Degrading Enzymes in Causing Bakanae Disease of Rice

Whole Genome Sequencing of Fusarium fujikuroi Provides Insight into the Role of Secretory Proteins and Cell Wall Degrading Enzymes in Causing Bakanae Disease of Rice

Fusarium spp. associated with rice Bakanae: ecology, genetic diversity, pathogenicity and toxigenicity

Fusarium verticillioides strains isolated from corn feed: characterization by fumonisin production and RAPD fingerprinting

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