Genetic variation and taxonomy of Rasbora group (Cyprinidae) from Lake Laut Tawar, Indonesia

Abstract

There were three presumed form of Rasbora found in Lake Laut Tawar, i.e. Depik, Eas and Relo. However, their phylogenetic status was remaining unclear. For this purposes the genetic data were utilized to resolve the taxonomic ambiguity of Rasbora group in Lake Laut Tawar. Approximately 655 bp were amplified from the 5' region of the mitochondrial cytochrome oxidase subunit I (cox1) gene using the primer pairs (Fish F1 and Fish R1). A total of 14 haplotype sequences were produced from the ingroup where haplotypes 1 to 8 were shared by Eas and Depik, and haplotypes 9 to 14 were belonging to Relo. The genetic divergence varied from 0.2 to 9.6% i.e. between Eas and Relo, 9.6% and between Depik and Relo, 9.5%. The genetic data strongly indicated that Eas and Depik should be regarded as the same species of Rasbora tawarensis.

Morphometric Variations of Rasbora Group (Pisces: Cyprinidae) in Lake Laut Tawar, Aceh Province, Indonesia, Based on Truss Character Analysis

“…It was confirmed that Eas and Depik belong to the same species, R. tawarensis, and Relo was a distinct species, by a lower extent morphometric data from present study which was supported by the genetic data from previous report of Muchlisin et al (2012).…”

“…Based on previous report by Muchlisin et al (2012), Eas and Depik have a very close relation genetically. A similar result was demonstrated from morphometric data in the present study, indicating that the morphometric data is in agreement with genetic data from previous study.…”

“…the small one as Relo, medium one as Depik, and the largest one as Eas. However, clarification has been made by Muchlisin et al (2012), by using further study on genetic variation of Rasbora group in this lake, that proposed Eas and Depik as a same species Rasbora tawarensi, while Relo was a different species.…”

“…Information on genetic data of Rasbora group in Lake Laut Tawar has been reported by Muchlisin et al (2012), however no morphometric data was available hitherto. Hence, the objectives of the present study was to evaluate the morphometric variations among three presumed Rasbora taxa (locally known as Eas, Depik and Relo) based on their truss morphometric character, in order to contribute important information on the Rasbora group in Lake Laut Tawar, Aceh Province, Indonesia.…”

DNA Barcoding of Freshwater Fishes from Lake Laut Tawar, Aceh Province, Indonesia

“…Genetic study on C. carpio from Turkey and Uzbekistan were reported by some researchers (Murakaeva et al 2003, Kohlmann et al 2005, Memiş and Kohlmann 2006, and X. hellerii was reported by Mejía et al (2012), however, the genetic characteristic of wild C. carpio, X. hellerii, C. gariepinus, and O. niloticus from Lake Laut Tawar, Indonesia have not previously been evaluated. This study was the first report on the genetic status of freshwater fishes in Aceh region Indonesia, except for Rasbora group in Lake Laut Tawar which was reported by Muchlisin et al (2012).…”