Genetic variation and population structure of swimming crab (Portunus trituberculatus) inferred from mitochondrial control region

Abstract

Genetic variation and population structure in Portunus trituberculatus along the coast of China were revealed according to 617 bp of mitochondrial DNA control region. 90 polymorphic sites defined 53 distinct haplotypes, showing a moderately high diversity among 72 individuals sampled from eight localities. Neighbor-joining tree, statistics analyses of gene flow and genetic differentiation index indicated two populations from Beihai and Laizhou had differentiated. The population from Yingkou, Dandong, Laizhou and Beihai had smaller genetic diversity compared to that from Ningbo, Lianyungang, Qingdao and Japan according to the genetic distance. And mantel test showed significant positive correlation between genetic distance and geographic distance for P. trituberculatus. TCS parsimony network suggested that all the animals sampled were probably the result of recent divergence from a common ancestral haplotype but for Laizhou population. Moreover, the haplotype distribution appeared to correlate with a recent colonization followed by localized genetic differentiation. Mismatch distribution results suggested that Ningbo, Yingkou, Qingdao, Lianyungang and Japan populations, particularly Dandong population had experienced a sudden demographic or spatial expansion. The Pleistocene glaciations might contribute to this process.

Phylogeography of the Rock Shell Thais clavigera (Mollusca): Evidence for Long-Distance Dispersal in the Northwestern Pacific

“…In the NW Pacific, Ni et al . [ 7 ] also estimated a period of 120–140 ka that corresponded with dramatic population expansion of various species, including molluscs [ 13 , 64 ], fishes [ 65 , 66 ], and crustaceans [ 67 , 68 ]. These earlier reports indicate the importance of pre-LGM events in determining the demography of marine populations and should be considered in the future.…”

Transcriptome Analysis of the Portunus trituberculatus: De Novo Assembly, Growth-Related Gene Identification and Marker Discovery

“…Therefore, more genome-wide or transcriptome-wide datasets should be generated as a basis for functional genomics approaches aimed at improving the aquaculture performance of this species. Despite the aquacultural and biological importance of Portunus trituberculatus , previous studies have mostly focused on the isolation of microsatellites [6]–[8], the investigation of genetic diversity [9], Sanger-based sequencing of expressed sequence tags (ESTs) [10], [11], the characterization of single functional genes [12]–[14] and sequencing of the mitochondrial genome of Portunus trituberculatus [15]–[17].…”

Control region sequences indicate that multiple externae represent multiple infections by Sacculina carcini (Cirripedia: Rhizocephala)

“…This marker has been widely utilized in vertebrates and insects but also been applied to studies of genetic variability and population structure in a number of commercially important marine crustaceans, for example, the swimming crab Portunus trituberculatus (Guo et al. ), the scalloped lobster Panulirus homarus (Farhadi et al. ), and the mantis shrimp Oratosquilla oratoria (Lui et al.…”

“…The CR exhibits an evolutionary rate threefold to fivefold higher than other regions of the mt genome (Brown et al 1993), making it a popular marker for genetic studies involving a wide range of taxa. This marker has been widely utilized in vertebrates and insects but also been applied to studies of genetic variability and population structure in a number of commercially important marine crustaceans, for example, the swimming crab Portunus trituberculatus (Guo et al 2012), the scalloped lobster Panulirus homarus (Farhadi et al 2013), and the mantis shrimp Oratosquilla oratoria (Lui et al 2010).…”