Genetic variant of <i>SPARC</i> gene and its association with growth traits in Chinese cattle

Abstract

SPARC is a cysteine-rich acidic secreted protein. It is a non-collagen component of bone, which is widely distributed in humans and animals and plays an important role. SPARC has been found in a variety of human cancers (breast cancer, stomach cancer, ovarian cancer, etc.) and diabetes-related research. Especially the muscle and fat metabolism are closely related. In this study, we used a DNA pool to detect a new SNP site (g.12454T > C). A total of 616 samples of four breeds of Qinchuan cattle (QC, n = 176), Xianan cattle (XN, n = 160), Pinan cattle (PN, n = 136) and Jiaxian cattle (JX, n = 144) were analyzed and identified with ARMS-PCR. In addition, we correlated SNP with growth traits and showed significant correlation with growth traits such as rump length, hip width, and body length (p < 0.05). Moreover, we tested the SPARC gene expression level in different tissues belonging to XN adult cattle (n = 3) and found its high expression in muscle tissues (relative to the kidney). Further, we found the SNP is able to increase the SPARC expression level in skeletal muscle (n = 12). According to statistical data, this SNP site may be applied to a molecular marker of an early marker-assisted selection for early growth of beef cattle.Published by Copernicus Publications on behalf of the Leibniz Institute for Farm Animal Biology (FBN).

KAT2B Gene Polymorphisms Are Associated with Body Measure Traits in Four Chinese Cattle Breeds

“…Using the SYBR Green series (Takara), real-time quantitative PCR (qRT-PCR) was performed to detect the gene expression. β-actin was used as a reference gene for normalization [ 19 ]. The primer P ( Table 1 ) for qRT-PCR was designed based on the KAT2B gene mRNA sequence (XM_019966941.1) with Primer 5.0 software (Sangon Biotech (Shanghai) Co., Ltd., China).…”

“…To explore the allele variation of the bovine KAT2B gene, a total of 17 primer pairs were designed to cover the exons and the adjacent introns ( Table S1 , GenBank accession number: AC_000158.1). Forty DNA samples were randomly selected from each cattle breed, to construct for DNA pools of four breeds [ 19 ]. PCR reactions were carried out using a Touchdown PCR System Thermal Cycler Dice (TaKaRa, Dalian, China) and a PCR protocol which was described in a previous study [ 20 ].…”

SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

“…A recent report showed the genetic variant of SPARC gene was associated with growth traits in Chinese cattle. The SPARC gene was highly expressed in skeletal muscle tissue and a SNP site of loci in SPARC gene is significantly associated with cattle body [24]. It implied that SPARC may play important roles in bovine muscle development.…”

Relationships between the Mini-InDel Variants within the Goat CFAP43 Gene and Body Traits

“…Ear tissues from 1827 adult female goats were collected for genomic DNA extraction. Genomic DNA was isolated from the ear tissue using a standard protocol [ 23 , 24 ]. Genomic DNA quality was measured using a Nanodrop 2000 spectrometer (Thermo Scientific, Waltham, MA, USA) and diluted to a working concentration (20 ng/μL) for the detection of genetic variants.…”

“…DNA was extracted using a high-salt extraction method [ 25 , 26 ]. In addition, pre-experiments were carried out, and the pooled DNA samples from each of the 30 goats were tested using polymerase chain reaction (PCR) to analyze the polymorphism of loci [ 23 , 24 ].…”