Genetic variability of the S segment of Toscana virus

Baggieri, Melissa; Marchi, Antonella; Bucci, Paola; Nicoletti, Loredana; Magurano, Fabio

doi:10.1016/j.virusres.2015.01.013

Cited by 9 publications

(7 citation statements)

References 38 publications

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“…Previous analyses of complete/partial S segment sequences from Italy and Spain, with the predominance of one particular virus genotype, revealed limited variability in nucleotide and amino acid sequences (Collao et al 2009, Baggieri et al 2015. The findings in this study reveal that the genetic variation is more pronounced in TOSV strains identified in Anatolia.…”

Section: Toscana Virus In Dogs and S Sequences 615mentioning

confidence: 42%

Canine Infections and Partial S Segment Sequence Analysis of Toscana Virus in Turkey

Dinçer

Karapınar

Öktem

et al. 2016

Vector-Borne and Zoonotic Diseases

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Introduction: Toscana virus (TOSV) is a sandfly-borne bunyavirus with a significant public health impact. Preliminary studies have revealed TOSV exposure in dogs and they were suggested as potential reservoirs. This study was performed to characterize canine TOSV infections in an endemic region. Sequencing of TOSV small (S) segment in several previously identified specimens was also undertaken to reveal viral genealogy. Materials and Methods: Canine and feline plasma were collected in several districts of Mersin province, Mediterranean Anatolia, Turkey, during May-September, 2015. Phlebovirus RNA was screened through two nested polymerase chain reaction (PCR) assays, targeting S and large (L) segments of the viral genome. A kinetoplast minicircle nested PCR was employed for Leishmania DNA detection and typing. Previously collected TOSV-positive specimens from humans, dogs, cats, and sandflies from various regions in Turkey and Cyprus were further evaluated through the S segment PCR. All amplicons were characterized through sequencing. Results: A total of 210 specimens that comprise canine (76.2%) and feline (23.8%) plasma were screened. In three (1.9%) and two (1.3%) canine specimens, TOSV and Leishmania nucleic acids were detected, respectively. The TOSV strains were characterized as genotype B, and Leishmania infantum was identified in positive specimens. Twenty-four partial S segment sequences were amplified, which demonstrated a maximum intramural diversity of 3.88% in the nucleotide level. Sequence comparisons revealed significant similarities to particular genotype B strains characterized in Spain and France, whereas a notable divergence was observed among several TOSV strains. Single or recurrent amino acid substitutions were noted in eight residues of the viral nucleocapsid. Discussion: Canine infections of TOSV genotype B, with temporal and spatial association with L. infantum, were detected. Divergent TOSV S segment sequences with amino acid substitutions, presumably associated with host adaptation, were observed.

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Section: Toscana Virus In Dogs and S Sequences 615mentioning

confidence: 42%

Canine Infections and Partial S Segment Sequence Analysis of Toscana Virus in Turkey

Dinçer

Karapınar

Öktem

et al. 2016

Vector-Borne and Zoonotic Diseases

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“…In two of the cases, a virus of genotype TOSV lineage A was detected (GU270841) [12] or suggested [3], while in one case the lineage remained unknown [11]. TOSV strains are subdivided into three lineages that are more or less congruent with geography [13, 14]. In Italy, lineage A is predominant, however, co-circulation of two genotypes has been shown previously in southeastern France close to the origin of the isolate in this study [15].…”

Section: Discussionmentioning

confidence: 99%

Meningitis and epididymitis caused by Toscana virus infection imported to Switzerland diagnosed by metagenomic sequencing: a case report

et al. 2019

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Background We report a rare case of Toscana virus infection imported into Switzerland in a 23-year old man who travelled to Imperia (Italy) 10 days before onset of symptoms. Symptoms included both meningitis and as well epididymitis. This is only the fourth case of Toscana virus reported in Switzerland. Case presentation The patient presented with lymphocytic meningitis and scrotal pain due to epididymitis. Meningitis was initially treated with ceftriaxone. Herpes simplex, tick-borne encephalitis, enterovirus, measles, mumps, rubella and Treponema pallidum were excluded with specific polymerase chain reaction (PCR) or serology. In support of routine diagnostic PCR and serology assays, unbiased viral metagenomic sequencing was performed of cerebrospinal fluid and serum. Toscana virus infection was identified in cerebrospinal fluid and the full coding sequence could be obtained. Specific PCR in cerebrospinal fluid and blood and serology with Immunoglobulin (Ig) M and IgG against Toscana virus confirmed our diagnosis. Neurological symptoms recovered spontaneously after 5 days. Conclusions This case of Toscana virus infection highlights the benefits of unbiased metagenomic sequencing to support routine diagnostics in rare or unexpected viral infections. With increasing travel histories of patients, physicians should be aware of imported Toscana virus as the agent for viral meningitis and meningoencephalitis. Electronic supplementary material The online version of this article (10.1186/s12879-019-4231-9) contains supplementary material, which is available to authorized users.

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“…The knowledge about the molecular epidemiology of TOSV has largely increased during the last decade. Three TOSV genetic lineages are currently identified, A, B, and C. TOSV lineage A strains were identified in several Mediterranean countries, including Italy, France, and Turkey; TOSV lineage B strains were detected in the Iberian Peninsula and France, while a novel lineage, TOSV lineage C, has been identified in meningitis and severe encephalitis cases in Croatia and Greece, respectively …”

Section: Sandfly‐borne Viral Diseasesmentioning

confidence: 99%

Emerging arboviral human diseases in Southern Europe

Papa

2017

Journal of Medical Virology

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Southern Europe is characterized by unique landscape and climate which attract tourists, but also arthropod vectors, some of them carrying pathogens. Among several arboviral diseases that emerged in the region during the last decade, West Nile fever accounted for high number of human cases and fatalities, while Crimean-Congo hemorrhagic fever expanded its geographic distribution, and is considered as a real threat for Europe. Viruses evolve rapidly and acquire mutations making themselves stronger and naive populations more vulnerable. In an effort to tackle efficiently the emerging arboviral diseases, preparedness and strategic surveillance are needed for the early detection of the pathogen and containment and mitigation of probable outbreaks. In this review, the main human arboviral diseases that emerged in Southern Europe are described.

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Genetic variability of the S segment of Toscana virus

Cited by 9 publications

References 38 publications

Canine Infections and Partial S Segment Sequence Analysis of Toscana Virus in Turkey

Canine Infections and Partial S Segment Sequence Analysis of Toscana Virus in Turkey

Meningitis and epididymitis caused by Toscana virus infection imported to Switzerland diagnosed by metagenomic sequencing: a case report

Emerging arboviral human diseases in Southern Europe

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