Genetic variability of Plum pox virus isolates in the  Czech Republic

Gadiou, S.; Šafářová, Dana; Navrátil, Milan

doi:10.1007/s10658-008-9272-7

Cited by 14 publications

(10 citation statements)

References 16 publications

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“…There are several global studies of the population structures of RNA viruses of human and other animals, but few of those of plants [36,37]. Several papers have been published reporting sequence analyses of regional populations of PPV [38][39][40][41], allowing the local spread and evolution of particular strains to be identified [42][43][44]. Despite these efforts, the global structure of the PPV population has not yet been determined, although it is now possible, as currently (January 2019) there are more than 200 complete genomic sequences of various isolates of PPV available in the GenBank database (https://www.…”

Section: Introductionmentioning

confidence: 99%

The global phylogeny of Plum pox virus is emerging

Hajizadeh

Gibbs

Amirnia

et al. 2019

Journal of General Virology

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The 206 complete genomic sequences of Plum pox virus in GenBank (January 2019) were downloaded. Their main open reading frames (ORF)s were compared by phylogenetic and population genetic methods. All fell into the nine previously recognized strain clusters; the PPV-Rec and PPV-T strain ORFs were all recombinants, whereas most of those in the PPV-C, PPV-CR, PPV-CV, PPV-D, PPV-EA, PPV-M and PPV-W strain clusters were not. The strain clusters ranged in size from 2 (PPV-CV and PPV-EA) to 74 (PPV-D). The isolates of eight of the nine strains came solely from Europe and the Levant (with an exception resulting from a quarantine breach), but many PPV-D strain isolates also came from east and south Asia and the Americas. The estimated time to the most recent common ancestor (TMRCA) of all 134 non-recombinant ORFs was 820 (865-775) BCE. Most strain populations were only a few decades old, and had small intra-strain, but large inter-strain, differences; strain PPV-W was the oldest. Eurasia is clearly the 'centre of emergence' of PPV and the several PPV-D strain populations found elsewhere only show evidence of gene flow with Europe, so have come from separate introductions from Europe. All ORFs and their individual genes show evidence of strong negative selection, except the positively selected pipo gene of the recently migrant populations. The possible ancient origins of PPV are discussed.

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Section: Introductionmentioning

confidence: 99%

The global phylogeny of Plum pox virus is emerging

Hajizadeh

Gibbs

Amirnia

et al. 2019

Journal of General Virology

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“…Molecular variability of some Czech Plum pox virus isolates was studied by Navrátil et al (1998), but in this paper it was not described how and where the isolates were sampled; it was only reported that some of them originated from our institute (RICP Prague), only one case of mixed infection was found, and PPV-D isolates prevailed. Recently, Gadiou et al (2008) studied 24 Czech PPV isolates from five different plum orchards situated in Moravia. PPV-D isolates were identified in all orchards, PPV-Rec only in two of them.…”

Section: Resultsmentioning

confidence: 99%

Distribution of Plum pox virus strains in natural sources in the Czech Republic

Polák¹,

Komínek²

2009

Plant Prot. Sci.

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“…The sequencing BYMV was started from the primers which were covering mainly CP-NIb gene (Oertel et al, 1997;Lecoq et al, 2000). Sequencing of CP and NIb genes is often used for phylogenetic analyses of many species of the genus Potyvirus (Handley et al, 1998;Alegria et al, 2003;Gadiou et al, 2008;Wylie and Jones, 2009;Campos et al, 2014). Several techniques for detecting BYMV are now available ELISA, dot-blot hybridization, sequencing and PCR seemed to be the most promising technique for detection of low amounts infected plants of BYMV (Scott et al, 1989;Efaisha, 2005;Ganesh et al, 2009a;Choi et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Nucleotide Sequence of Capsid Protein Gene of Bean yellow mosaic potyvirus in Bean Plants from Al-Makhwah Governorate, Saudi Arabia

Farag¹,

Khattab²,

Al-sham³

2015

International J. of Virology

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The current study represents the first identification of Bean Yellow Mosaic Virus (BYMV) in bean plants (Phaseolus vulgaris) from Al-Makhwah Governorate, Saudi Arabia and nucleotide sequencing of capsid protein gene of BYMV. Thirty plant species and cultivars to twelve different families were mechanically inoculated by BYMV. Seventeen of them showed systemic symptoms mosaic, yellowing, vein clearing, stunting streaking mosaic, malformation and severe mosaic as a result of BYMV infection. Chenopodium amaranticolor and Chinopodium quinoa L. were found to be local lesion host after 4-6 days of inoculation. Two aphids, Myzus persicae Sluz. and Aphis faba were used to study the transmission of BYMV. Aphis faba was found to be the most effective vector with 60% of BYMV transmission. Immunological techniques namely Enzyme Linked Immuno Sorbent Assay (ELISA), Tissue Blot Immuno Binding Assay (TBIA) and Dot Blot Immune Binding Assay (DBIA) were amplified to study BYMV. Positive reaction was obtained and the prevalence of the virus in the flowers and seed parts was confirmed. The RT-PCR products were amplified from total RNA of bean plant tissues using specific primer BYMV1 and BYMV2 (designed on conserved sequences of BYMV NIb-CP). A cDNA fragment of 700 bp nuclear inclusion body and coat protein gene region primer (5'-NIb-CP 3') was amplified. Digoxigenin-labelled BYMV cDNA probe through Southern blot and Dot blot hybridization techniques were employed for the detection of BYMV infected bean plants. A strong positive reaction was observed with bean and faba bean infected with BYMV. A part of the 3' end of Nib-region and the 5' end of the CP region of BYMV Potyvirus Al-Makhwah KSA isolate (700 nt) was sequenced and analyzed (The DNA sequence submitted in GenBank acc.no. LC025531). Identity percentage of Al-Makhwah KSA isolate BYMV Potyvirus (NIb-CP) with a Japanese isolate was 99% and with USA gladiolus isolate and another Japanese isolate were 95% and 93%, respectively confirming that BYMV viral group is diversified mainly in some specific parts of genome, especially in the CP region, whereas NIb gene is very conservative.

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Genetic variability of Plum pox virus isolates in the Czech Republic

Cited by 14 publications

References 16 publications

The global phylogeny of Plum pox virus is emerging

The global phylogeny of Plum pox virus is emerging

Distribution of Plum pox virus strains in natural sources in the Czech Republic

Nucleotide Sequence of Capsid Protein Gene of Bean yellow mosaic potyvirus in Bean Plants from Al-Makhwah Governorate, Saudi Arabia

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