Genetic variability of Indonesian eggplant (Solanum melongena) based on ISSR markers

Husnudin, Uni Baroroh; Daryono, B.S.; Purnomo, Purnomo

doi:10.13057/biodiv/d201038

Cited by 11 publications

(10 citation statements)

References 31 publications

(43 reference statements)

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“…This increased variation within different chilli accessions may be due to distinct ecological conditions, adaptations, and variations in morphological characteristics in chillies. Also, polymorphism of different microsatellite repeats offers a great efficacy to identify inter-and intra-specific genetic polymorphism [99].…”

Section: Discussionmentioning

confidence: 99%

Exploring the genetic makeup and population structure among Capsicum accessions for crop improvement and breeding curriculum insights

Haq

Dubey²,

Prerna

et al. 2022

Journal of Genetic Engineering and Biotechnology

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Background Capsicum or chilli is an important crop in India which exhibits immense structural and genetic variations reflecting their intra- and inter-specific relationships. The aim of this study was to establish relationships amongst 54 Capsicum accessions through analysis of genetic and population structure using ISSR markers. Results Out of 19, successful DNA amplifications were shown by 7 ISSR primers and a total of 80 bands were identified ranging between 8 and 14 with an average of 11.43 bands/primer. A significant degree of polymorphic information content (PIC), discriminating power (DP), resolving power (RP), effective multiplex ratio (EMR), and marker index (MI) were identified as 0.39, 0.70, 6.40, 5.88, and 2.30, respectively, using ISSR markers in chillies. The cross-transferability ranged from 8.0 to 72.15% with an average of 52.63% among chillies. Amongst genetic information, grand mean values were 0.264, 0.180, 0.376, 0.296, and 0.180, which correspond to Shannon’s information index (I), expected heterozygosity (He), Nei’s gene diversity, total diversity among species (Ht), diversity within species (Hs), respectively. Further, the coefficients of gene differentiation (Gst) and gene flow (Nm) were 0.393 and 0.773, representing higher genetic variation among the population which was confirmed by analysis of molecular variance (AMOVA). Conclusion ISSR markers represented a potent system for the estimation of relationships or variation studies and generated information useful for planning crop management and improvement strategies in chilli breeding.

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Section: Discussionmentioning

confidence: 99%

Exploring the genetic makeup and population structure among Capsicum accessions for crop improvement and breeding curriculum insights

Haq

Dubey²,

Prerna

et al. 2022

Journal of Genetic Engineering and Biotechnology

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“…Actually, this class of molecular markers is based on inter-tandem repeats of short DNA sequences (Shrivastava et al 2018). These regions lie within the microsatellite repeats and offer great potential to determine intra-genomic and inter genomic differences (Shrivastava et al 2018;Husnudin et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of exotic and local eggplants (Solanum spp.) cultivated in Côte d’Ivoire based on ISSR markers

et al. 2020

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Abstract. Konan NO, Akaffou MA, Kouadio L, Akaffou DS, Mergeai G. 2020. Genetic diversity of exotic and local eggplants (Solanum spp.) cultivated in Côte d’Ivoire based on ISSR markers. Biodiversitas 21: 3651-3658. Eggplants are important vegetables in Côte d'Ivoire. Despite an increase in the cultivation of exotic genotypes and their progressive dominance over local cultivars, which could represent a threat of genetic erosion, there are not enough molecular data available on the genetic diversity of the eggplants currently cultivated in this country. This study is a contribution to the knowledge of the genetic diversity of the current eggplant cultivars grown in Côte d’Ivoire. It aimed to assess the genetic variation of nine exotic and local eggplants commonly cultivated in Côte d'Ivoire, using five inter-simple sequence repeat (ISSR) primers. The results revealed for the exotic cultivars 42 ISSR loci of which 31 were polymorphic (73.81 %), and for local cultivars 51 loci of which 49 (96.08%) were polymorphic. The Nei’s genetic diversity and Shannon’s information index of local cultivars (He = 0.3333 and I = 0.4863) were higher than those of exotic cultivars (He = 0.2000 and I = 0.2971). The Gst value and the AMOVA analysis found more than 70% of the total genetic diversity within the groups. The Jaccard’s dissimilarity coefficients between the cultivars, ranged from 0.324 to 0.966 with an average of 0.741. The clustering of genotypes with the neighbor-joining unrooted tree and PCoA analysis differentiated three main clusters with exotic cultivars grouped in cluster I and local cultivars in cluster II and III. In view of these results, the local eggplants had higher genetic diversity and constitute an interesting germplasm which needs to be conserved for potential breeding programs.

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“…ISSR (Inter-Simple Sequence Repeat) markers are highly polymorphic and useful in genetic diversity studies. ISSR can detect variations between populations that are separated by location and variations in individuals within the population [10] [11].…”

Section: Introductionmentioning

confidence: 99%

Genetic Stability Analysis Based on Inter-Simple Sequence Repeat And β-Carotene Content Analysis In Melon (Cucumis melo L. ‘GAMA Melon Parfum’)

Wibowo¹,

Rasyid²,

Maharani³

et al. 2022

International Journal on Advanced Science, Engineering and Information Technology

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The 'Gama Melon Parfum' (GMP) melons is a variety of plant breeding resulting from the Faculty of Biology's genetics and breeding laboratory, Universitas Gadjah Mada. GMP melons have a unique phenotypic character of a bitter taste and a strong aroma that has the potential to be used for cosmetics and medicine. Stability and genetic variation test are necessary to ensure the quality control of 'GMP' melons for industrial raw materials. The content of carotenoids is also important to reveal in utilizing metabolites compounds. Phenotypic character analysis was performed by comparing fruit grown conventionally and hydroponically grown on 'GMP' melons. The molecular observation method is genetic variation using the PCR-ISSR method with 5 primer ISSR and comparison with other varieties, namely 'Hikapel', 'Sky Rocket', and 'PI371795'. The data analysis used the UPGMA method, and genetic similarity was estimated using Jaccard Coefficient with MVSP 3.1 program. The method for observing β-carotene content is the UV-Vis spectrophotometric method. The results obtained showed that hydroponically grown 'GMP' melons had a relatively smaller size and faded fruit color. However, phenetically, 'GMP' melons grown both hydroponically and conventionally are in one cluster with a similarity level of 80.9%. Genetic analysis on 'GMP' melons and comparison melons showed a high level of polymorphism of 58.97%. While the results of β-carotene analysis on 'GMP' melons were 140,829 g/100 gr. It can be concluded that GMP melon has a stable genetic character. Cultivation methods and environmental factors cause changes that occur in GMP melons.

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Genetic variability of Indonesian eggplant (Solanum melongena) based on ISSR markers

Cited by 11 publications

References 31 publications

Exploring the genetic makeup and population structure among Capsicum accessions for crop improvement and breeding curriculum insights

Exploring the genetic makeup and population structure among Capsicum accessions for crop improvement and breeding curriculum insights

Genetic diversity of exotic and local eggplants (Solanum spp.) cultivated in Côte d’Ivoire based on ISSR markers

Genetic Stability Analysis Based on Inter-Simple Sequence Repeat And β-Carotene Content Analysis In Melon (Cucumis melo L. ‘GAMA Melon Parfum’)

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