Genetic variability of Fusarium solani and Fusarium oxysporum f. sp. passiflorae isolates from Pantanal, Amazon and Cerrado biomes of Mato Grosso, Brazil

Abstract

Soil fungi, Fusarium solani and Fusarium oxysporum f. sp. passiflorae are among the pathogens of greatest economic importance to the passion fruit culture. Little is known about the genetic variability of these pathogens from different regions. The genetic variability of F. solani and F. oxysporum f. sp. passiflorae isolates from Pantanal, Amazon and Cerrado of Mato Grosso State, Brazil, was studied with the help of molecular markers inter-simple sequence repeat amplification (ISSR) and interretrotransposon amplified polymorphism (IRAP). The molecular identification of the isolates was performed by partial sequencing of the internal transcribed spacer (ITS) of the rDNA region. High genetic variability among isolates of F. solani and F. oxysporum f. sp. passiflorae was found. The dendrogram generated by UPGMA divided the fungi isolates in two large groups, however, with grouping tendency according to the biome of collection only for F. oxysporum f. sp. passiflorae. There was no significant correlation between geographic distance and genetic similarity for F. oxysporum f. sp. passiflorae and F. solani.

Control of Passion Fruit Fungal Diseases Using Essential Oils Extracted from Rosemary(Rosmarinus officinalis)and Eucalyptus(Eucalyptus agglomerata)in Egerton University Main Campus Njoro, Kenya

“…The severity of the two diseases is influenced by climatic conditions of a given place which could be a contributing factor. In contrast to the findings of this study, Arielen et al [ 15 ] obtained lower values on isolation of Fusarium spp. This difference can be attributed to differences in passion fruit management practices in the two study areas.…”

“…The fungal pathogens were tested for the amylase activity using plate assay [ 15 ]. pH of the media was increased to 6.0 using 1 M HCl prior to autoclaving at 121°C for 15 min.…”

Molecular and Aggressiveness Characterization of Isolates of Fusarium solani and Fusarium oxysporum f.sp. passiflorae Associated to Passion Fruit Wilting

“…passiflorae belonging to the collection of the State University of Mato Grosso (UNEMAT) were selected for characterization of aggressiveness in P. edulis (Tables 1 and 2). All isolates were collected from plants presenting symptoms of wilting in passion fruit producing areas, including the three biomes of the state of Mato Grosso: Pantanal, Cerrado and Amazon (Carvalho et al, 2015). All isolates are preserved in the UNEMAT fungi collection in filter paper strips stored in a refrigerator at ± 5 °C.…”

“…However, there are still no passion fruit cultivars resistant to collar rot and fusariosis recorded in the Ministry of Livestock and Supply. In this context, knowledge on the pathogenicity and variability of the pathogen population is indispensable for breeding programs aiming the selection of a resistant passion flower cultivar or a hybrid in view of the complex pathosystem, where the pathogen involved presents a genetic variability, as reported by previous studies (Carvalho et al, 2015;Dariva et al, 2015;Silva et al, 2017).…”

Fusarium oxysporum f. sp. passiflorae isolates display variable virulence in Passiflora edulis Sims seedlings

“…Part of the inconsistency in the inoculation process is associated with the pathogenicity of FOP isolates. Their genetic variability is wide (Silva et al 2013b;Carvalho et al 2015) and several factors onset pathogen aggressiveness. Among these factors, the production of extracellular enzymes that interact with the host's roots (Masachis et al 2016;Galeana-Sánchez et al 2017;Mezzomo et al 2019;Ding et al 2020;Singh et al 2020), isolate geographic location, genetic variation, and environmental factors such as water stress, are highlighted (Silva et al 2013b;Lima et al 2019).…”