Genetic variability of Coffea arabica L. accessions from Ethiopia evaluated with RAPDs

Genetic diversity and structure of Ethiopian, Yemen and Brazilian Coffea arabica L. accessions using microsatellites markers

“…Previous studies have already evaluated the genetic diversity of some Ethiopian and FAO accessions through morphologic and agronomic characteristics (Montagnon and Bouharmont, 1996), RAPD markers (Lashermes et al, 1996;Anthony et al, 2001;Chaparro et al, 2004;Aga et al, 2003) and ISSR markers (Aga et al, 2005). However, in these studies only few accessions from Yemen were analyzed (Montagnon and Bouharmont, 1996;Lashermes et al, 1996;Anthony et al, 2002) and were reported different results regarding grouping and genetic structure of those accessions collected in different regions from Ethiopia and Yemen.…”

Strategies to develop polymorphic markers for Coffea arabica L.

“…Several authors have tried different molecular tools to search for polymorphic markers in coffee. These molecular techniques include RAPDs (Diniz et al 2005;Chaparro et al 2004), ISSRs (Ruas et al 2003), and SSRs (Moncada and McCouch 2004), but most have revealed the low polymorphism of the species and have shown the difficulty of finding polymorphic markers suitable for the construction of the C. arabica molecular map. Here we explored three different molecular tools, Cleaved and Amplified Polymorphic Sequences (CAPS), Single Strand Conformation Polymorphism (SSCP) and Single Nucleotide Polymorphism (SNPs), to identify the best strategy to find polymorphic markers in C. arabica, a crop with a very low genetic diversity.…”

Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion

“…Sera et al (2003) using RAPD markers associated with restriction digestion showed genetic divergence between arabica accessions of the same origin. Chaparro et al (2004) used RAPD technique for the analysis of 50 wild and semiwild accessions of C. arabica and concluded that a much larger polymorphism was present in the collection examined than previously reported in others coffee collections. In this study, we assessed the genetic relationship among arabica accessions from a germplasm collection from Brazil using RAPD associated with prior digestion of DNA with restriction enzymes.…”