Genetic variability of a population of Aedes aegypti from Paraná, Brazil, using the mitochondrial ND4 gene

Twerdochlib, Adriana Lacerda; Bonna, Ana C. Dalla; Leite, Selene S.; Chitolina, Rodrigo Faitta; Westphal, Betina; Navarro-Silva, Mário Antônio

doi:10.1590/s0085-56262012005000030

Cited by 15 publications

(33 citation statements)

References 45 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…samples from Ivory Coast, Nigeria, Cameroon, and Guinea. The levels of nucleotide diversity (π) are similar to values reported in West Africa for both COI and ND4 (Bennett et al, 2016;Paupy et al, 2008), and much lower than the values reported for East Africa (Bennett et al, 2016), America (Eskildsen et al, 2018;Jaimes-Dueñez, Arboleda, Triana-Chávez, & Gómez-Palacio, 2015;Scarpassa, Cardoza, & Cardoso, 2008;Twerdochlib et al, 2012), and Asia (Bosio et al, 2005), possibly due to multiple introduction events in those continents (Bracco, Capurro, Lourenço-de-Oliveira, & Sallum, 2007;Powell & Tabachnick, 2013).…”

Section: West African Originsupporting

confidence: 69%

Phylogeography and invasion history of Aedes aegypti, the Dengue and Zika mosquito vector in Cape Verde islands (West Africa)

Salgueiro

Serrano

Gomes

et al. 2019

Evolutionary Applications

View full text Add to dashboard Cite

Aedes‐borne arboviruses have spread globally with outbreaks of vast impact on human populations and health systems. The West African archipelago of Cape Verde had its first outbreak of Dengue in 2009, at the time the largest recorded in Africa, and was one of the few African countries affected by the Zika virus epidemic. Aedes aegypti was the mosquito vector involved in both outbreaks. We performed a phylogeographic and population genetics study of A. aegypti in Cape Verde in order to infer the geographic origin and evolutionary history of this mosquito. These results are discussed with respect to the implications for vector control and prevention of future outbreaks. Mosquitoes captured before and after the Dengue outbreak on the islands of Santiago, Brava, and Fogo were analyzed with two mitochondrial genes COI and ND4, 14 microsatellite loci and five kdr mutations. Genetic variability was comparable to other African populations. Our results suggest that A. aegypti invaded Cape Verde at the beginning of the Holocene from West Africa. Given the historic importance of Cape Verde in the transatlantic trade of the 16th–17th centuries, a possible contribution to the genetic pool of the founding populations in the New World cannot be fully discarded. However, contemporary gene flow with the Americas is likely to be infrequent. No kdr mutations associated with pyrethroid resistance were detected. The implications for vector control and prevention of future outbreaks are discussed.

show abstract

Section: West African Originsupporting

confidence: 69%

Phylogeography and invasion history of Aedes aegypti, the Dengue and Zika mosquito vector in Cape Verde islands (West Africa)

Salgueiro

Serrano

Gomes

et al. 2019

Evolutionary Applications

View full text Add to dashboard Cite

show abstract

“…In our results, genetic diversity (Hd), number of haplotypes (N) and nucleotide diversity () were lower (N = 3, Hd = 0.67 and = 0.006) than in other studies on the ND4 gene of A. aegypti. The studied Colombian A. aegypti populations were genetically structured, a trend also found in other populations from South America and Central America (GORROCHOTEGUI-ESCALANTE et al 2002, COSTA-DA-SILVA et al 2005, URDANETA-MARQUEZ et al 2008, PAUPY et al 2012, TWERDOCHLIB et al 2012, CALDERA et al 2013. The genetic structure of the studied Colombian populations occurred between regions separated by less than 17 km, similar to the patterns observed in Venezuela (distance less than 15 km between them), which suggest that gene flow is restricted (HERRERA et al 2006).…”

supporting

confidence: 61%

“…The ND4 mitochondrial gene, which codifies the subunit 4 of the NADH dehydrogenase enzyme, is an effective tool to analyze genetic population structure and colonization events in A. aegypti. Such analyses were carried out in mosquito populations from Brazil (TWERDOCHLIB et al 2012), Bolivia (PAUPY et al 2012), Peru (YÁÑEZ et al 2013), Venezuela (URDANETA-MARQUEZ et al 2008) and Mexico (GORROCHOTEGUI-ESCALANTE et al 2002). So far, only RAPDs (Random Amplified Polymorphic DNA) (OCAMPO & WESSON 2004, MEJÍA et al 2011) and more recently the mtDNA (CALDERA et al 2013) have been used to analyze the genetic structure of A. aegypti populations in Colombia.…”

mentioning

confidence: 99%

Insecticide resistance and genetic variability in natural populations of Aedes (Stegomyia) aegypti (Diptera: Culicidae) from Colombia

Aguirre-Obando¹,

Bona²,

Duque³

et al. 2015

Zoologia (Curitiba)

View full text Add to dashboard Cite

“…In the specific case of Ae. aegypti, subunit 4 (ND4) of the mitochondrial NADH gene has been shown to be an excellent tool for the analysis of the genetic differentiation of the populations of this vector [15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Genetic Variability and Evidence of Two Distinct Lineages of Aedes aegypti (Diptera, Culicidae) on São Luís Island in Maranhão, Brazil

Fraga¹,

Oliveira²,

Aragão³

et al. 2013

TOTMJ

View full text Add to dashboard Cite

Aedes aegypti is the principal vector of the yellow fever virus and the four dengue serotypes and its hemorrhagic fever viruses. The genetic variability and differentiation of four Aedes aegypti populations from São Luis Island in the Brazilian state of Maranhão was analyzed based on the sequences of a fragment of the mitochondrial ND4 gene. A total of 58 sequences of 337 bps were analyzed, revealing the existence of 10 haplotypes, of which five were considered to be unique. Haplotype diversity for the total population was 0.6273 and nucleotide diversity 0.00748. The haplotype tree produced from the data indicated the presence of two mitochondrial lineages of the dengue vector, one of which was characterized by the H6 haplotype, found only in the population from one site (Raposa), and may represent the recent introduction of this lineage to the island. The results of the AMOVA indicated that the majority of the genetic variation (74.38%) was found within populations. However, the significant Fst value of 0.2572 indicates a certain inter-population differentiation which may result in differences in the vectorial capacity of the insect, its susceptibility to the virus or even resistance to insecticides or other ecological adaptations, all of which may limit the effectiveness of programs for the control of Ae. aegypti.

show abstract

Genetic variability of a population of Aedes aegypti from Paraná, Brazil, using the mitochondrial ND4 gene

Cited by 15 publications

References 45 publications

Phylogeography and invasion history of Aedes aegypti, the Dengue and Zika mosquito vector in Cape Verde islands (West Africa)

Phylogeography and invasion history of Aedes aegypti, the Dengue and Zika mosquito vector in Cape Verde islands (West Africa)

Insecticide resistance and genetic variability in natural populations of Aedes (Stegomyia) aegypti (Diptera: Culicidae) from Colombia

Genetic Variability and Evidence of Two Distinct Lineages of Aedes aegypti (Diptera, Culicidae) on São Luís Island in Maranhão, Brazil

Contact Info

Product

Resources

About