2022
DOI: 10.3390/genes13020304
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Genetic Variability and Population Structure of the Tunisian Sicilo-Sarde Dairy Sheep Breed Inferred from Microsatellites Analysis

Abstract: This study analyzed the genetic variability, inbreeding and population structure of the Tunisian–North African dairy sheep breed, the Sicilo-Sarde (SS), created by crossing the Sarda and Comisana dairy breeds. The level of variability in the SS, considered as an endangered breed after a dramatic decrease, was assessed using 17 microsatellite markers by analyzing the two breed populations sampled from their respective cradles: SS of Beja (SSB, n = 27) and SS of Mateur (SSM, n = 25). High levels of genetic diver… Show more

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Cited by 6 publications
(9 citation statements)
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References 40 publications
(74 reference statements)
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“…Consequently, because of this moderate variation among breeds, a large part of the total genetic variability (82%) can be explained by the between-individual variability. The high number of alleles for each locus (16 to 38), as well as the PIC values (higher than 0.5), confirmed that all microsatellite markers used in this study were appropriate to evaluate the biodiversity of the Tunisian-Italian local sheep breeds, as well as proved by previous studies on native sheep breeds [ 17 , 21 , 23 , 25 ]. Microsatellites are the most frequently used markers for genotyping local farm animal breeds between 2005 and 2020 [ 39 ].…”
Section: Resultssupporting
confidence: 82%
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“…Consequently, because of this moderate variation among breeds, a large part of the total genetic variability (82%) can be explained by the between-individual variability. The high number of alleles for each locus (16 to 38), as well as the PIC values (higher than 0.5), confirmed that all microsatellite markers used in this study were appropriate to evaluate the biodiversity of the Tunisian-Italian local sheep breeds, as well as proved by previous studies on native sheep breeds [ 17 , 21 , 23 , 25 ]. Microsatellites are the most frequently used markers for genotyping local farm animal breeds between 2005 and 2020 [ 39 ].…”
Section: Resultssupporting
confidence: 82%
“…The SS is a unique North African dairy breed created early in the last century from a crossbreeding schema between the Italian Sarda and Comisana dairy breeds in the northern region of Tunisia [ 25 ]. Counting about 38,000 ewes, the SS represents 1% of the total Tunisian sheep resources [ 78 ].…”
Section: Resultsmentioning
confidence: 99%
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“…Genomic DNA extraction was carried out from 300 µL whole blood using the Wizard Genomic DNA Extraction Kit (Promega, Madison, WI, USA) following the manufacturer’s protocol. The entire number of samples was genotyped at 17 microsatellites loci panel ( Table 2 ) established from the ISAG recommended microsatellites markers [ 38 ] and from previous sheep genetic diversity studies [ 39 , 40 , 41 ]. Genotypes for all 17 microsatellite markers were determined by means of three multiplex PCR reactions using fluorescence-labeled primers in a total volume of 12.5 µL.…”
Section: Methodsmentioning
confidence: 99%