Genetic variability and phylogeny of the high‐risk HPV‐31, ‐33, ‐35, ‐52, and ‐58 in central Brazil

Raiol, Tainá; Wyant, Patrícia Soares; Amorim, Regina Maria Santos de; Cerqueira, Daniela Marreco; Milanezi, Natália von Gal; Brígido, Marcelo de Macedo; Sichero, Laura; Martins, Cláudio

doi:10.1002/jmv.21432

Cited by 50 publications

(64 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…HPV 52 in our study had variant lineages similar to those reported by previous studies. 18,19,21,23 In a Canadian study on E6-E7-LCR segment of 111 HPV 52 samples, the prototype-like group was the more common type. 18 However, the prototype-like group was observed in only two samples in our study.…”

Section: Discussionmentioning

confidence: 99%

“…19 Phylogenetic analysis of HPV 52 was constructed using LCR sequences of four samples detected in central Brazil, along with those from other parts of the world submitted to the GenBank database. 21 Four clusters were identified and two of which could be classified as Asian and European branches. The HPV 52 B variant in our study could be regarded as an Asian cluster and the A variant (prototypelike) as a European cluster.…”

Section: Epidemiologymentioning

confidence: 99%

“…16,17 The intratypic variants of HPV 16 and 18 have been studied extensively, and data on the E6, E7 and LCR genetic variations of HPV 52 or 58 have also been reported previously. [18][19][20][21][22][23] Variant lineages of carcinogenic HPV types were determined (RD Burk; personal communication) in a population-based prospective study and their associations with viral persistence and/or risk of CIN3/ CIS and cancer (CIN3þ) were evaluated using a nested casecontrol study design. 13 However, data concerning the associations between variants of HPV 52 or 58 and persistent infection of HPV or cervical neoplasia were still limited.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Unique variants of human papillomavirus genotypes 52 and 58 and risk of cervical neoplasia

Chang

Chen

Lee³

et al. 2010

Intl Journal of Cancer

View full text Add to dashboard Cite

Human papillomavirus (HPV) 52 and 58 are oncogenic HPV types prevalent in Asia. Our study aims to explore intratypic variants of HPV 52 and 58 in Taiwan. A total of 11,923 women were enrolled from seven townships in [1991][1992]. HPV DNA in their cervical cells was detected and typed by EasyChip V R HPV blot. Among 424 participants infected with HPV 52 and/or 58, nucleotide variations were determined in cervical cell samples of 406 participants by the polymerase chain reaction sequencing of the long control region, E6 and E7 genes. Nonprototype-like variants including lineages B and C were detected in 278 (99.3%) of 280 HPV 52 samples. The prototype and prototype-like group (lineage A) of HPV58 was found in 132 (98.5%) of 134 HPV 58 samples, with sublineage A1, A2 and A3 variant in 14.2, 27.6 and 56.7%, respectively. Among women infected with single HPV 52 type, the C variant (vs. B variant) was associated with an increased prevalence of cytologically diagnosed high-grade squamous intraepithelial lesion or worse lesions showing an age-adjusted odds ratio (95% confidence interval, CI) of 5.2 (1.0-27.6) and an increased prevalence of histologically confirmed high-grade cervical intraepithelial neoplasia or more severe lesions with an age-adjusted odds ratio (95% CI) of 7.6 (1.3-43.8). It was concluded that frequency distributions of HPV 52 and 58 variants in Taiwan were different from those in European and American populations. The association between C variant of HPV 52 and prevalence of cervical neoplasia needs further validation.Oncogenic human papillomavirus (HPV) is the major cause of cervical cancer. In addition to HPV 16 and 18, HPV 52 and 58 are also considered high-risk types for cervical intraepithelial lesions (CINs) and invasive carcinoma. 1,2 The unusually high prevalence of HPV 52 and 58 has been reported in Chinese populations living in China 3,4 and Taiwan. [5][6][7][8] In a previous report of our study, the prevalence of high-grade squamous intraepithelial lesion (HSIL) or worse lesions among participants with single-type infection of HPV 16, HPV 58 and HPV 52 at study entry was 31.9, 35.7 and 13.3%, respectively. 9 The participants infected with HPV 16, HPV 58 or HPV 52 had an increased prevalence of cervical cancer and carcinoma in situ (CIS).Intratypic HPV variants are characterized by the difference of less than 2% in nucleotide sequence from the prototypic L1 gene. 10 The genetic divergence among variants can differ by as much as 5% in the noncoding long control region (LCR). 11 But a study based on the complete genome analyses of HPV 16 showed that the E5-L2 intergenic region and the E4 and E5 genes are also hypervariable. 12 In a recent study, HPV variants can be classified based on their nucleotide sequence differences with most variants differing by <3%; type-specific variant lineages are defined based on a complete genome nucleotide sequence having >1% dissimilarity with the prototype and are named according to the phylogenetic tree topologies for each individual type. 13 Previous studie...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Epidemiologymentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Unique variants of human papillomavirus genotypes 52 and 58 and risk of cervical neoplasia

Chang

Chen

Lee³

et al. 2010

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…1 Clinical relevance of HPV31 variants has been rarely described, [13][14][15] although the intratypic variations in the L1, E6, E7 and long control region were recognized. [14][15][16][17][18][19][20][21] A recent study by Chen et al 22 has defined HPV31 variants as A, B and C lineages based on the whole genome analyses. This provides a basis to view the variant as a distinct phylogenetic entity and better understand the variant-related pathogenesis of cervical lesion.…”

mentioning

confidence: 99%

Association of human papillomavirus type 31 variants with risk of cervical intraepithelial neoplasia grades 2–3

Schiffman

Koutsky

et al. 2012

Intl Journal of Cancer

View full text Add to dashboard Cite

Although the lineages of human papillomavirus type 31 (HPV31) variants are recognized, their clinical relevance is unknown. The purpose of our study was to examine risk of cervical intraepithelial neoplasia Grades 2-3 (CIN2/3) by HPV31 variants. Study subjects were women who participated in the atypical squamous cells of undetermined significance and low-grade squamous intraepithelial lesion Triage Study and who had HPV31 infections detected at one or more visits. They were followed semi-annually over 2 years for detection of HPV DNA and cervical lesion. HPV31 isolates were characterized by DNA sequencing and assigned into 1 of 3 variant lineages. CIN2/3 was histologically confirmed in 127 (27.0%) of the 470 HPV31-positive women, 83 diagnosed at the first HPV31-positive visit and 44 thereafter. The odds ratio for the association of 2-year cumulative risk of CIN2/3 was 1.7 (95% CI: 1.0-2.9) for infections with A variants and 2.2 (95% CI: 1.2-3.9) for infections with B variants as compared to those with C variants. Among women without CIN2/3 at the first HPV31-positive visit, the risk of subsequent CIN2/3 was 2.2-fold greater for those with A variants (95% CI: 1.0-4.8) and 2.0-fold greater for those with B variants (95% CI: 0.9-4.9) as compared to those with C variants. Similar associations were observed when CIN3 was used as the endpoint. The findings from our study help to tag HPV31 variants that differ in risk of CIN2/3 and to explain in part why some HPV31 infections regress spontaneously and others lead to disease progression.Cervical infection with human papillomavirus (HPV) types is a necessary cause of cervical cancer and its precursor, cervical intraepithelial neoplasia Grades 2-3 (CIN2/3). 1-3 To date, >150 papillomavirus types have been fully characterized and classified, from the higher to lower taxon, as genera, species and type. 4,5 HPV types are known to differ in their tropisms and oncogenic potentials. Studies of the intratypic variation, i.e., the variant, a level below the taxon of type, have focused mainly on HPV16 and HPV18, showing that some variants were more aggressive than others. [6][7][8][9][10][11][12] HPV31 is one of the oncogenic types, most closely related to HPV16. Prevalence of some HPV types may vary geographically. Results from a recent meta-analysis (including 215 studies worldwide) have shown that on average prevalence of HPV31 infection ranks fourth, after types 16, 18 and 45, in cases of cervical cancer and second, only after type 16, in women with high-grade squamous intraepithelial lesion (referring both to cytologically detected high-grade lesions and histologically diagnosed CIN2/3 or carcinoma in situ in this analysis). 1 Clinical relevance of HPV31 variants has been rarely described, 13-15 although the intratypic variations in the L1, E6, E7 and long control region were recognized. 14-21 A recent study by Chen et al. 22 has defined HPV31 variants as A, B and C lineages based on the whole genome analyses. This provides a basis to view the variant as a distinct phylogenet...

show abstract

“…HPV-52 is phylogenetically related to HPV-16 (7), whose variants could be divided into Asian-American, African and European lineages, whose carcinogenic risks have been extensively studied (8,9). Similarly, the variants of HPV-52 could be classified as Asian and European lineages (10), with a mean sequence difference of 1.8-2.0% (11). However, limited data of HPV-52 variants is available from the mainland of China (12).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the genetic variability of human papillomavirus type 52

Chen

Luo

Lin

et al. 2012

International Journal of Molecular Medicine

View full text Add to dashboard Cite

Abstract. Human papillomavirus (HPV) type 52 is one of the high-risk HPV types. Its variants can be classified as of Asian and European lineages, while little data of HPV 52 variants are available from China. In this study, the complete E6 and L1 genes were amplified and sequenced in 79 samples from eastern Guangdong, China. In total, 21 variants were identified, 18 of which could be divided into Asian lineage and the other three were phylogenetically related to European lineage. No significant difference was found between the pathogenicity of these two lineage variants. Two HPV-52 lineages variants could be classified by high resolution melting (HRM) analysis. Thus, we believe that the application of HRM analysis would be useful for facilitating pathogenic comparisons between different HPV variants. In addition, we found that 6 females were infected with two types of HPV-52 variants. To our knowledge, this is the first time that this rare phenomenon is reported worldwide.

show abstract

Genetic variability and phylogeny of the high‐risk HPV‐31, ‐33, ‐35, ‐52, and ‐58 in central Brazil

Cited by 50 publications

References 34 publications

Unique variants of human papillomavirus genotypes 52 and 58 and risk of cervical neoplasia

Unique variants of human papillomavirus genotypes 52 and 58 and risk of cervical neoplasia

Association of human papillomavirus type 31 variants with risk of cervical intraepithelial neoplasia grades 2–3

Evaluation of the genetic variability of human papillomavirus type 52

Contact Info

Product

Resources

About