Evaluation of the genetic variability of human papillomavirus type 52

Abstract

Abstract. Human papillomavirus (HPV) type 52 is one of the high-risk HPV types. Its variants can be classified as of Asian and European lineages, while little data of HPV 52 variants are available from China. In this study, the complete E6 and L1 genes were amplified and sequenced in 79 samples from eastern Guangdong, China. In total, 21 variants were identified, 18 of which could be divided into Asian lineage and the other three were phylogenetically related to European lineage. No significant difference was found between the pathogenicity of these two lineage variants. Two HPV-52 lineages variants could be classified by high resolution melting (HRM) analysis. Thus, we believe that the application of HRM analysis would be useful for facilitating pathogenic comparisons between different HPV variants. In addition, we found that 6 females were infected with two types of HPV-52 variants. To our knowledge, this is the first time that this rare phenomenon is reported worldwide.

Whole-genome analysis of human papillomavirus genotypes 52 and 58 isolated from Japanese women with cervical intraepithelial neoplasia and invasive cervical cancer

“…3 . Although previous variant classification for HPV52/58 mostly depended on partial sequences in E6, E7, E2, L1 or LCR [ 17 , 20 – 22 ], the diagnostic SNPs described in this study will be useful for designing new PCR targets and primers to correctly assign variant lineages/sublineages of HPV52/58 in future epidemiological studies.…”

“…While HPV52/58 infections are common in East Asia, comprehensive surveys have not been conducted regarding their variant distributions in Japan. Determining the complete viral genome sequence is the most reliable and accurate procedure to assign HPV variant lineages/sublineages compared with utilizing only limited sequences in the HPV genome, which was generally done in previous studies on HPV52/58 variant classification [ 17 , 20 – 22 ]. This study thus aimed to collect the whole-genome sequences of HPV52/58 from Japanese women with CIN/ICC through next generation sequencing techniques and perform in-depth analyses of genetic variations of these particular HPVs.…”