Lineage distribution and E2 sequence variation of high-risk human papillomavirus types isolated from patients with cervical cancer in Sichuan province, China

Wu, Haijing; Enqi, Wu; Ma, Lin; Zhang, Guonan; Shi, Yu; Huang, Jianming; Xiao, Zhengguo

doi:10.1007/s00705-015-2559-z

Cited by 2 publications

(1 citation statement)

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“…In this study, lineage A was most prevalent in Jingzhou area, especially for sub-lineage A1, which was also prevalent in Yunnan, Sichuan, Zhejiang etc. [ 16 , 25 , 32 ]. The higher oncogenicity and infection rate of sub-lineage A1 may explain the reason why HPV-58 has a high incidence of cervical cancer in China.…”

Section: Discussionmentioning

confidence: 99%

Genetic variability of E6 and E7 genes of human papillomavirus type 58 in Jingzhou, Hubei Province of central China

Yang

Zhang

Luo

et al. 2022

Virol J

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Background Cervical cancer is a common malignant tumor in women, with a high mortality rate, has great harm to women’s health. Long-term and persistent infection of high-risk human papillomavirus (HR-HPV) is the main reason of the occurrence and development of cervical cancer. Methods The infection rate of HPV-58 is higher in the Jingzhou area. In this study, 172 complete HPV-58 E6-E7 sequences were amplified by polymerase chain reaction (PCR), the amplified products were sequenced, and the gene variations of HPV-58 E6-E7 were analyzed. A Neighbor-Joining phylogenetic tree was constructed by MEGA 11. The secondary structure of E6 and E7 protein was investigated. PAML X was used to analyze the selective pressure. The B cell epitopes of E6 and E7 proteins in HPV-58 were predicted by ABCpred server. Results In E6 sequences, 10 single nucleotide variants were observed, including 7 synonymous and 3 non-synonymous variants. In E7 sequences, 12 single nucleotide variants were found, including 3 synonymous variants and 9 non-synonymous variants. There are 5 novel variants. The phylogenetic analysis showed that all the E6-E7 sequences were distributed in A lineage. No positively selected site was found in E6 sequence, but G63 in E7 sequences was identified as positively selected site. Some amino acid substitutions affected multiple B cell epitopes. Conclusion Various E6 and E7 mutational data may prove useful for development of better diagnostic and vaccines for the region of Jingzhou, Hubei province of central China.

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Section: Discussionmentioning

confidence: 99%