Genetic transformation of pigeonpea with rice chitinase gene

Kumar, Suneel; Kumar, B. Kiran; Sharma, Kiran K.; Devi, Prathibha

doi:10.1111/j.1439-0523.2004.01028.x

Cited by 40 publications

(23 citation statements)

References 17 publications

(15 reference statements)

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“…hygromycin was used as a selection pressure for the co-cultivated explants which strengthened the previous findings (Kumar et al, 2004;Modgil & Sharma 2009, Sharma et al, 2012.…”

Section: Discussionsupporting

confidence: 62%

IN VITRO REGENERATION AND TRANSFORMATION OF APPLE (Malus domestica Borkh.) ROOTSTOCK MALLING 7 USING RICE CHITINASE GENE

Sharma¹,

Modgil²,

Sharma³

2017

JEBAS

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In most of the fruit crops, the low frequency of Agrobacterium mediated genetic transformation hampers the respective transgenics production. Hence, it is pre-requisite to understand and optimize the different parameters involved directly or indirectly in Agrobacterium mediated transformation. A. tumefaciens strain LBA4404 harboring the transforming vector pCAMBARchi11 containing the rice chitinase gene (chi11) and hygromycin phosphotransferase (hpt) and phosphinothricin acetyl transferase (bar) genes was used for carrying out genetic transformation of apple (Malus x domestica Borkh.) rootstock M7. Two explants viz. leaf and internodal segments were involved in regeneration and resulted 24.37% and 60.58% shoot regeneration, respectively. The obtained putative transformants were selected on full strength MS medium containing 5 mg l -1 hygromycin and 500 mg l -1 cefotaxime. Two days pre-culturing and 96 hours co-cultivation was found effective for procuring maximum number of hygromycin resistant shoots. Putative transgenic shoots were multiplied separately and rooted on root induction medium containing 5 mg l -1 hygromycin. Further, PCR analysis was done using chitinase gene specific primers and two apple transgenic lines confirmed the integration of chi11 gene by yielding 237 bp and 584 bp amplified products, respectively.

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“…hygromycin was used as a selection pressure for the co-cultivated explants which strengthened the previous findings (Kumar et al, 2004;Modgil & Sharma 2009, Sharma et al, 2012.…”

Section: Discussionsupporting

confidence: 62%

IN VITRO REGENERATION AND TRANSFORMATION OF APPLE (Malus domestica Borkh.) ROOTSTOCK MALLING 7 USING RICE CHITINASE GENE

Sharma¹,

Modgil²,

Sharma³

2017

JEBAS

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“…The chitinase gene was successfully transformed and expressed in tobacco (Brogue et al 1991), rice (Lin et al 1995), strawberry (Asao et al 1997), pigeon pea (Kumar et al 2004), cotton (Tohidfar et al 2005(Tohidfar et al , 2009, taro (He et al 2008), and grapevine (Nirala et al 2010). Several reports have indicated the potential of chitinase gene in enhancing the resistance against sheath blight and/or sheath rot pathogens in rice (Lin et al 1995, Nishizawa et al 1999, Datta et al 2000, 2001, Itoh et al 2003, Kim et al 2003, Sridevi et al 2003, Kalpana et al 2006, Maruthasalam et al 2007, Nandakumar et al 2007.…”

Section: Discussionmentioning

confidence: 98%

Stability of sheath blight resistance in transgenic ASD16 rice lines expressing a rice chi11 gene encoding chitinase

Rajesh¹,

Maruthasalam²,

Kalpana³

et al. 2016

Biologia plant.

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Development of transgenic plants by introducing defense genes is one of the strategies to engineer disease resistance. Transgenic ASD16 rice plants harbouring rice chitinase chi11 gene, belonging to a PR-3 group of defense gene conferring sheath blight (Rhizoctonia solani Kuhn) resistance, were used in this study. Three T 2 homozygous lines (ASD16-4-1-1, 5-1-1, and 6-1-1) were identified from seven putative (T 0 ) transgenic lines expressing chi11 using Western blotting analysis. The inheritance of sheath blight resistance in those lines was studied over generations. The stability of chi11 expression up to T 4 generation in all the three homozygous lines was proved by Western blot and the stability of sheath blight resistance in the homozygous lines was proved up to T 4 generation using detached leaf and intact leaf sheath assays. Among the three homozygous lines tested, ASD16-4-1-1 showed consistent results in all the generations and gave a better protection against the sheath blight pathogen than the other two lines.

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“…Therefore, based on the above results, 10.0 mg/l hygromycin was used and demonstrated for the selection of transformed cells to be an effective selection agent in Agrobacteriummediated genetic transformation studies in lettuce. Hygromycin has been reported for the selection of transformed and non-transformed explants with very low frequency of selection escape in lettuce (Enkhchimeg et al, 2005;Dias et al, 2006;Deng et al, 2007) and also in many other crops like Banana (Maziah et al, 2007), pigeon pea plants (Kumar et al, 2004) and American ginseng plants (Chen and Punja, 2002). For successful Agrobacterium-mediated transformation, elimination of bacteria from culture is necessary after the co-cultivation period.…”

Section: Resultsmentioning

confidence: 99%

Agrobacterium-Mediated Fungal Resistance Gene Transfer Studies Pertaining to Antibiotic Sensitivity on Cultured Tissues of Lettuce (Lactuca sativa L. cv. Solan kriti)

Sharma¹,

Srivastava²

2017

Int.J.Curr.Microbiol.App.Sci

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Genetic transformation of pigeonpea with rice chitinase gene

Cited by 40 publications

References 17 publications

IN VITRO REGENERATION AND TRANSFORMATION OF APPLE (Malus domestica Borkh.) ROOTSTOCK MALLING 7 USING RICE CHITINASE GENE

IN VITRO REGENERATION AND TRANSFORMATION OF APPLE (Malus domestica Borkh.) ROOTSTOCK MALLING 7 USING RICE CHITINASE GENE

Stability of sheath blight resistance in transgenic ASD16 rice lines expressing a rice chi11 gene encoding chitinase

Agrobacterium-Mediated Fungal Resistance Gene Transfer Studies Pertaining to Antibiotic Sensitivity on Cultured Tissues of Lettuce (Lactuca sativa L. cv. Solan kriti)

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