Stability of sheath blight resistance in transgenic ASD16 rice lines expressing a rice chi11 gene encoding chitinase

Rajesh, T.; Maruthasalam, S.; Kalpana, K.; Poovannan, K.; Kumar, K. K.; Kokiladevi, E.; Sudhakar, D.; Samiyappan, R.; Balasubramanian, P.

doi:10.1007/s10535-016-0594-6

Cited by 15 publications

(5 citation statements)

References 30 publications

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“…Moreover, the number of infection cushions formed on leaves was significantly lesser in transgenic lines expressing tlp and their F2 derivatives as compared to non-transgenic control. Similar kinds of results were obtained in detached leaf assays by earlier workers (Kumar et al 2003, Kalpana et al 2006, Maruthasalam et al 2007, Rajesh et al 2016.…”

Section: Discussionsupporting

confidence: 88%

“…In earlier reports, significantly lesser HRLH% and total lesion spread in transgenic lines expressing chi11 and/or tlp were reported (Kumar et al 2003, Kalpana et al 2006, Maruthasalam et al 2007. Recently, Rajesh et al (2016) has demonstrated the inheritance of sheath blight resistance (upto T4 generation) in transgenic ASD16 rice plants expressing a chitinase gene.…”

Section: Discussionmentioning

confidence: 92%

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Pyramiding insect and disease resistance in an elite indica rice cultivar asd16

Rajesh¹,

Maruthasalam²,

Kalpana³

et al. 2020

Biol. plant.

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Pyramiding transgenes of interest is one of the strategies to engineer multiple stress resistance in crop plants. Transgenic plants which stably express different genes can be hybridized to bring these genes together in one plant. Transgenic rice (Oryza sativa L. cv. ASD 16) plants harbouring genes Xa21 (conferring bacterial blight resistance), tlp (conferring resistance to sheath blight), or gna (conferring resistance to brown planthopper) were used in hybridization experiments. Sexual hybridization was carried out in two different gene combinations: Xa21 × gna and tlp × gna. Molecular analyses were carried out to confirm the presence of transgenes. In F1 generation, lines harbouring either gene in each of the crosscombination were selected and forwarded to F2 generation. The presence of genes in F2 generation was confirmed by PCR, Southern blot hybridization, and Western blotting. The F2 progeneis harbouring Xa21 and gna exhibited resistance against bacterial blight and moderate resistance against brown planthopper. Similarly, the F2 lines of tlp and gna combination provided resistance against sheath blight and moderate resistance against brown planthopper. The level of resistance observed in pyramided lines for insect or pathogens was comparable to the resistance observed in their parental lines. Our study shows that pyramiding genes by hybridization between transgenic plants could be one of the strategies to develop cultivars with multiple biotic stress resistances.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 92%

Pyramiding insect and disease resistance in an elite indica rice cultivar asd16

Rajesh¹,

Maruthasalam²,

Kalpana³

et al. 2020

Biol. plant.

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“…The control measures include the breeding of disease-resistant varieties, as well as the application of chemical, biological, or RNA pesticides. As China has no diseaseresistant rice varieties, chemical pesticides are mainly used to control rice sheath blight (Rajesh et al, 2016). However, due to the lack of disease-resistant resources of rice germplasm, Rhizoctonia solani has high genetic variability and wide host affinity, and can survive from one crop season to the next by forming dormant sclerotia, which increases the difficulty of controlling rice ShB.…”

Section: Introductionmentioning

confidence: 99%

Progress in rice sheath blight resistance research

Chen

Xuan

et al. 2023

Front. Plant Sci.

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Rice sheath blight (ShB) disease poses a major threat to rice yield throughout the world. However, the defense mechanisms against ShB in rice remain largely unknown. ShB resistance is a typical quantitative trait controlled by multiple genes. With the rapid development of molecular methods, many quantitative trait loci (QTLs) related to agronomic traits, biotic and abiotic stresses, and yield have been identified by genome-wide association studies. The interactions between plants and pathogens are controlled by various plant hormone signaling pathways, and the pathways synergistically or antagonistically interact with each other, regulating plant growth and development as well as the defense response. This review summarizes the regulatory effects of hormones including auxin, ethylene, salicylic acid, jasmonic acid, brassinosteroids, gibberellin, abscisic acid, strigolactone, and cytokinin on ShB and the crosstalk between the various hormones. Furthermore, the effects of sugar and nitrogen on rice ShB resistance, as well as information on genes related to ShB resistance in rice and their effects on ShB are also discussed. In summary, this review is a comprehensive description of the QTLs, hormones, nutrition, and other defense-related genes related to ShB in rice. The prospects of targeting the resistance mechanism as a strategy for controlling ShB in rice are also discussed.

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“…alit has also been reported that there was a negative correlation between the mycelial growth of R. solani and chitinase activity in the antifungal assay conducted with recombinant LOC_Os11g47510, chitinase protein ( Richa et al, 2016 ). Very recently, Rajesh et al (2016) analyzed the sheath blight resistance in transgenic ASD16 rice lines expressing a rice chi11 gene encoding chitinase and found that transgenic line ASD16-4-1-1 gave a better protection against the sheath blight pathogen than the other two lines.…”

Section: Discussionmentioning

confidence: 99%

Novel Chitinase Gene LOC_Os11g47510 from Indica Rice Tetep Provides Enhanced Resistance against Sheath Blight Pathogen Rhizoctonia solani in Rice

et al. 2017

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Sheath blight disease (ShB), caused by the fungus Rhizoctonia solani Kühn, is one of the most destructive diseases of rice (Oryza sativa L.), causing substantial yield loss in rice. In the present study, a novel rice chitinase gene, LOC_Os11g47510 was cloned from QTL region of R. solani tolerant rice line Tetep and used for functional validation by genetic transformation of ShB susceptible japonica rice line Taipei 309 (TP309). The transformants were characterized using molecular and functional approaches. Molecular analysis by PCR using a set of primers specific to CaMv 35S promoter, chitinase and HptII genes confirmed the presence of transgene in transgenic plants which was further validated by Southern hybridization. Further, qRT-PCR analysis of transgenic plants showed good correlation between transgene expression and the level of sheath blight resistance among transformants. Functional complementation assays confirmed the effectiveness of the chitinase mediated resistance in all the transgenic TP309 plants with varying levels of enhanced resistance against R. solani. Therefore, the novel chitinase gene cloned and characterized in the present study from the QTL region of rice will be of significant use in molecular plant breeding program for developing sheath blight resistance in rice.

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Stability of sheath blight resistance in transgenic ASD16 rice lines expressing a rice chi11 gene encoding chitinase

Cited by 15 publications

References 30 publications

Pyramiding insect and disease resistance in an elite indica rice cultivar asd16

Pyramiding insect and disease resistance in an elite indica rice cultivar asd16

Progress in rice sheath blight resistance research

Novel Chitinase Gene LOC_Os11g47510 from Indica Rice Tetep Provides Enhanced Resistance against Sheath Blight Pathogen Rhizoctonia solani in Rice

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