Genetic Transformation of &lt;i&gt;Brassica napus&lt;/i&gt; with &lt;i&gt;MSI-99m&lt;/i&gt; Gene Increases Resistance in Transgenic Plants to &lt;i&gt;Sclerotinia sclerotiorum&lt;/i&gt;

Sang, Xuelian; Jue, Dengwei; Liu, Yang; XianJin, Bai; Chen, Min; Yang, Qing

doi:10.5376/mpb.2013.04.0030

Cited by 5 publications

(4 citation statements)

References 23 publications

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“…The defense response was initiated in transgenics with an increase in H 2 O 2 and PR-1 gene but PDF 1.2 remained comparatively inactive. Tolerance to SSR was seen in B. napus transformed with the MSI-99m gene which belongs to the magainins class of antimicrobial peptides and is responsible for broad-spectrum resistance against bacteria and fungi ( Sang et al., 2013 ). Kheiri et al.…”

Section: Sclerotinia Stem Rotmentioning

confidence: 99%

Fungal effectors versus defense-related genes of B. juncea and the status of resistant transgenics against fungal pathogens

2023

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Oilseed brassica has become instrumental in securing global food and nutritional security. B. juncea, colloquially known as Indian mustard, is cultivated across tropics and subtropics including Indian subcontinent. The production of Indian mustard is severely hampered by fungal pathogens which necessitates human interventions. Chemicals are often resorted to as they are quick and effective, but due to their economic and ecological unsustainability, there is a need to explore their alternatives. The B. juncea-fungal pathosystem is quite diverse as it covers broad-host range necrotrophs (Sclerotinia sclerotiorum), narrow-host range necrotrophs (Alternaria brassicae and A. brassicicola) and biotrophic oomycetes (Albugo candida and Hyaloperonospora brassica). Plants ward off fungal pathogens through two-step resistance mechanism; PTI which involves recognition of elicitors and ETI where the resistance gene (R gene) interacts with the fungal effectors. The hormonal signalling is also found to play a vital role in defense as the JA/ET pathway is initiated at the time of necrotroph infection and SA pathway is induced when the biotrophs attack plants. The review discuss the prevalence of fungal pathogens of Indian mustard and the studies conducted on effectoromics. It covers both pathogenicity conferring genes and host-specific toxins (HSTs) that can be used for a variety of purposes such as identifying cognate R genes, understanding pathogenicity and virulence mechanisms, and establishing the phylogeny of fungal pathogens. It further encompasses the studies on identifying resistant sources and characterisation of R genes/quantitative trait loci and defense-related genes identified in Brassicaceae and unrelated species which, upon introgression or overexpression, confer resistance. Finally, the studies conducted on developing resistant transgenics in Brassicaceae have been covered in which chitinase and glucanase genes are mostly used. The knowledge gained from this review can further be used for imparting resistance against major fungal pathogens.

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Section: Sclerotinia Stem Rotmentioning

confidence: 99%

Fungal effectors versus defense-related genes of B. juncea and the status of resistant transgenics against fungal pathogens

2023

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“…In Brassica crops, there are several transformations with genes coding for this type of protein, which have resulted in important inhibitions of pathogen growth and the development of the disease, thanks to a powerful membrane antagonism. Regarding α-helical peptides, we found effective rapeseed transgenic lines against L. maculans [66], the transformation of B. juncea with the gene coding for a cecropin-melittin cationic peptide has been effective against A. brassicae and S. sclerotiorum [53], and also B. napus with a magainin II analogue against S. sclerotiorum [79]. The simultaneous transformation of cauliflower with a cecropin from Antheraea polyphemus and a magainin from Xenopus laevis against Xcc [46] is effective as well.…”

Section: Recent Progress Of Transgenic Research In Brassica Species Against Pathogensmentioning

confidence: 99%

Development of Transgenic Brassica Crops against Biotic Stresses Caused by Pathogens and Arthropod Pests

Poveda

Francisco

Cartea

et al. 2020

Plants

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The Brassica genus includes one of the 10 most agronomically and economically important plant groups in the world. Within this group, we can find examples such as broccoli, cabbage, cauliflower, kale, Brussels sprouts, turnip or rapeseed. Their cultivation and postharvest are continually threatened by significant stresses of biotic origin, such as pathogens and pests. In recent years, numerous research groups around the world have developed transgenic lines within the Brassica genus that are capable of defending themselves effectively against these enemies. The present work compiles all the existing studies to date on this matter, focusing in a special way on those of greater relevance in recent years, the choice of the gene of interest and the mechanisms involved in improving plant defenses. Some of the main transgenic lines developed include coding genes for chitinases, glucanases or cry proteins, which show effective results against pathogens such as Alternaria brassicae, Leptosphaeria maculans or Sclerotinia sclerotiorum, or pests such as Lipaphis erysimi or Plutella xylostella.

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“…Additionally, two NAC genes ( BnNAC2 and BnNAC5 ) induced by high-salinity, drought, and abscisic acid were identified in 7-day-old seedlings of ZY821, and BnNAC2 was revealed to preferentially expressed in flowers, whereas BnNAC5 accumulated at the highest level in stems ( Zhong et al, 2012 ). The MSI-99m gene, a synthesized magainin II analog with inhibitory effects to microbial organisms, was transferred into ZY821 to increase resistance to S. sclerotiorum ( Sang et al, 2013 ). However, there was currently not enough stem transcriptome information of ZY821 and comparative transcriptome analysis of different tissues, which limited further functional genomics research on this B. napus cultivar.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Stem and Globally Comparison with Other Tissues in Brassica napus

et al. 2016

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Brassica napus is one of the most important oilseed crops in the world. However, there is currently no enough stem transcriptome information and comparative transcriptome analysis of different tissues, which impedes further functional genomics research on B. napus. In this study, the stem transcriptome of B. napus was characterized by RNA-seq technology. Approximately 13.4 Gb high-quality clean reads with an average length of 100 bp were generated and used for comparative transcriptome analysis with the existing transcriptome sequencing data of roots, leaves, flower buds, and immature embryos of B. napus. All the transcripts were annotated against GO and KEGG databases. The common genes in five tissues, differentially expressed genes (DEGs) of the common genes between stems and other tissues, and tissue-specific genes were detected, and the main biochemical activities and pathways implying the common genes, DEGs and tissue-specific genes were investigated. Accordingly, the common transcription factors (TFs) in the five tissues and tissue-specific TFs were identified, and a TFs-based regulation network between TFs and the target genes involved in ‘Phenylpropanoid biosynthesis’ pathway were constructed to show several important TFs and key nodes in the regulation process. Collectively, this study not only provided an available stem transcriptome resource in B. napus, but also revealed valuable comparative transcriptome information of five tissues of B. napus for future investigation on specific processes, functions and pathways.

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Genetic Transformation of <i>Brassica napus</i> with <i>MSI-99m</i> Gene Increases Resistance in Transgenic Plants to <i>Sclerotinia sclerotiorum</i>

Cited by 5 publications

References 23 publications

Fungal effectors versus defense-related genes of B. juncea and the status of resistant transgenics against fungal pathogens

Fungal effectors versus defense-related genes of B. juncea and the status of resistant transgenics against fungal pathogens

Development of Transgenic Brassica Crops against Biotic Stresses Caused by Pathogens and Arthropod Pests

Transcriptome Analysis of Stem and Globally Comparison with Other Tissues in Brassica napus

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