Development of Transgenic Brassica Crops against Biotic Stresses Caused by Pathogens and Arthropod Pests

Poveda, Jorge; Francisco, Marta; Cartea, María Elena; Velasco, Pablo

doi:10.3390/plants9121664

Cited by 18 publications

(3 citation statements)

References 142 publications

(94 reference statements)

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“…Alternaria spp., Fusarium oxysporum , Albuga candia , and Leptosphaeria maculans are the most prevalent fungal diseases that affect Brassica [ 36 ]. Brassica crops are susceptible to bacterial rot disease as well [ 37 ]. Similarly, salinity and drought among abiotic stresses have the highest impact on Brassica yield [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

Analyses of Lysin-motif Receptor-like Kinase (LysM-RLK) Gene Family in Allotetraploid Brassica napus L. and Its Progenitor Species: An In Silico Study

Abedi

Hajiahmadi

Kordrostami

et al. 2021

Cells

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The LysM receptor-like kinases (LysM-RLKs) play a crucial role in plant symbiosis and response to environmental stresses. Brassica napus, B. rapa, and B. oleracea are utilized as valuable vegetables. Different biotic and abiotic stressors affect these crops, resulting in yield losses. Therefore, genome-wide analysis of the LysM-RLK gene family was conducted. From the genome of the examined species, 33 LysM-RLK have been found. The conserved domains of Brassica LysM-RLKs were divided into three groups: LYK, LYP, and LysMn. In the Brassica LysM-RLK gene family, only segmental duplication has occurred. The Ka/Ks ratio for the duplicated pair of genes was less than one indicating that the genes’ function had not changed over time. The Brassica LysM-RLKs contain 70 cis-elements, indicating that they are involved in stress response. 39 miRNA molecules were responsible for the post-transcriptional regulation of 12 Brassica LysM-RLKs. A total of 22 SSR loci were discovered in 16 Brassica LysM-RLKs. According to RNA-seq data, the highest expression in response to biotic stresses was related to BnLYP6. According to the docking simulations, several residues in the active sites of BnLYP6 are in direct contact with the docked chitin and could be useful in future studies to develop pathogen-resistant B. napus. This research reveals comprehensive information that could lead to the identification of potential genes for Brassica species genetic manipulation.

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Section: Introductionmentioning

confidence: 99%

Analyses of Lysin-motif Receptor-like Kinase (LysM-RLK) Gene Family in Allotetraploid Brassica napus L. and Its Progenitor Species: An In Silico Study

Abedi

Hajiahmadi

Kordrostami

et al. 2021

Cells

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“…Further research utilizing RT-PCR validated that these chitinase genes were induced after wounding and exogenous treatments of jasmonic acid and salicylic acid similar to Alternaria infection [103]. A recent review summarized that the chitinases, glucanases or cry proteins provide broad-spectrum resistance against some major diseases including Alternaria blight and blackleg [104].…”

Section: Genetic Transformation For Alternaria Resistancementioning

confidence: 99%

Epidemiology, Genetics and Resistance of Alternaria Blight in Oilseed Brassica

Jyoti¹,

Sultana²,

Hassan³

et al. 2021

Brassica Breeding and Biotechnology [Working Title]

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Alternaria blight is one of the most deadly diseases of oilseed Brassica. This recalcitrant disease causes up to 50% yield loss across the globe. The disease is mainly caused by Alternaria brassicae and Alternaria brassicicola. These pathogens lack sexual stages and survive as conidia or condiospores on the debris of previous crops and susceptible weeds. Developing resistant oilseed Brassica cultivars to this disease has become a prime concern for researchers over the years. In absence of resistant oilseed Brassica cultivar, identification and introgression of resistance related genes can be a potential source for Alternaria blight resistance. As resistance toward Alternaria blight is governed by polygenes, intercrossing between the tolerant genotypes and subsequent selection will be the most appropriate way to transfer the quantitative resistance. For that reason, future breeding goal should focus on screening of germplasms for selecting genotypes containing resistance genes and structural features that favors resistance, like thick epicuticular wax, biochemical components such as phenols, phytoalexins and lower soluble sugars, reducing sugars and soluble nitrogen. Selected genotypes should be brought under appropriate breeding programs for attaining Alternaria blight resistance.

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“…Brassica genus, belonging to the Brassicaceae family (also known as Cruciferae), includes several crops with a high economic interest, cultivated for their edible roots, leaves, stems, buds, flowers, mustard and oilseeds [1]. The principal vegetable species belonging to the Brassica genus are Brassica napus (i.e., rapeseed and leaf rape), Brassica oleracea (i.e., broccoli, cabbage, cauliflower, kale, Brussels sprouts, etc.…”

Section: Introductionmentioning

confidence: 99%

Trichoderma hamatum Increases Productivity, Glucosinolate Content and Antioxidant Potential of Different Leafy Brassica Vegetables

Velasco

Rodríguez

Soengas

et al. 2021

Plants

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Brassica crops include important vegetables known as “superfoods” due to the content of phytochemicals of great interest to human health, such as glucosinolates (GSLs) and antioxidant compounds. On the other hand, Trichoderma is a genus of filamentous fungi that includes several species described as biostimulants and/or biological control agents in agriculture. In a previous work, an endophytic strain of Trichoderma hamatum was isolated from kale roots (Brassica oleracea var. acephala), describing its ability to induce systemic resistance in its host plant. In the present work, some of the main leafy Brassica crops (kale, cabbage, leaf rape and turnip greens) have been root-inoculated with T. hamatum, having the aim to verify the possible capacity of the fungus as a biostimulant in productivity as well as the foliar content of GSLs and its antioxidant potential, in order to improve these “superfoods”. The results reported, for the first time, an increase in the productivity of kale (55%), cabbage (36%) and turnip greens (46%) by T. hamatum root inoculation. Furthermore, fungal inoculation reported a significant increase in the content of total GSLs in cabbage and turnip greens, mainly of the GSLs sinigrin and gluconapin, respectively, along with an increase in their antioxidant capacity. Therefore, T. hamatum could be a good agricultural biostimulant in leafy Brassica crops, increasing the content of GSLs and antioxidant potential of great food and health interest.

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Development of Transgenic Brassica Crops against Biotic Stresses Caused by Pathogens and Arthropod Pests

Cited by 18 publications

References 142 publications

Analyses of Lysin-motif Receptor-like Kinase (LysM-RLK) Gene Family in Allotetraploid Brassica napus L. and Its Progenitor Species: An In Silico Study

Analyses of Lysin-motif Receptor-like Kinase (LysM-RLK) Gene Family in Allotetraploid Brassica napus L. and Its Progenitor Species: An In Silico Study

Epidemiology, Genetics and Resistance of Alternaria Blight in Oilseed Brassica

Trichoderma hamatum Increases Productivity, Glucosinolate Content and Antioxidant Potential of Different Leafy Brassica Vegetables

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