Genetic Transformation of a High Molecular Weight Glutenin (<i>Glu-1Dx5</i>) to Rice cv. Fatmawati

Wada, Yasuhiko; Carsono, Nono; Tong, Ly; Yoshida, Tomohiko

doi:10.1626/pps.12.341

Cited by 8 publications

(4 citation statements)

References 22 publications

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“…Optimization of in vitro culture system will support production of regenerable embryonic callus as a target tissue for rice genetic transformation. Callus or mass of undifferentiated cells, are excellent sources for in vitro regeneration and production of transgenic rice (Carsono and Yoshida 2008;Wada et al 2009). Several studies on the development of efficient in vitro culture system in rice i.e., from callus induction, multiplication, and plant regeneration, have been previously reported (Carsono and Yoshida 2006;Din et al 2016;Mostafiz and Wagiran 2018).…”

Section: Introductionmentioning

confidence: 99%

Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes

et al. 2021

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Abstract. Carsono N, Juwendah E, Liberty, Sari S, Damayanti F, Rachmadi M. 2021. Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes. Biodiversitas 22: 2555-2560. The development of callus in the course of transgenic rice avoids the somaclonal variants. To obtain a high number of normal phenotypes and a low number of somaclonal variants requires an appropriate 2,4-D concentration. In this study, we obtained the best callus induction time and a high number of green plant regeneration for three responsive rice genotypes on different 2,4-D concentrations in NB5 medium. The mature seeds of rice embryos were used as explants. A completely randomized factorial design was applied with four levels of 2,4-D concentrations (0, 1, 3, and 5 ppm), two levels of induction time (one and two weeks), and three rice genotypes (cv. Fatmawati, Nipponbare, and Kitaake). The study revealed that there was no interaction effect among genotype, 2,4-D concentration, and callus induction time. Three rice genotypes performed best in callus proliferation and regeneration. One-week callus induction time showed higher callus growth capacity (CGC) as compared to two-week callus induction time. Shoot regeneration capacity (SRC) was independently affected by genotype as well as by callus induction time. The interaction effect between 2,4-D concentration and callus induction time was observed on plant regeneration capacity (PRC). Without the addition of 2,4-D and 1 ppm of 2,4-D, the green plant regeneration capacity (GRC) was comparatively higher. Addition of 2,4-D showed a significant effect, especially at the plant regeneration stage. We found that one-week callus induction was the best treatment for callus proliferation and plant regeneration. We recommend the use of one-week callus induction and 1 ppm of 2,4-D for rice callus proliferation (sub-culture) and subsequent plant regeneration.

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Section: Introductionmentioning

confidence: 99%

Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes

et al. 2021

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“…Regulation of the expression of HMW‐GS primarily occurs at the transcriptional level, involving cis ‐acting motifs in the HMW‐GS promoters and trans ‐acting transcription factors (Shewry and Halford, ). The wheat HMW glutenin gene promoter has been functionally analyzed in wheat (Lamacchia et al ., ), maize (Sangtong et al ., ), rice (Wada et al ., ), Brachypodium (Thilmony et al ., ) and tobacco (Robert et al ., ), and a 40‐base‐pair region located 186 base pairs upstream of the start site acts as an enhancer necessary for endosperm‐specific transcription in transgenic tobacco plants (Thomas and Flavell, ). Tests with reporter genes in the seeds of each of these plants demonstrate that the native wheat TaGLU‐1 promoters can support expression in the endosperm.…”

Section: Introductionmentioning

confidence: 99%

The wheat transcription factor TaGAMyb recruits histone acetyltransferase and activates the expression of a high‐molecular‐weight glutenin subunit gene

et al. 2015

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SUMMARYGlutenin proteins in wheat (Triticum aestivum L.) flour confer unique viscoelastic properties to dough products and, therefore, the concentration and composition of the glutenin proteins determine its end-use value. However, the mechanisms governing the glutenin gene expression remain elusive. In this study, we report that wheat TaGAMyb activates the high-molecular-weight glutenin subunit genes (TaGLU) through recruiting the histone acetyltransferase GCN5. By sequencing the promoters of TaGLU-1 genes from 40 modern wheat cultivars, we identified eight types of TaGAMyb binding motifs and verified these by electrophoretic mobility shift assays. The number of TaGAMyb binding motifs in TaGLU-1 genes is correlated with the abundance of glutenin in different cultivars. Chromatin immunoprecipitation plus polymerase chain reaction (ChIP-PCR) analysis reveals that TaGCN5 directly targets the promoters of TaGLU-1 genes in wheat endosperm. We find that TaGAMyb physically interacts with the wheat histone acetyltransferase TaGCN5 and also interacts with Arabidopsis thaliana AtGCN5. TaGAMyb ectopically expressed in Arabidopsis binds to the TaGLU-1Dy promoter on a TaGLU-1Dy transgene and activates its expression. AtGCN5 also targets the TaGLU-1Dy transgene and is involved in the establishment of acetylation at H3K9 and H3K14. These results demonstrate that TaGAMyb plays a dual role in activating expression of glutenin gene by directly binding to the TaGLU promoter and by recruiting GCN5 to modulate histone acetylation during wheat endosperm development.

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“…The Glu-1Dx5 gene, an allele that encodes a high molecular weight glutenin subunit Dx5 and is responsible for dough elasticity and functionality of bread wheat, has been successfully transferred into the genome of rice variety Fatmawati, by using particle bombardment (Wada et al 2009). The gene was isolated from bread wheat cv.…”

Section: Introductionmentioning

confidence: 99%

Agronomic characteristics and genetic relationship of putative transgenic rice lines of cv. Fatmawati with the Glu-1Dx5 transgene

et al. 2022

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Abstract. Carsono N, Prayoga GI, Sari S, Rachmadi M. 2021. Agronomic characteristics and genetic relationship of putative transgenic rice lines of cv. Fatmawati with the Glu-1Dx5 transgene. Biodiversitas 23: 291-298. Transgenic rice lines cv. Fatmawati with the Glu-1Dx5 transgene (encoding high molecular weight glutenin sub-unit Dx5 from bread wheat) has been produced in order to improve dough functionality of rice flour. These lines have reached T1 (transformant-1) dan T2 (transformant-2) generations. Some transgenic rice plants obtained from in vitro culture and transformation events frequently show phenotypic and genotypic variations from the original plants, thus evaluation of agronomic traits of these transgenic rice is highly important in order to obtain rice genotypes with the best agronomic traits that will be integrated into rice breeding program. Nine transgenic rice lines and two check lines, i.e. seed-derived and callus-derived rice lines, were used in this experiment. A comparison between transgenic rice traits with those of non-transgenic checks was done by student’s t-test and relationship among transgenic rice lines was evaluated by UPGMA (Unweighted Pair Group Method with Mean Arithmetic) method using NT Sys (Numerical Taxonomy and Multivariate Analysis System). Results indicated that agronomic traits of transgenic rice lines were similar to those of non-transgenic check except for number of productive tillers (T2-11, T2-12, T2-20), the maximum number of tillers (T2-7, T2-11, T2-12, T2-20), number of filled grains (T1-45) and days to maturity (all T2 lines). It suggests that somaclonal variations, gene insertional effect, genetic and epigenetic mutations might occur. Genetic relationship between putative transgenic and non-transgenic checks was closely related, although somaclonal variants were found. The selected transgenic rice lines will be incorporated into rice breeding programs.

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Genetic Transformation of a High Molecular Weight Glutenin (Glu-1Dx5) to Rice cv. Fatmawati

Cited by 8 publications

References 22 publications

Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes

Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes

The wheat transcription factor TaGAMyb recruits histone acetyltransferase and activates the expression of a high‐molecular‐weight glutenin subunit gene

Agronomic characteristics and genetic relationship of putative transgenic rice lines of cv. Fatmawati with the Glu-1Dx5 transgene

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