The wheat transcription factor Ta<scp>GAM</scp>yb recruits histone acetyltransferase and activates the expression of a high‐molecular‐weight glutenin subunit gene

Guo, Weiwei; Yang, Hua; Yong-qiang, Liu; Gao, Yujiao; Ni, Zhongfu; Peng, Huiru; Xin, Mingming; Hu, Zhaorong; Sun, Qixin; Yao, Yingyin

doi:10.1111/tpj.13003

Cited by 53 publications

(57 citation statements)

References 43 publications

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“…() identified TaFUSCA3, a B3 TF, which binds an RY motif in the GluB1‐1 promoter, activating the promoter and interacting with SPA protein. TaGamyb binds the AACA motif and activates the GluD1‐2 gene promoter in Arabidopsis (Guo et al ., ). Near the GLM‐GATA box, AACA and RY motifs were identified (Ravel et al ., ).…”

Section: Discussionmentioning

confidence: 97%

The bZIP transcription factor SPA Heterodimerizing Protein represses glutenin synthesis in Triticum aestivum

et al. 2019

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The quality of wheat grain is mainly determined by the quantity and composition of its grain storage proteins (GSPs). Grain storage proteins consist of low-and high-molecular-weight glutenins (LMW-GS and HMW-GS, respectively) and gliadins. The synthesis of these proteins is essentially regulated at the transcriptional level and by the availability of nitrogen and sulfur. The regulation network has been extensively studied in barley where BLZ1 and BLZ2, members of the basic leucine zipper (bZIP) family, activate the synthesis of hordeins. To date, in wheat, only the ortholog of BLZ2, Storage Protein Activator (SPA), has been identified as playing a major role in the regulation of GSP synthesis. Here, the ortholog of BLZ1, named SPA Heterodimerizing Protein (SHP), was identified and its involvement in the transcriptional regulation of the genes coding for GSPs was analyzed. In gel mobility shift assays, SHP binds cis-motifs known to bind to bZIP family transcription factors in HMW-GS and LMW-GS promoters. Moreover, we showed by transient expression assays in wheat endosperm that SHP acts as a repressor of the activity of these gene promoters. This result was confirmed in transgenic lines overexpressing SHP, which were grown with low and high nitrogen supply. The phenotype of SHP-overexpressing lines showed a lower quantity of both LMW-GS and HMW-GS, while the quantity of gliadin was unchanged, whatever the nitrogen availability. Thus, the gliadin/glutenin ratio was increased, which suggests that gliadin and glutenin genes may be differently regulated.

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Section: Discussionmentioning

confidence: 97%

The bZIP transcription factor SPA Heterodimerizing Protein represses glutenin synthesis in Triticum aestivum

et al. 2019

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“…[30][31][32] In wheat, the number of wheat MYB TFBSs is correlated with the abundance of glutenin in different wheat cultivars. 15) In the present study, we predicted the TFBSs in the upstream regions of wheat fl-cDNA sequences using PWMs; the profiles of 14 TFBS types were analyzed with 54 PWMs. The Pvalue of the two-tailed test was 1.000, indicating that there were no significant differences among the average numbers of TFBSs per fl-cDNA for the 14 TFBS types ( Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…48) Moreover, Guo et al found that MYB TFs play an important role in the regulation of the glutenin gene expression during wheat endosperm development by directly binding to the promoter of TaGLU-1 and by recruiting GCN5 to modulate histone acetylation. 15) The plant B3 superfamily has two subfamilies, ARF and B3, and the B3 domain is the defining domain. 12) The B3 family is involved in flowering time control and in organ growth and polarity.…”

Section: Discussionmentioning

confidence: 99%

“…Guo et al found that the numbers of TaGAMyb binding sites were correlated with the abundance of high-molecular-weight glutenin subunit (HMW-GS) proteins, through binding of the promoters of wheat HMW-GS genes. 15) The regulation mechanisms and relationships between TFs and their TFBSs have been experimentally confirmed in many plants. [16][17][18][19] However, studies on the functions and molecular mechanisms of TFBSs can be time-consuming and labor-intensive if there is no prior information.…”

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confidence: 99%

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Identification and positional distribution analysis of transcription factor binding sites for genes from the wheat fl-cDNA sequences

Chen

Guo

Chen

et al. 2017

Bioscience, Biotechnology, and Biochemistry

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The binding sites of transcription factors (TFs) in upstream DNA regions are called transcription factor binding sites (TFBSs). TFBSs are important elements for regulating gene expression. To date, there have been few studies on the profiles of TFBSs in plants. In total, 4,873 sequences with 5' upstream regions from 8530 wheat fl-cDNA sequences were used to predict TFBSs. We found 4572 TFBSs for the MADS TF family, which was twice as many as for bHLH (1951), B3 (1951), HB superfamily (1914), ERF (1820), and AP2/ERF (1725) TFs, and was approximately four times higher than the remaining TFBS types. The percentage of TFBSs and TF members showed a distinct distribution in different tissues. Overall, the distribution of TFBSs in the upstream regions of wheat fl-cDNA sequences had significant difference. Meanwhile, high frequencies of some types of TFBSs were found in specific regions in the upstream sequences. Both TFs and fl-cDNA with TFBSs predicted in the same tissues exhibited specific distribution preferences for regulating gene expression. The tissue-specific analysis of TFs and fl-cDNA with TFBSs provides useful information for functional research, and can be used to identify relationships between tissue-specific TFs and fl-cDNA with TFBSs. Moreover, the positional distribution of TFBSs indicates that some types of wheat TFBS have different positional distribution preferences in the upstream regions of genes.

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“…In wheat, TaSPA, the O2-like bZIP factor, recognizes the GLM (GCN4-like motif) in the bi-factorial endosperm box to regulate expression of the low molecular weight glutenin subunit (LMW-GS) gene (Albani et al, 1997). TaGAMyb, which attaches to the R2R3MYB family, has been identified to interact with the histone acetyltransferase TaGCN5 to activate the expression of TaGLU-1Dy , a HMW-GS gene in wheat (Guo et al, 2015). Regulation of the expression of SSPs is accomplished by the integrated action of multitudinous TFs (Shekhar et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization of TaFUSCA3, a B3-Superfamily Transcription Factor Gene in the Wheat

et al. 2017

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The end-use quality of wheat, including its unique rheology and viscoelastic properties, is predominantly determined by the composition and concentration of gluten proteins. While, the mechanism regulating expression of the seed storage protein (SSP) genes and other related genes in wheat remains unclear. In this study, we report on the cloning and functional identification of TaFUSCA3, a B3-superfamily transcription factor (TF) gene in wheat. Sequence alignment indicated that wheat and barley FUSCA3 genes are highly conserved. Quantitative reverse-transcription (qRT)-PCR analysis showed that the transcript of TaFUSCA3 was accumulated mostly in the stamens and the endosperms of immature wheat seeds. Yeast-one-hybrid results proved that the full-length TaFUSCA3 and its C-terminal region had transcriptional activities. Yeast-two-hybrid and bimolecular fluorescence complementation assays indicated that TaFUSCA3 could activate the expression of the high molecular weight glutenin subunit gene Glu-1Bx7 and interact with the seed-specific bZIP protein TaSPA. DNA-protein-interaction enzyme-linked immunosorbent assay demonstrated that TaFUSCA3 specifically recognizes the RY-box of the Glu-1Bx7 promoter region. Transient expression results showed that TaFUSCA3 could trans-activate the Glu-1Bx7 promoter, which contains eight RY-box sequences. TaFUSCA3 was unable to activate the downstream transcription when the RY-box was fully mutated. TaFUSCA3 could activate the transcription of the At2S3 gene promoter in a complementation of loss-of-function experiment using the Arabidopsis thaliana line fus3-3, which is a FUSCA3 mutant, demonstrating the evolutionary conservation of the TaFUSCA3 gene. In conclusion, the wheat B3-type TF, TaFUSCA3, is functional conserved between monocot and dicot, and could regulate SSP gene expression by interacting specifically with TaSPA.

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The wheat transcription factor TaGAMyb recruits histone acetyltransferase and activates the expression of a high‐molecular‐weight glutenin subunit gene

Cited by 53 publications

References 43 publications

The bZIP transcription factor SPA Heterodimerizing Protein represses glutenin synthesis in Triticum aestivum

The bZIP transcription factor SPA Heterodimerizing Protein represses glutenin synthesis in Triticum aestivum

Identification and positional distribution analysis of transcription factor binding sites for genes from the wheat fl-cDNA sequences

Functional Characterization of TaFUSCA3, a B3-Superfamily Transcription Factor Gene in the Wheat

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