Genetic Study of Cryptosporidium with SSU-rRNA in Children Younger Than Ten Referring to Hospitals of Zabol, Southeast of Iran

Abstract

Background: Cryptosporidium parasite is the cause of human gastroenteritis and other cold and warm-blooded animals that have been widely distributed throughout the world. Genetic information on opportunistic pathogens in immunocompromised patients leads to an increase in the information on epidemiology, patient care, patient management, and rescue. In Iran, infection to Cryptosporidium spp. has been reported, yet only molecular genes can differentiate species and genotype discrimination of the cyst. The molecular assays indicated that Cryptosporidium parvum is the most common species found in Iran, followed by C. hominis. Objectives: The present study aimed at determining the genetic diversity of Cryptosporidium (C.) in children with diarrhea using the PCR-RFLP method and SSU gene. Methods: In this study, stool specimens were collected from 182 children with diarrhea referring to Zabol hospitals. Slides and shitter procedure were done and Ziehl-Neelsen stain was observed directly; an examination was made to identify the parasite, and PCR-RFLP were eventually performed on DNA extracted from the isolates. Results: Out of 182 stool specimens, 27 isolates were identified as Cryptosporidium, using Ziehl-Neelsen stain method, of which 17 and 10 isolates were respectively reported to be C. parvum and C. hominis after the molecular examination. Conclusions: Both human and cattle genotypes are seen in children with diarrhea, yet since the dominant species is C. parvum, zoonosis is more common than human transmission and human-livestock contact is considered as the most important source of human contamination.

Genotype and subtype analyses of Cryptosporidium isolate from humans by gp60 PCR-RLFP in Zabol, Southeast of Iran

“…This study has an ethical code zbmu.1.REC.1395.45 issued by the ethics committee of Zabol University of Medical science. This study is a continuation of our work on SSUrRNA that was published before in Shiraz E-Medical Journal 20 (16).…”

Prevalence and Molecular Genotyping of Cryptosporidium Spp. in Diarrheic Patients from Bandar Abbas City, Southern Iran

“…As we can see, all three women are housewives and likely to be infected with dirty vegetables. Most of the infections in Iran are C. parvum (26)(27)(28). Molecular studies in the Middle East countries showed C. parvum, as the most dominant species in human infections (22) this is contrary to study of Squire and Ryan, which shows that C. hominis is the most cases of infection in Africa (27), detected C. hominis while none of the isolates in the present study were C. hominis.…”

“…Most of the infections in Iran are C. parvum (26)(27)(28). Molecular studies in the Middle East countries showed C. parvum, as the most dominant species in human infections (22) this is contrary to study of Squire and Ryan, which shows that C. hominis is the most cases of infection in Africa (27), detected C. hominis while none of the isolates in the present study were C. hominis. In contrary to our study, the other species of Cryptosporidium, C. meleagridis is one of the major human parasitic pathogens in African countries (29).…”

Diagnóstico E Identificación De Especies De Cryptosporidium en Población Infantil De Areas Rurales en Panamá.

“…A nivel mundial, existen diversos estudios en donde ha sido posible la caracterización de Cryptosporidium a nivel de especie 15,24,32,[33][34][35] . No obstante, hasta donde conocemos, este es el primer reporte de las especies de Cryptosporidium circulantes en niños menores de cinco años de comunidades rurales en Panamá.…”