Genetic studies of the role of fatty acid and coenzyme A in photocatalytic inactivation of Escherichia coli

Differences in photoelectrocatalytic inactivation processes between E. coli and its isogenic single gene knockoff mutants: Destruction of membrane framework or associated proteins?

“…Therefore, the parental strain E. coli BW25113 with a higher SFA ratio was more resistant to PEC inactivation compared with the mutants. This conclusion also confirms other research that found that the higher the UFA to SFA ratio, the more sensitive the bacteria are to PC inactivation [10,29]. These results suggest that UFA was likely the important initial target of RSs during PC and PEC process, leading to the structural destruction of bacterial membrane framework.…”

“…For Gram negative bacteria, the outer membrane, cell wall, and cytoplasmic membrane are located at the outside of the cell, and thus are the most probable targets of attack. The damaged bacterial envelopes seen in SEM (scanning electron microscopic) and TEM (transmission electron microscopic) images of PC and PEC treated samples support this hypothesis [9][10][11][12].…”

Comparative study on the photoelectrocatalytic inactivation of Escherichia coli K-12 and its mutant Escherichia coli BW25113 using TiO2 nanotubes as a photoanode

“…The different susceptibilities and responses of the two strains toward external stress, in this case the PC or PEC treatment, is probably due to the different genes possessed [30,38]. For the mutant E. coli BW25113, lacZ, araBAD and rhaBAD were knocked out from E. coli K-12 [38].…”

“…To date, the contributions of different RSs to bacterial inactivation remain controversial. The majority of recent studies have primarily focused on the PC inactivation efficiency of a single bacterial strain, while comparative studies on PC inactivation of a series of closely related bacterial mutant strains are rarely performed [30]. Nevertheless, to date, a comparative study of PEC inactivation of different mutants using highly ordered TiO 2 nanotubular photoanode has not been attempted yet.…”

Unveiling the photoelectrocatalytic inactivation mechanism of Escherichia coli : Convincing evidence from responses of parent and anti-oxidation single gene knockout mutants

“…The gene katG is part of the H 2 O 2 dependent oxyR regulated response system, while the gene katE is regulated by RpoS independent of H 2 O 2 induction (Mukhopadhyay and Schellhorn, 1994;Chiang and Schellhorn, 2012 (Nachin et al, 2005). However, only a few studies have applied this method to investigate PC inactivation mechanisms (Gao et al, 2012). Therefore, this study investigates PC and PEC inactivation mechanisms using E. coli BW25113 as a parental strain, and its isogenic mutants with the single gene katG (E. coli JW3914-1) or sodA (E. coli JW3879-1) deleted.…”