Unveiling the photoelectrocatalytic inactivation mechanism of Escherichia coli : Convincing evidence from responses of parent and anti-oxidation single gene knockout mutants

Sun, Hongwei; Li, Guiying; An, Taicheng; Zhao, Huijun; Wong, Po Keung

doi:10.1016/j.watres.2015.10.003

Cited by 50 publications

(22 citation statements)

References 40 publications

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“…2. This lag phase might be due to the protection of the cell wall and the defense of antioxidative enzymes against oxidative stress (Sun et al, 2016). After 90 min of electrolysis, the removal efficiency reached 1.92 logs for sul1, 1.74 logs for sul2, 2.63 logs for tetM, 2.35 logs for tetW, and 1.82 logs for 16S rRNA, which was lower than that in the EC process.…”

Section: Ef Processmentioning

confidence: 83%

Removal of Intracellular and Extracellular Antibiotic Resistance Genes from Swine Wastewater by Sequential Electrocoagulation and Electro-Fenton Processes

Chen

Gao

Lou

et al. 2021

Environmental Engineering Science

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Antibiotics are often used excessively in the livestock industry, and the effluent from livestock wastewater treatment is one of the main sources of antibiotic resistance genes (ARGs) in the environment. Biological treatments and traditional disinfection cannot effectively remove ARGs from this wastewater. This study evaluated the feasibility of removing ARGs from actual swine wastewater treatment effluent using single and sequential electrocoagulation (EC) and electro-Fenton (EF) processes. An EC process with 60 min of electrolysis removed the selected intracellular ARGs with a removal efficiency of 2.21-2.83 logs and the extracellular ARGs with a removal efficiency of 1.82-2.56 logs. In comparison, the EF process was less efficient for the removal of intracellular ARGs (1.03-2.19 logs), but more efficient for the removal of extracellular ARGs (3.19-4.02 logs). Compared to the individual EC and EF processes alone, the sequential EC/EF processes were more effective for the removal of both intracellular and extracellular ARGs, with 2.49-3.25 logs and 3.23-4.38 logs of removal efficiency, respectively. Our results show that the sequential EC/EF treatment might be a promising technology for removing both intracellular and extracellular ARGs from swine wastewater.

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Section: Ef Processmentioning

confidence: 83%

Removal of Intracellular and Extracellular Antibiotic Resistance Genes from Swine Wastewater by Sequential Electrocoagulation and Electro-Fenton Processes

Chen

Gao

Lou

et al. 2021

Environmental Engineering Science

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“…Refer to the Total Antioxidant Capacity Assay Kit with FRAP method (Cat#S0116; Beyotime Institute of Biotechnology, Shanghai, China) to detect the total antioxidant capacity [ 53 ], the Total Superoxide Dismutase Assay Kit with WST-8 (Cat#S0101M; Beyotime Institute of Biotechnology, Shanghai, China) to detect superoxide dismutase activity and the Catalase Assay Kit (Cat#S0051; Beyotime Institute of Biotechnology, Shanghai, China) to detect catalase [ 54 , 55 ]. Refer to the Lactate Dehydrogenase Activity Assay Kit (Cat#MAK066-KT; Sigma-Aldrich, Lenexa, KS, USA) to detect lactate dehydrogenase activity and refer to Lipid Peroxidation MDA Assay Kit (Cat#S0131S; Beyotime Institute of Biotechnology, Shanghai, China) to detect malondialdehyde [ 53 , 56 ].…”

Section: Methodsmentioning

confidence: 99%

Modular Assembly of Ordered Hydrophilic Proteins Improve Salinity Tolerance in Escherichia coli

Guo

Zhao

Tang

et al. 2021

IJMS

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Most late embryogenesis abundant group 3 (G3LEA) proteins are highly hydrophilic and disordered, which can be transformed into ordered α-helices to play an important role in responding to diverse stresses in numerous organisms. Unlike most G3LEA proteins, DosH derived from Dinococcus radiodurans is a naturally ordered G3LEA protein, and previous studies have found that the N-terminal domain (position 1–103) of DosH protein is the key region for its folding into an ordered secondary structure. Synthetic biology provides the possibility for artificial assembling ordered G3LEA proteins or their analogues. In this report, we used the N-terminal domain of DosH protein as module A (named DS) and the hydrophilic domains (DrHD, BnHD, CeHD, and YlHD) of G3LEA protein from different sources as module B, and artificially assembled four non-natural hydrophilic proteins, named DS + DrHD, DS + BnHD, DS + CeHD, and DS + YlHD, respectively. Circular dichroism showed that the four hydrophile proteins were highly ordered proteins, in which the α-helix contents were DS + DrHD (56.1%), DS + BnHD (53.7%), DS + CeHD (49.1%), and DS + YLHD (64.6%), respectively. Phenotypic analysis showed that the survival rate of recombinant Escherichia coli containing ordered hydrophilic protein was more than 10% after 4 h treatment with 1.5 M NaCl, which was much higher than that of the control group. Meanwhile, in vivo enzyme activity results showed that they had higher activities of superoxide dismutase, catalase, lactate dehydrogenase and less malondialdehyde production. Based on these results, the N-terminal domain of DosH protein can be applied in synthetic biology due to the fact that it can change the order of hydrophilic domains, thus increasing stress resistance.

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“…Intracellular ROS were responsible for the oxidative damage of enzymes and DNA, which will lead to the inactivation of bacteria ( Rahmanto et al, 2012 ). Thus, the intracellular ROS (mainly HO • and H 2 O 2 ) level in the simulated sunlight treated AR E. coli was determined with 2 ,7 -dichlorodihydrofluorescein diacetate (DCFH-DA) as a fluorescent probe ( Sun et al, 2014( Sun et al, , 2016. The detailed method is described in Text S7 in the SI.…”

Section: Investigation Of the Inactivation Mechanismsmentioning

confidence: 99%

Simulated sunlight-induced inactivation of tetracycline resistant bacteria and effects of dissolved organic matter

Zhang

Liu

et al. 2020

Water Research

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The transmission of antibiotic resistance in surface water has attracted much attention due to its increasing threat to human health. The role of sunlight irradiation and the effect of dissolved organic matter (DOM) on the transmission of antibiotic resistance are still unclear. In this study, photo-inactivation of antibiotic resistant bacteria (ARB) was investigated using antibiotic resistant E. coli (AR E. coli ) that contained the tetracycline resistance gene (Tc-ARG) as a representative. The results showed that AR E. coli underwent significant photo-inactivation due to the membrane damage induced by direct irradiation and by the generated reactive oxygen species. Simulated sunlight irradiation specifically suppressed the expression of tetracycline resistance, which is attributed to the destruction of tetracycline-specific efflux pump. Tetracycline inhibited the photo-inactivation of AR E. coli due to its selective pressure on tetracycline resistant E. coli and competitive light absorption effect. Suwannee River fulvic acid (SRFA), a representative DOM, promoted the inactivation of AR E. coli and further inhibited the expression of tetracycline resistance gene due to the generation of its excited triplet state, singlet oxygen, and hydroxyl radical. The extracellular Tc-ARG also underwent fast photodegradation under light irradiation and in the presence of SRFA, which leads to the decrease of its transformation efficiency. This study provided insight into the sunlight-induced inactivation of ARB, which is of significance for understanding the transmission of tetracycline resistance in surface water.

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Unveiling the photoelectrocatalytic inactivation mechanism of Escherichia coli : Convincing evidence from responses of parent and anti-oxidation single gene knockout mutants

Cited by 50 publications

References 40 publications

Removal of Intracellular and Extracellular Antibiotic Resistance Genes from Swine Wastewater by Sequential Electrocoagulation and Electro-Fenton Processes

Removal of Intracellular and Extracellular Antibiotic Resistance Genes from Swine Wastewater by Sequential Electrocoagulation and Electro-Fenton Processes

Modular Assembly of Ordered Hydrophilic Proteins Improve Salinity Tolerance in Escherichia coli

Simulated sunlight-induced inactivation of tetracycline resistant bacteria and effects of dissolved organic matter

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