Genetic structure and relationships within and between cultivated and wild sorghum (Sorghum bicolor (L.) Moench) in Kenya as revealed by microsatellite markers

Mutegi, Evans; Sagnard, Fabrice; Semagn, Kassa; Deu, Monique; Muraya, Moses M.; Kanyenji, Ben M.; Villiers, Santie de; Kiambi, Dan; Herselman, L.; Labuschagne, Maryke

doi:10.1007/s00122-010-1504-5

Cited by 60 publications

(68 citation statements)

References 67 publications

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“…Accessions from South Sudan with rich private alleles include 'Jeri', 'Medenga', 'Okabir', 'Deri', 'Kodu Kine' and 'Oderi'. The mean gene diversity (H e =0.50) for the current sorghum populations is slightly lower than the value estimated for cultivated sorghum of Kenya (0.59) by Mutegi et al, (2011). The H o values were generally lower than the H e values, indicating deviations from the random mating and low cross pollination rate due to the isolation among the different accessions of each Molecular Plant Breeding 2014, Vol.5, No.13, 1-12 http://mpb.biopublisher.ca population or among the diverse geographical sampling.…”

Section: Discussioncontrasting

confidence: 65%

Genetic Diversity Analysis of Eritrean Sorghum (<i>Sorghum bicolor</i> (L.) Moench) Germplasm using SSR Markers

Tesfamichael¹

2014

mpb

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Eritrea is considered a center of origin for sorghum, the main cereal crop in terms of area under cultivation and production in the country. There have been very little genetic diversity studies done on the Eritrean sorghum before. To improve this crop, the knowledge of genetic diversity estimation is needed on the available germplasm. The aim of this study was therefore to asses the extent of genetic diversity within and among 98 sorghum genotypes collected from Eritrea alongside 42 regional reference accessions from the International Crop Research Institute for Semi-Arid Tropics (ICRISAT) using a set of 29 Simple Sequence Repeat (SSR) markers. An average of 4.8 alleles per marker was recorded. The mean polymorphism information content (PIC) value for the SSR loci was 0.52. The Analysis of Molecular Variation (AMOVA) revealed that 12% of the variation resulted from the difference among populations, 31% within individual populations and 57% among individual accessions of the sub populations. Neighbor joining phylogeny tree based on genetic similarity coefficient revealed three distinct groups of clustering with the Eritrean populations further sub clustered into three groups. The Eritrean sorghum accessions from Gash Barka and South regions and South Sudan accessions recorded the highest private alleles. The results of Principal Coordinate Analysis (PCoA) also classified the sorghum accessions into three major groups. Genetic distance matrix revealed that the Eritrean accessions are more related to each other compared to the regional accessions. The existence of higher level of allelic richness, close genetic distance and an isolated clustering of the Eritrean population indicates that the accessions have not been introgressed with foreign genes and are valuable resource for future breeding programs of this crop.

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Section: Discussioncontrasting

confidence: 65%

Genetic Diversity Analysis of Eritrean Sorghum (<i>Sorghum bicolor</i> (L.) Moench) Germplasm using SSR Markers

Tesfamichael¹

2014

mpb

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“…The main evolutionary forces shaping the genetic diversity in the populations of cultivated plants are among others gene flows, selection in connection with environmental heterogeneity and/or preference criteria of farmers-consumers and genetic variation due to randomness (genetic drift) (Neal, 2004;Mutegi et al, 2011). In the present work, the first factor of the structuration of sorghum genetic diversity identified was the racial parameter.…”

Section: Population Structure and Distributionmentioning

confidence: 62%

Genetic diversity of Sorghum bicolor (L.) Moench landraces from Northwestern Benin as revealed by microsatellite markers

Missihoun¹,

Adoukonou‐Sagbadja²,

Sédah³

et al. 2015

Afr. J. Biotechnol.

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The understanding of genetic diversity within local crop varieties constitutes an important step in the preservation of their genetic potential. The objective of this study was to assess the genetic diversity of sorghum (Sorghum bicolor (L.) Moench) cultivated in the Northwest of Benin and to reveal certain fundamental evolutionary mechanisms. A total of 61 accessions of sorghum landraces belonging to the four identified races in Benin were estimated using 20 microsatellite markers. For all the loci analyzed, 140 polymorphic alleles were detected with a mean value of 7.00 per locus and polymorphic information content (PIC) average value was 0.33 for all the 20 simple sequence repeats (SSRs), suggesting an important genetic diversity within the cultivated sorghum germplasm used. An unweighted pair group method arithmetic average (UPGMA) clustering and principal coordinate analysis (PCoA) based on DICE coefficient revealed three major genetic groups supported by two main components: the botanical race and the morpho-physiological characteristics of the grains (colour and degree of bitterness). It was thus recommended that further research on genetic diversity of sorghum should integrate these genetic parameters for a better preservation of the genetic resources of this important crop in Benin.

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“…All subspecies of S. bicolor are inter-fertile under sympatric conditions, leading to a continuum of wilddomesticate complex forms that have been documented to occur in many sorghum growing parts of Africa (Mutegi et al, 2010;Tesso et al, 2008;Dogget, 1988;Dogget and Majisu, 1968). Therefore, a wide genetic diversity is expected in the landraces of cultivated sorghum in Africa.…”

Section: Introductionmentioning

confidence: 99%

“…In lower Eastern Kenya, a wide diversity of sorghum landraces is cultivated under diverse agro-climatic conditions and practices by subsistence farmers (Muui et al, 2013;Mutegi et al, 2010). Farmers maintain sorghum landraces unique in their adaptation, grain yield, quality of harvested products, biotic stress resistance and in postharvest processing (Muui et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Genetic variability of sorghum landraces from lower Eastern Kenya based on simple sequence repeats (SSRs) markers

Muui¹,

Muasya²,

Kirubi³

et al. 2016

Afr. J. Biotechnol.

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The aim of this study was to estimate the genetic variability of sorghum landraces grown in lower eastern Kenya based on simple sequence repeats (SSRs) markers. A total of 44 landraces obtained from the farmers and four improved varieties were analyzed using 20 SSR markers. All markers were polymorphic with polymorphism information content (PIC) value ranging from 0.04 to 0.81 with a mean of 0.49. Heterozygosity ranged from 0.00 to 0.04 suggesting that each detected a single genetic locus and that each of the sorghum accession used was stable. The alleles ranged between 2 and 10 and an average of 5.05 alleles per primer pair. The gene diversity ranged from 0.04 to 0.83 with a mean value of 0.53. All possible allele combinations ranged from 2 to 10, while major allele frequency ranged from 0.32 to 0.98. Genetic distances varied from 0.15 to 0.90 with two genotypes Karuge 1 and Karuge 2 obtained from Kiritiri in Mbeere having the minimum (0.15) and indication of very close relatedness. The diversity of the landraces was structured more on geographical locations and on seed colorations than agroecological conditions. Such intraregional genetic proximity in sorghum landraces would arise through seed exchanges among farmers. Analysis of molecular variation indicated higher variation within populations than among the groups. The genetic diversity can be exploited in hybridization programs to improve sorghum varieties grown by farmers in semi arid areas.

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Genetic structure and relationships within and between cultivated and wild sorghum (Sorghum bicolor (L.) Moench) in Kenya as revealed by microsatellite markers

Cited by 60 publications

References 67 publications

Genetic Diversity Analysis of Eritrean Sorghum (<i>Sorghum bicolor</i> (L.) Moench) Germplasm using SSR Markers

Genetic Diversity Analysis of Eritrean Sorghum (<i>Sorghum bicolor</i> (L.) Moench) Germplasm using SSR Markers

Genetic diversity of Sorghum bicolor (L.) Moench landraces from Northwestern Benin as revealed by microsatellite markers

Genetic variability of sorghum landraces from lower Eastern Kenya based on simple sequence repeats (SSRs) markers

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