Genetic similarity of Theobroma cacao L. accessions maintained in duplicate at the cacao research center germplasm collection based on RAPD markers

Faleiro, Fábio Gelape; Yamada, Milton Macoto; Lopes, Uilson Vanderlei; Gelape, Alessandra Silva; Cássia, Rita de; Bahia, Siqueira; Gomes, Luana Mahé Costa; Santos, Ronaldo Carvalho; Figueiredo, Reinaldo

doi:10.12702/1984-7033.v02n03a15

“…Genomic DNA was extracted from leaf tissue using the cetyltrimethyl ammonium bromide method (Doyle & Doyle, 1990), as modified by Faleiro et al (2002). The quantity and quality of the DNA was checked by using a Cole-Parmer Jenway Genova Nano Micro-volume Life Science spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA, USA), and the DNA size range and degree of fragmentation was checked by an electrophoretic separation of an aliquot through a 0.8% agarose gel containing 1Â Tris-Borate-Ethylene diamine tetra acetic acid buffer and ethidium bromide.…”

Section: Extraction Of Genomic Dnamentioning

confidence: 99%

Characterization of resistance to angular leaf spot of common bean (Phaseolus vulgaris L.) breeding line SPS50HB

Firew,

Rezene,

Demissie

2023

Plant Breeding

0

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The common bean (Phaseolus vulgaris L.) makes an important contribution to the human diet, particularly in Africa and Latin America. Because angular leaf spot (ALS), caused by the fungal pathogen Pseudocercospora griseola, is one of the most severe foliar diseases of the bean plant, an important priority is to identify genes encoding resistance. The present study focused on the resistance shown by the Mesoamerican common bean breeding line SPS50HB. From the pattern of segregation for resistance displayed in an F2 population bred from a cross between SPS50HB and the ALS‐susceptible Ethiopian variety Red Wolaita, it was concluded that the resistance of SPS50HB is controlled by two unlinked dominant genes. An allelism test indicated that one of these genes was either identical with, allelic to, or closely linked to the major gene Phg‐2 carried by variety Mexico 54. The sequence‐characterized amplified region assays OPEO4 and PF13, which are diagnostic for an ALS resistance gene carried by the germplasm accession G10909, both tracked a possible second gene present in SPS50HB.

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“…Prosedur RAPD lebih murah, lebih cepat, membutuhkan sampel DNA dalam jumlah lebih sedikit (0,5-50 ng), tidak memerlukan radioisotop, dan tidak terlalu membutuhkan keahlian untuk pelaksanaannya dibanding penanda lainnya seperti RFLP. Teknik RAPD telah banyak diaplikasikan dalam analisis keragaman genetik tanaman perkebunan, di antaranya kelapa sawit (Toruan-Mathius et al, 2001), kakao (Faleiro et al, 2002) dan karet (Nurhaimi-Haris et al, 1998). Selain itu, teknik RAPD telah digunakan dalam beberapa penelitian analisis genetik mikroorganisme, di antaranya keragaman genetik Staphylococcus aureus (Morandi et al, 2010).…”

Section: Pendahuluanunclassified

Analisis keragaman genetik Ganoderma spp. yang berasosiasi dengan tanaman kakao dan tanaman pelindungnya menggunakan Random Amplified Polymorphic DNA (RAPD) Genetic diversity analysis of Ganoderma spp. associated with cocoa and its shade trees using Random Amplified Polymorphic DNA (RAPD)

Minarsih¹,

Lingga²,

Darmono³

et al. 2016

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Information on genetic diversity of Ganoderma spp.causing root rot disease in crops is important to developa proper strategy for the control of Ganoderma disease. Theobjectives of this research were to study the genetic diversityof Ganoderma spp. associated with cacao and its shade trees(Albazia faltacaria, Swietenia mahogani, Adenatheramicrosperma and Leucaena leucocephala) by randomamplified polymorphic DNA (RAPD) analysis. Fourty fivesamples of Ganoderma spp. were used in this research. Theresults showed that DNA amplification using 10 arbitraryoligonucleotide primers produced 220 DNA fragmentsshowing polymorphisms. The cluster analysis showed that 45number of Ganoderma samples had a high variability with thecoefficient value ranged from 0.71 to 0.91. Further analysisusing Winboot software showed that three groups ofGanoderma spp. had a high degree of confidence (>50 %),which were Ganoderma samples from sengon (Paraserianthessp.) of Tasikmalaya, sengon (Paraserianthes sp.) ofPalembang, and mahogany of Jember; whereas the othergroups of samples had a low degree of confidence (<50%).AbstrakInformasi tentang keragaman genetik Ganoderma spp.sebagai penyebab penyakit busuk akar pada tanamanperkebunan sangat diperlukan untuk menerapkan strategiyang tepat dalam upaya perlindungan tanaman perkebunan.Penelitian ini bertujuan untuk mengetahui keragaman genetikGanoderma spp. yang berasosiasi dengan tanaman kakao dantanaman pelindungnya (sengon, mahoni, saga dan lamtoro)dari berbagai wilayah di Indonesia menggunakan penandamolekuler random amplified polymorphic DNA (RAPD).Sebanyak 45 sampel Ganoderma spp digunakan dalampenelitian ini. Amplifikasi DNA dengan 10 primer terpilihmenghasilkan 220 fragmen DNA yang menunjukkan adanyapolimorfisme. Hasil analisis menunjukkan adanya keragamanyang cukup tinggi di antara sampel Ganoderma spp. daripohon inang dan wilayah yang berbeda, dengan nilaikoefisien 0,71-0,91. Berdasarkan analisis bootstrapdiketahui bahwa tiga kelompok sampel Ganoderma spp.memiliki tingkat kepercayaan yang tinggi (>50 %) yaitukelompok Ganoderma spp. yang berasosiasi dengan pohonsengon asal Tasikmalaya, sengon Palembang, dan mahoniJember; sedangkan pengelompokan lainnya menunjukkmenunjukkan tingkat kepercayaan yang rendah (<50 %).

show abstract

“…Prosedur RAPD lebih murah, lebih cepat, membutuhkan sampel DNA dalam jumlah lebih sedikit (0,5-50 ng), tidak memerlukan radioisotop, dan tidak terlalu membutuhkan keahlian untuk pelaksanaannya dibanding penanda lainnya seperti RFLP. Teknik RAPD telah banyak diaplikasikan dalam analisis keragaman genetik tanaman perkebunan, di antaranya kelapa sawit (Toruan-Mathius et al, 2001), kakao (Faleiro et al, 2002) dan karet (Nurhaimi-Haris et al, 1998). Selain itu, teknik RAPD telah digunakan dalam beberapa penelitian analisis genetik mikroorganisme, di antaranya keragaman genetik Staphylococcus aureus (Morandi et al, 2010).…”

Section: Pendahuluanunclassified

Analisis keragaman genetik Ganoderma spp. yang berasosiasi dengan tanaman kakao dan tanaman pelindungnya menggunakan Random Amplified Polymorphic DNA (RAPD) Genetic diversity analysis of Ganoderma spp. associated with cocoa and its shade trees using Random Amplified Polymorphic DNA (RAPD)

MINARSIH¹,

LINGGA²,

DARMONO³

et al. 2016

MP

0

View full text Add to dashboard Cite

Information on genetic diversity of Ganoderma spp.causing root rot disease in crops is important to developa proper strategy for the control of Ganoderma disease. Theobjectives of this research were to study the genetic diversityof Ganoderma spp. associated with cacao and its shade trees(Albazia faltacaria, Swietenia mahogani, Adenatheramicrosperma and Leucaena leucocephala) by randomamplified polymorphic DNA (RAPD) analysis. Fourty fivesamples of Ganoderma spp. were used in this research. Theresults showed that DNA amplification using 10 arbitraryoligonucleotide primers produced 220 DNA fragmentsshowing polymorphisms. The cluster analysis showed that 45number of Ganoderma samples had a high variability with thecoefficient value ranged from 0.71 to 0.91. Further analysisusing Winboot software showed that three groups ofGanoderma spp. had a high degree of confidence (>50 %),which were Ganoderma samples from sengon (Paraserianthessp.) of Tasikmalaya, sengon (Paraserianthes sp.) ofPalembang, and mahogany of Jember; whereas the othergroups of samples had a low degree of confidence (<50%).AbstrakInformasi tentang keragaman genetik Ganoderma spp.sebagai penyebab penyakit busuk akar pada tanamanperkebunan sangat diperlukan untuk menerapkan strategiyang tepat dalam upaya perlindungan tanaman perkebunan.Penelitian ini bertujuan untuk mengetahui keragaman genetikGanoderma spp. yang berasosiasi dengan tanaman kakao dantanaman pelindungnya (sengon, mahoni, saga dan lamtoro)dari berbagai wilayah di Indonesia menggunakan penandamolekuler random amplified polymorphic DNA (RAPD).Sebanyak 45 sampel Ganoderma spp digunakan dalampenelitian ini. Amplifikasi DNA dengan 10 primer terpilihmenghasilkan 220 fragmen DNA yang menunjukkan adanyapolimorfisme. Hasil analisis menunjukkan adanya keragamanyang cukup tinggi di antara sampel Ganoderma spp. daripohon inang dan wilayah yang berbeda, dengan nilaikoefisien 0,71-0,91. Berdasarkan analisis bootstrapdiketahui bahwa tiga kelompok sampel Ganoderma spp.memiliki tingkat kepercayaan yang tinggi (>50 %) yaitukelompok Ganoderma spp. yang berasosiasi dengan pohonsengon asal Tasikmalaya, sengon Palembang, dan mahoniJember; sedangkan pengelompokan lainnya menunjukkmenunjukkan tingkat kepercayaan yang rendah (<50 %).

show abstract

Genetic similarity of Theobroma cacao L. accessions maintained in duplicate at the cacao research center germplasm collection based on RAPD markers

Cited by 8 publications

References 1 publication

Characterization of resistance to angular leaf spot of common bean (Phaseolus vulgaris L.) breeding line SPS50HB

Characterization of resistance to angular leaf spot of common bean (Phaseolus vulgaris L.) breeding line SPS50HB

Analisis keragaman genetik Ganoderma spp. yang berasosiasi dengan tanaman kakao dan tanaman pelindungnya menggunakan Random Amplified Polymorphic DNA (RAPD) Genetic diversity analysis of Ganoderma spp. associated with cocoa and its shade trees using Random Amplified Polymorphic DNA (RAPD)

Analisis keragaman genetik Ganoderma spp. yang berasosiasi dengan tanaman kakao dan tanaman pelindungnya menggunakan Random Amplified Polymorphic DNA (RAPD) Genetic diversity analysis of Ganoderma spp. associated with cocoa and its shade trees using Random Amplified Polymorphic DNA (RAPD)

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