Genetic Relationships between Loci Controlling Palmitic and Linolenic Acids in Soybean

Abstract

Three mutagen‐derived soybean [Glycine max (L.) Merr.] lines have been identified with unique levels of fatty acids. Levels of linolenic acid (18:3) in C1640 and palmitic acid (16:0) in C1726 C1727 are controlled by alleles with additive effects at three loci. If these loci are independent, soybean strains could be developed with unique and useful combinations of both linolenic and palmitic acids. The primary objective of this study was to determine the genetic relationships between loci controlling levels of palmitic acid and the locus controlling linolenic acid. Additionally, effects of altered levels of linolenic and palmitic acids on total fatty acid composition were determined. Fatty acid composition of F2 seeds from crosses of C1640 (low 18:3) with both C1726 (low 16:0) and C1727 (high 16:10) were determined by gas chromatography. Chi‐square analyses of the data demonstrated that the fan locus controlling low 18:3 was inherited independently from both the fap1 and fap2 loci controlling low and high levels of 16:0, respectively. There was no indication of an interaction among alleles at these loci. The decreases in palmitic acid due to fap1 and increases due to fap2 were associated primarily with changes in oleic and linoleic acids. Decreases in linolenic acid due to fan were associated primarily with increases in linoleic acid.

Relationships and inheritance of linolenic acid and seed lipoxygenases in soybean crosses designed to combine these traits

“…The LA content did show a strong negative correlation with linoleic acid content in both RO + OR and PO crosses and all the generations that were evaluated, similar to previous report for soybean by Nickell et al (1991). This was consistent with the decrease in relative 18:2D activity.…”

“…The phenotypic classes for chi- square analyses were obtained from breaks observed in the distributions of F 2 seeds for LA content and relative 18:2D activities (Nickell et al 1991). Seeds with LA content and relative 18:2D activities less than or greater than either parent were considered to be transgressive segregants.…”

Quantitative trait locus analysis of saturated fatty acids in a population of recombinant inbred lines of soybean

“…Previous studies on fatty acids were mainly aimed at improving the quality of soybean oil for human consumption, i.e., decreasing saturated and polyunsaturated fatty acids and increasing monounsaturated acid (Erickson et al 1988;Nickell et al 1991;Li et al 2002;Oliva et al 2006;Panthee et al 2006;Primomo et al 2002;Hyten et al 2004b;Cardinal et al 2007). Soybean lines with reduced palmitic acid or stearic acid content have been developed through mutagenesis, recurrent selection and hybridization (Erickson et al 1988;Bubeck et al 1989;Wilcox and Cavins 1990;Burton et al 1994).…”

Mapping the low palmitate fap1 mutation and validation of its effects in soybean oil and agronomic traits in three soybean populations

“…In addition, when lines homozygous for the fap 1 and fap 3 alleles were compared to normal lines derived from the same population, the low 16:0 lines have reduced 18:0, and increased 18:1, 18:2, and 18:3 contents (Ndzana et al 1994). Palmitate content in soybean oil was negatively correlated with 18:2 and positively correlated with 18:1 in a population that was segregating for the fap 1 and fan loci (Nickell et al 1991). In summary, homozygous lines carrying fap nc or both fap 1 and fap 3 alleles, not only have reduced 16:0 content but also have reduced 18:0 content and the effect on unsaturated fatty acid contents varies among populations.…”