Quantitative trait locus analysis of saturated fatty acids in a population of recombinant inbred lines of soybean

Quantitative trait locus analysis of unsaturated fatty acids in a recombinant inbred population of soybean

“…The same population derived from the cross SD02-4-59 9 A02-381100 for QTL analysis of saturated fatty acids (Wang et al 2012) was used for the analysis of unsaturated fatty acids. As discussed previously, the population was small, consisting of 87 F 5 -derived RILs, and the parents were not included due to lack or loss of seeds.…”

“…To diminish the impact of this defect and enhance the reliability of the results, the population was planted in multiple years and locations. In addition to the previous five tests (Wang et al 2012), the field experiments were also conducted again in two locations in 2011, and thus the phenotyping was performed in seven environments in total. Except for the experiments with single-row plots and one replication at Aurora, SD, USA in 2007 and2008 (designated as E07 andE08, respectively), all the RILs were planted in two-row plots with two replications in a randomized complete block design at Aurora, SD in 2009(E09AU and E10AU), Beresford, SD in 2009and 2011(E09BF and E11BF), and Volga, SD in 2011.…”

“…Based on the map we constructed (Wang et al 2012) and the preliminary results of QTL analysis of unsaturated fatty acids, 340 additional SSR markers were ordered and screened for polymorphism to narrow the putative intervals which showed associations with unsaturated fatty acids. In total, 516 polymorphic SNP markers and 477 polymorphic SSR markers were used to genotype the population.…”

“…Oil content in soybean is quantitatively inherited (Burton et al 1983;White et al 1961), while for fatty acids, in addition to minor genes (Bachlava et al 2009;Panthee et al 2006;Wang et al 2012), there may also be major genes regulating their inheritance (Bilyeu et al 2006;Bachlava et al 2009;Pham et al 2010). In some cases, single major genes or mutant alleles, either transgenic or non-transgenic, can dramatically increase oleic acid or significantly decrease linolenic acid (Bilyeu et al 2005;Pham et al 2010).…”

“…It is necessary to map and validate QTLs associated with unsaturated fatty acids in different environments and different genetic backgrounds to obtain a better understanding of the genetic basis of differences in fatty acids and to effectively use fatty acid germplasm resources. We have previously reported the QTLs associated with saturated fatty acids in a recombinant inbred population (Wang et al 2012). The QTLs for unsaturated fatty acids in the same population were also analyzed (data unpublished).…”