Genetic relationships among isolates of <i>Colletotrichum gloeosporioides</i> from <i>Stylosanthes</i> spp. in Africa and Australia using RAPD and ribosomal DNA markers

Munaut, Françoise; Hamaide, Nancy; Stappen, J. Vander; Maraite, Henri

doi:10.1046/j.1365-3059.1998.00287.x

Cited by 22 publications

(29 citation statements)

References 23 publications

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“…Another approach for genetic diversity analyses has been the use of the technique of random amplified polymorphic DNA (RAPD), resulting in amplification of DNA from the entire genome based on arbitrary 10-base oligonucleotide primers (Welsh and McClelland 1990;Williams et al 1990). RAPD analysis has become increasingly popular to explore genetic variability in basidiomycetes (Khush et al 1992;Chiu et al 1996) and other fungi (Francis et al 1994;Munau et al 1998;Morris et al 2000). The RAPD technique permits amplification of all genomic DNA, so it may provide a better representation of the genome structure than analysis targeting a single locus.…”

Section: Introductionmentioning

confidence: 99%

Genetic relationships in the natural population of Pholiota nameko from Japan based on DNA polymorphisms

Obatake¹,

Matsumoto

Mimura

et al. 2002

Mycoscience

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This study characterized the genetic relationships in the natural population of Pholiota nameko (Strophariaceae) from Japan based on the RFLPs of two regions of nuclear rDNA (ITS and IGS) and mitochondrial DNA and the RAPD profile of nuclear DNA. No intraspecific polymorphism in rDNA was found among 36 isolates of P. nameko used. By contrast, digests of mtDNAs by endonucleases HindIII and BglII produced RFLP patterns that distinguished all isolates except for 2, and clustered 36 wild isolates phenetically into three major similarity groups. However, these groups as obtained by analysis of mtDNA RFLPs did not reflect the geographic origin of the isolates. In RAPD analysis of nuclear DNA using three kinds of primers, every isolate showed its own distinct RAPD profile, but all isolates were clustered only into a large similarity group by phylogenetic analysis based on the RAPD profile. From these results, it is suggested that wild isolates of P. nameko distributed in Japan form a continuous genetic population that has conserved the genetic diversity.

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Section: Introductionmentioning

confidence: 99%

Genetic relationships in the natural population of Pholiota nameko from Japan based on DNA polymorphisms

Obatake¹,

Matsumoto

Mimura

et al. 2002

Mycoscience

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“…For C. gloeosporioides, the number of nuclei in conidia was more variable than previously indicated and may be dependent on cultural conditions. However, Munaut et al [53] maintained that the probability of isolating a heterokaryotic conidium was low. Different ITS types were also reported for different nuclei of Sclerotium species [54] and Scutellospora isolates [55].…”

Section: Discussionmentioning

confidence: 99%

Genetic Differentiation of Colletotrichum gloeosporioides and C. truncatum Associated with Anthracnose Disease of Papaya (Carica papaya L.) and Bell Pepper (Capsium annuum L.) Based on ITS PCR-RFLP Fingerprinting

Maharaj

Rampersad

2011

Mol Biotechnol

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Members of the genus Colletotrichum include some of the most economically important fungal pathogens in the world. Accurate diagnosis is critical to devising disease management strategies. Two species, Colletotrichum gloeosporioides and C. truncatum, are responsible for anthracnose disease in papaya (Carica papaya L.) and bell pepper (Capsicum annuum L.) in Trinidad. The ITS1-5.8S-ITS2 region of 48 Colletotrichum isolates was sequenced, and the ITS PCR products were analyzed by PCR-RFLP analysis. Restriction site polymorphisms generated from 11 restriction enzymes enabled the identification of specific enzymes that were successful in distinguishing between C. gloeosporioides and C. truncatum isolates. Species-specific restriction fragment length polymorphisms generated by the enzymes AluI, HaeIII, PvuII, RsaI, and Sau3A were used to consistently resolve C. gloeosporioides and C. truncatum isolates from papaya. AluI, ApaI, PvuII, RsaI, and SmaI reliably separated isolates of C. gloeosporioides and C. truncatum from bell pepper. PvuII, RsaI, and Sau3A were also capable of distinguishing among the C. gloeosporioides isolates from papaya based on the different restriction patterns that were obtained as a result of intra-specific variation in restriction enzyme recognition sites in the ITS1-5.8S-ITS2 rDNA region. Of all the isolates tested, C. gloeosporioides from papaya also had the highest number of PCR-RFLP haplotypes. Cluster analysis of sequence and PCR-RFLP data demonstrated that all C. gloeosporioides and C. truncatum isolates clustered separately into species-specific clades regardless of host species. Phylograms also revealed consistent topologies which suggested that the genetic distances for PCR-RFLP-generated data were comparable to that of ITS sequence data. ITS PCR-RFLP fingerprinting is a rapid and reliable method to identify and differentiate between Colletotrichum species.

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“…Similarly, biotypes A and B and putative biotype C from Africa have been described [14]. The diversity among strains pathogenic on Stylosanthes and their relationship with other strains were analyzed at the molecular level using various markers, such as dsRNA [15], RFLP [16], [17], RAPD [14], [18]–[20], and ITS [21]. The diversity among the pathogen population from Brazil, Colombia, China, and India is extensive [19].…”

Section: Introductionmentioning

confidence: 99%

Cloning of Insertion Site Flanking Sequence and Construction of Transfer DNA Insert Mutant Library in Stylosanthes Colletotrichum

Chen

et al. 2014

PLoS ONE

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Stylosanthes sp. is the most important forage legume in tropical areas worldwide. Stylosanthes anthracnose, which is mainly caused by Colletotrichum gloeosporioides, is a globally severe disease in stylo production. Little progress has been made in anthracnose molecular pathogenesis research. In this study, Agrobacterium tumefaciens-mediated transformation was used to transform Stylosanthes colletotrichum strain CH008. The major factors of the genetic transformation system of S. colletotrichum were optimized as follows: A. tumefaciens’ AGL-1 concentration (OD600), 0.8; concentration of Colletotrichum conidium, 1×106 conidia/mL; acetosyringone concentration, 100 mmol/L; induction time, 6 h; co-culture temperature, 25°C; and co-culture time, 3 d. Thus, the transformation efficiency was increased to 300–400 transformants per 106 conidia. Based on the optimized system, a mutant library containing 4616 mutants was constructed, from which some mutants were randomly selected for analysis. Results show that the mutants were single copies that could be stably inherited. The growth rate, spore amount, spore germination rate, and appressorium formation rate in some mutants were significantly different from those in the wild-type strain. We then selected the most appropriate method for the preliminary screening and re-screening of each mutant’s pathogenic defects. We selected 1230 transformants, and obtained 23 strains with pathogenic defects, namely, 18 strains with reduced pathogenicity and five strains with lost pathogenicity. Thermal asymmetric interlaced PCR was used to identify the transfer DNA (T-DNA) integration site in the mutant that was coded 2430, and a sequence of 476 bp was obtained. The flanking sequence of T-DNA was compared with the Colletotrichum genome by BLAST, and a sequence of 401 bp was found in Contig464 of the Colletotrichum genome. By predicting the function of the flanking sequence, we discovered that T-DNA insertion in the promoter region of the putative gene had 79% homology with the aspartate aminotransferase gene in Magnaporthe oryzae (XP_003719674.1).

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Genetic relationships among isolates of Colletotrichum gloeosporioides from Stylosanthes spp. in Africa and Australia using RAPD and ribosomal DNA markers

Cited by 22 publications

References 23 publications

Genetic relationships in the natural population of Pholiota nameko from Japan based on DNA polymorphisms

Genetic relationships in the natural population of Pholiota nameko from Japan based on DNA polymorphisms

Genetic Differentiation of Colletotrichum gloeosporioides and C. truncatum Associated with Anthracnose Disease of Papaya (Carica papaya L.) and Bell Pepper (Capsium annuum L.) Based on ITS PCR-RFLP Fingerprinting

Cloning of Insertion Site Flanking Sequence and Construction of Transfer DNA Insert Mutant Library in Stylosanthes Colletotrichum

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