Genetic relationship of Tricholoma matsutake and T. nauseosum from the Northern Hemisphere based on analyses of ribosomal DNA spacer regions

Abstract

The genetic relationship among Tricholoma matsutake and T. nauseosum strains collected from various parts of the Northern Hemisphere was investigated using sequence analysis of the rDNA ITS region and PCR-RFLP analysis of the rDNA IGS-1 region. ITS sequence similarity between T. matsutake and T. nauseosum ranged between 98.1% and 100%. The strains of T. matsutake from coniferous forests and those from broad-leaved forests showed more than 99.8% similarity in their ITS sequences. Three distinct RFLP types were detected when IGS-1 regions were digested with Cfr13I. RFLP patterns showed no variability among the strains of T. nauseosum and those of T. matsutake from broad-leaved forests. This pattern corresponded to the dominant RFLP type in the Japanese population of T. matsutake. Thus, strains belonging to this RFLP type are widely distributed throughout East Asia and Europe and associated with many tree species of Pinaceae and Fagaceae. The result suggests that T. matsutake in coniferous and broad-leaved forests and T. nauseosum should be treated as the same species genetically.

A new record of Tricholoma caligatum (Tricholomataceae) from North Africa with a discussion of related species

“…All specimen of T. nauseosum found under this tree in Morocco actually belong to T. anatolicum as revealed with molecular studies (Ota et al 2012). Then, we predicate that specimens collected in North Africa were misidentified and are T. anatolicum and not T. nauseosum because T. nauseosum is synonym of T. matsutake (Kytövuori 1988) ), which is confirmed by molecular study by Bergius & Danell (2000) and Matsushita et al (2005). The presence of T. matsutake in North Africa as not yet been demonstrated.…”

“…Later, Iwase, (1994) distinguished three types of T. caligatum: T. caligatum1 distributed in North Africa and Southern Europe, T. caligatum2 found in western North America and associated with conifers, and T. caligatum3 found in eastern North America and associated with oak trees. Matsushita et al (2005), based on a molecular analysis, suggested that T. nauseosum and T. matsutake were the same species (current name in Index fungorum: T. matsutake). Chapela & Garbelotto (2004), using ITS polymorphism, considered T. caligatum as a polyphyletic group.…”

Traceability of Asian Matsutake , Specialty Mushrooms Produced by the Ectomycorrhizal Basidiomycete Tricholoma matsutake , on the Basis of Retroelement-Based DNA Markers

“…In T. matsutake, however, the sequences of both intergenic spacer 1 (IGS1) and the interspacer regions (ITS1 and ITS2) are highly conserved among isolates from all over the world (10,15). We previously demonstrated that T. matsutake has megB1, an approximately 500-bp DNA segment that is the major component of IGS1 from many mushroom species including species of Pleurotus, in a genomic region out of rDNA (3) (AB304914 and AB304915).…”

First record of Tricholoma fulvocastaneum from Thailand

“…ITS sequences of basidiome (GenBank accession no. DQ067895, 830 bp) collected in Thailand share 99% homology with those of T. fulvocastaneum available on GenBank; AB036901 (direct submission by H. Murata) and AF204808 (Kikuchi et al 2000), and it is distant from other Asian Tricholoma spp., including T. matsutake and T. bakamatsutake, as shown by Matsushita et al (2005).…”