Genetic relationship between the adult plant resistance gene <i>Lr12</i> and the complementary gene <i>Lr31</i> for seedling resistance to leaf rust in common wheat

Singh, Davinder; Park, Robert; McIntosh, James R.

doi:10.1046/j.1365-3059.1999.00391.x

Cited by 29 publications

(19 citation statements)

References 14 publications

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“…Five cultivars (Sakha94, Gemmeiza9, Gemmeiza10, Sids12 and Misr2) each probably containing four genes however, Gemmeiza11 and Sids13 were the least carrier genes cultivars they probably carries just two and three genes, respectively. These results were in accordance to those previously reported [24][25][26][27][28][29][30][31].…”

Section: Discussionsupporting

confidence: 94%

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Identification of Leaf Rust Resistance Genes in Egyptian Wheat Cultivars by Multipathotypes and Molecular Markers

Abou-Elseoud¹

2014

JPS

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A total of twenty leaf rust resistance genes (Lr genes) were postulated in nine Egyptian wheat cultivars based on infection types (ITs) expressed on the tested cultivars by 72 Puccinia triticina pathotypes compared with the ITs expressed on the monogenic lines. The most carrier genes cultivars were Giza168 and Misr1 each may contain five genes i.e. Lr2c,10,18,24, 41 and Lr3,10,19, 22b,24, respectively. Five cultivars, Sakha94, Gemmeiza9, Gemmeiza10, Sids12 and Misr2 each probably contain four genes i.e. Lr9,19,29, 37; Lr18,21,24, 41; Lr3,9,19,29; Lr9,19,26, 29 and Lr3,10,19,26, respectively. Gemmeiza11 was the least cultivar carrying genes; it probably carries just two genes i.e. Lr24 and Lr41. The most postulated genes were Lr19 and Lr24, each postulated within five cultivars followed by Lr41 within four cultivars. Five Lr genes, Lr3, Lr9, Lr10, Lr26 and Lr29 each within three cultivars. The lowest postulated genes were Lr2c, Lr21, Lr22b and Lr37 each of them was postulated within only one cultivar. Five Lr genes, Lr9, Lr10, Lr19, Lr24 and Lr26 were identified by PCR-based molecular marker. The Lr9 gene was identified in cultivar Sids12 while, Lr10 was identified in cultivar Misr1. The Lr19 was present in two cultivars, Misr1 and Misr2. The Lr24 and Lr26 were absent in all the screened Egyptian cultivars. The obtained results for Lr9, Lr10, Lr19, Lr24 and Lr26 marker were in agreement with and confirm their identification by gene postulation. Markers for Lr9, Lr10 and Lr19 may be useful in marker-assisted breeding. Our findings showed the usefulness of the molecular marker in identifying leaf rust resistance genes in wheat cultivars, especially when used in conjunction with multipathotypes test at the pre-breeding stage. This approach may help understanding the wheat -P. triticina interaction and provide information to build an effective management program for leaf rust disease.

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Section: Discussionsupporting

confidence: 94%

“…Five Lr genes (Lr3, Lr9, Lr10, Lr26 and Lr29) each giving 33.33% frequencies within three cultivars. The method was previously reported as a quick method to serve breeding for rust resistance [11,12,[24][25][26][27][28][29][30].…”

Section: Discussionmentioning

confidence: 99%

Identification of Leaf Rust Resistance Genes in Egyptian Wheat Cultivars by Multipathotypes and Molecular Markers

Abou-Elseoud¹

2014

JPS

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“…Wellings (1986) crossed susceptible with resistant selections of cultivars heterogeneous for the YrA resistance, and seedling tests at both the F 2 and F 3 generations confirmed segregation for a single gene suggesting that susceptible selections carried one of the complementary genes. was previously reported, where Lr27 interacted with Lr31 to produce a unique seedling specificity (Singh and McIntosh 1984;Singh et al 1999). was previously reported, where Lr27 interacted with Lr31 to produce a unique seedling specificity (Singh and McIntosh 1984;Singh et al 1999).…”

Section: Introductionmentioning

confidence: 78%

“…Complementary genes for rust resistance have been reported on several occasions in both greenhouse and field studies. In Gatcher wheat Lr27 is ineffective on its own, but at the seedling stage, interacts with adult plant resistance gene Lr12 (same as Lr31) to provide resistance (Singh et al 1999). A common feature of both studies was that crosses of susceptible lines reconstituted the resistance specificity and segregated in expected F 2 and F 3 ratios.…”

Section: Discussionmentioning

confidence: 99%

Complementary resistance genes in wheat selection ‘Avocet R’ confer resistance to stripe rust

et al. 2015

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Complementary genes for resistance to wheat stripe rust in an Avocet selection mapped to chromosome arms 3DL and 5BL. Susceptible Avocet selections lacked the 5BL gene due to a chromosomal deletion. This study reports the inheritance and genetic mapping of the YrA (temporary name of convenience to describe the specificity) seedling resistance to wheat stripe rust (caused by Puccinia striiformis f. sp. tritici; Pst) in a resistant selection of the Australian cv. Avocet [Avocet R (AvR)-AUS 90660]. Genetic analysis was performed on F2 populations and F3 generation families from crosses between wheats that carried and lacked the YrA resistance. Greenhouse seedling tests with two avirulent Pst pathotypes (104 E137 A- and 108 E141 A-) confirmed that the YrA resistance was inherited as two complementary dominant genes. Ninety-two doubled haploid (DH) lines from a cross between the Australian cv. Teal (Pst susceptible) and AvR were used for DArT-Seq genotypic analysis to map the seedling resistance. Marker-trait association analysis using 9035 DArT-Seq loci mapped the genes to the long arms of chromosomes 3D (3DL) and 5B (5BL), respectively. F2 populations from crosses between susceptible DH lines that carried either the 3DL or 5BL marker genotypes confirmed the complementary gene model. Fluorescence in situ hybridization (FISH) analysis determined that Teal carries a reciprocal T5B-7B translocation. FISH analysis also identified a 5BL chromosomal deletion in Avocet S relative to AvR that further validated the complementary gene model and possibly explained the heterogeneity of closely related wheats carrying the YrA resistance. The individual loci of the complementary YrA resistance were designated Yr73 (3DL) and Yr74 (5BL). Candidate single gene reference stocks will be permanently accessioned following cytological analysis to avoid the T5B-7B translocation.

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“…Genes Lr13 (Samborski and Dyck, 1982;Kolmer, 1992;Kloppers and Pretorius, 1997) and Lr34 (German and Kolmer, 1992;Kloppers and Pretorius, 1997) in combination with each other and with a number of other genes generate stronger resistance than that conditioned by the individual genes. The Lr27 locus on 3BS is closely linked with Sr2, which was introgressed from Yaroslav Emmer (McIntosh et al, 1995), whereas Lr31 occurs on 4BS of Chinese Spring and may be identical to the APR gene Lr12 (Singh et al, 1999). The Lr27 locus on 3BS is closely linked with Sr2, which was introgressed from Yaroslav Emmer (McIntosh et al, 1995), whereas Lr31 occurs on 4BS of Chinese Spring and may be identical to the APR gene Lr12 (Singh et al, 1999).…”

Section: Researchmentioning

confidence: 99%

Transfer of Leaf Rust and Stripe Rust Resistance Genes Lr62 and Yr42 from Aegilops neglecta Req. ex Bertol. to Common Wheat

Marais

McCallum

et al. 2009

Crop Science

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Continued genetic control of the cereal rusts depends on the availability of effective resistance genes. The wild relatives of wheat (Triticum aestivum L.) constitute a source of such genes. Wheat leaf rust (Puccinia triticina Eriks.) resistant Aegilops neglecta accession 155 was crossed with ‘Chinese Spring’. Resistant progeny were initially backcrossed to Chinese Spring and later to Chinese Spring‐Short (a short‐strawed near‐isogenic line) to develop an addition line. Advanced backcross progeny segregated for a resistance gene (designated Lr62) located on an addition chromosome, as well as a resistant phenotype apparently contributed by dominant complementary genes of the wheat genomes. On its own, Lr62 produced infection type (IT); however, in the presence of the two complementary genes its expression was modified to an intermediate response. The addition chromosome appeared to have homeology with group 3 chromosomes of wheat. While attempting to transfer the resistance through allosyndetic pairing induction to a group 3 wheat chromosome, a spontaneous translocation occurred. Aneuploid and microsatellite analyses showed that the translocation involved wheat chromosome 6A, suggesting that the addition chromosome may also have partial homeology to group 6. Microsatellite and meiotic pairing data suggested the presence of a large segment of foreign chromatin replacing the entire 6AS arm and a proximal part of 6AL. Lr62 was effective against a wide range of South African and western Canadian Puccinia triticina pathotypes. In addition to Lr62, the translocation line, 03M119‐71A, carried a seedling stripe rust resistance gene (designated Yr42) effective against South African pathotypes of P. striiformis The resistance genes can be of significant commercial value, however, it may be necessary to further tailor this fairly big translocation through allosyndetic pairing induction.

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Genetic relationship between the adult plant resistance gene Lr12 and the complementary gene Lr31 for seedling resistance to leaf rust in common wheat

Cited by 29 publications

References 14 publications

Identification of Leaf Rust Resistance Genes in Egyptian Wheat Cultivars by Multipathotypes and Molecular Markers

Identification of Leaf Rust Resistance Genes in Egyptian Wheat Cultivars by Multipathotypes and Molecular Markers

Complementary resistance genes in wheat selection ‘Avocet R’ confer resistance to stripe rust

Transfer of Leaf Rust and Stripe Rust Resistance Genes Lr62 and Yr42 from Aegilops neglecta Req. ex Bertol. to Common Wheat

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