Genetic Recombination Between Escherichia Coli and Salmonella Typhimurium

Baron, L. S.; Carey, Warren F.; Spilman, W. M.

doi:10.1073/pnas.45.7.976

Cited by 50 publications

(23 citation statements)

References 17 publications

(2 reference statements)

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“…These results support the premise that genetic recombination may have played a role in the evolution of the family Enterobacteriaceae. The data indicate also that hybrids recovered from in vivo matings are similar to those reported from in vitro crosses (3,4).…”

Section: Experiments By Luria and Burroussupporting

confidence: 77%

“…As was shown earlier, the hybrids appeared shortly after the S. typhimurium was introduced and increased rapidly indicating hybrid multiplication and making the determination of frequencies difficult. DISCUSSION The extensive studies of in dtro genetic recombination between E. coli and Shigella by Lurla and Burrous (1) and between E. coli and Salmonella by Baron et al (2)(3)(4) have demonstrated conclusively the interfertility existing between these organisms. Although the occurrence of organisms bearing characteristics of two established species may be the result of similar genetic mechanisms, the role of this phenomenon in nature has not been established.…”

Section: Resultsmentioning

confidence: 95%

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Experimental Genetic Recombination in Vivo Between Escherichia Coli and Salmonella Typhimurium

Schneider

Formal

Baron

1961

The Journal of Experimental Medicine

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Antibiotic-pretreated mice were fed orally an Hfr culture of streptomycin-resistant E. coli and 1 day later, a streptomycin-resistant F- S. typhimurium culture. Hybrids were recovered in relatively small numbers from the feces of these mice within 24 hours demonstrating that genetic recombination can occur within the intestinal tract of a mammalian host under experimental conditions. These hybrids multiplied rapidly and persisted throughout the course of the experiment. In addition, hybrids were recovered which had not been observed in single matings performed in vitro.

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Section: Experiments By Luria and Burroussupporting

confidence: 77%

Section: Resultsmentioning

confidence: 95%

Experimental Genetic Recombination in Vivo Between Escherichia Coli and Salmonella Typhimurium

Schneider

Formal

Baron

1961

The Journal of Experimental Medicine

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“…S. typhosa in our experience appears somewhat unique among the Salmonellae examined, in that it behaves as a homogeneously fertile population with regard to its recipient ability in matings with E. coil K-12 donors (14). This ability in S. typhimurium, for example, is found only in rare or mutant cells of that population (2,9,17) and is believed by some workers (7,18) to be explainable in terms of host controlled restriction and modification of DNA (1,5,8). At the present time we cannot properly compare the behavior of S. typhosa as a recipient of E. coil DNA with that of fertile S. typhimurlum strains, primarily because the formation of partial diploid hybrids in those S. typhimurium strains has not been sufficiently investigated.…”

Section: Resultsmentioning

confidence: 70%

Inefficiency of Genetic Recombination in Hybrids Between Escherichia coli and Salmonella typhosa

1971

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An Escherichia coli Hfr strain in which three negative chromosomal alleles (leu-, arg-, and mtl-) were closely linked to three positive alleles (ara+, rha+, and xyl+, respectively) was employed in matings with a Salmonella typhosa recipient. The detected expression of the negative E. coli alleles in S. typhosa hybrids selected for receipt of an associated positive E. coli marker was used to determine the occurrence of haploid S. typhosa recombinants, as distinguished from stable partial diploid hybrids. At the same time, the inheritance patterns and segregation behavior of the positive alleles provided indicators of the occurrence of partial diploid hybrids. Examination of both positive and negative markers inherited by ara+, rha+ and xyl+ selected S. typhosa hybrid classes indicated that relatively short E. coli chromosomal segments (generally about 4 min or less in length) were involved in recombination (haploidy), whereas rather extensive E. coli genetic segments were conserved in the diploid state. S. typhosa hybrids selected for receipt of the ara+ marker showed a 52% incidence of leuhaploidy, which is probably close to being an accurate measure of recombination at the site of the ara+ allele. S. typhosa hybrids selected for receipt of the rha+ or xyl+ markers showed only a 20% incidence of argor mtlhaploidy, respectively, but both of these hybrid classes exhibited a higher incidence of conservation of extensive E. coil diploid segments than did the ara+ selected class. Remating of haploid S. typhosa hybrids with recombinant xyl+ mtlor rha+ arg-regions resulted in higher frequencies of hybrid recovery than were observed in the initial matings. However, there was a higher incidence of partial diploidy and a lower incidence of haploidy among the hybrids obtained from these rematings.

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“…Campbell 17proposed to include among episomes other hereditary cytoplasmic factors, whose integrated state has not yet been proved. For example, "lac-episome" (13,14,15,22), "sporulation factor" in Bacillus species (47), mycelial factor in Aspergillus (100), and "controlling elements" in maize (69) would be regarded as episomes, in addition to temperate phages, F agents, colicinogenic factors, and resistance transfer factor. According to Campbell, these episomes could be divided into two classes, namely, temperate phages (or, more strictly, the genetic material thereof), and transfer factors.…”

Section: Concept Of Episomesmentioning

confidence: 99%