Experimental Genetic Recombination in Vivo Between Escherichia Coli and Salmonella Typhimurium

Schneider, Herman; Formal, Samuel B.; Baron, L. S.

doi:10.1084/jem.114.1.141

Cited by 17 publications

(9 citation statements)

References 8 publications

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“…Since the diploid forms are similar to E. coli by virtue of acquired biochemical and antigenic factors, the difficulties presented to the diagnostic bacteriologist are apparent. It is likely also that matings of the sort described here may occur in nature; this view is supported by recent results obtained in this laboratory which have demonstrated in ~ivo recombination between E. coli and S. typhimurium in mice infected with S. typhimurium (21). A lactose-positive (and indol-positive) strain would almost certainly be overlooked or disregarded on isolation as being without significance as an enteric pathogen.…”

Section: Discussionsupporting

confidence: 71%

Diploid Heterozygous Hybrids From Matings Between Escherichia Coli and Salmonella Typhosa

Baron

Spilman

Carey

1960

The Journal of Experimental Medicine

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Sexuality in bacteria (1), now considered to be a unilateral conjugation (2, 3), has been studied in Escherichia coli b y m a n y workers. Luria and Burrous (4) have extended this form of genetic recombination to bacteria classified in different genera with their description of mating between E. coli and Shigella species. Recently, successful crosses between E. coli and Salmonella typhimurium were reported b y Baron eta/. (5). These studies dealt with the isolation of a streptomycin-resistant m u t a n t of S. typhimurium which acted as a high frequency recipient strain in matings with E. coli. Further studies b y Baron et al. (6) Materials and MethodsCultures.--The strains of S. typhosa employed were obtained from the culture collection at the Waiter Reed Army Institute of Research. A high frequency of recombination (Hfr) derivative of E. ¢otl K-12, strain W-1895, was obtained from Dr. P. D. Skaar, and a D-arabinose utilizing (v-Ara) mutant of this organism was isolated and employed in these studies.Media.--Meat extract agar, minimal media, EMB agar, and other culture media were used as described previously (5).Antisera.--Rabbit antisera were prepared against E. coU, S. typhosa, and selected hybrids by a series of intravenous injections of 109 viable or heat-killed (100°C. for 2 hours) cells. Absorbed sera were prepared as described by Edwards and Ewing (7).Phages.--The Vi typing phages were obtained from Dr. Cora Gunther; the procedures used for propagating these phages have been described by Baron et aL (8). The T series of bacteriophages were received from Dr. G. Bertani.Recombination Procedures.--For the detection of strains fertile with E. coli K-12, Hfr strain W-1895, the various cultures of S. typhosa which were to be tested as recipients (females) were spread with the E. coli culture as the donor (male) strain at a ratio of twenty recipient cells to one donor cell on an appropriate selective medium. The E. coli culture was grown in

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Section: Discussionsupporting

confidence: 71%

Diploid Heterozygous Hybrids From Matings Between Escherichia Coli and Salmonella Typhosa

Baron

Spilman

Carey

1960

The Journal of Experimental Medicine

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“…The limited number of converted recipient cells obtained in these experiments requires comment, since, in the in vivo experiments of Schneider and co-workers (11), the number of hybrid cells was frequently larger than the number of donor or recipient cells. In our experiments, converted recipients were always outnumbered by donor cells and never exceeded the number of recipient cells ( Fig.…”

Section: Discussionmentioning

confidence: 94%

Transfer of Infectious Drug Resistance in Microbially Defined Mice

1969

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Germ-free mice were intentionally associated with drug-resistant donor strains of Escherichia coli known to carry R factors and with drug-sensitive recipient strains. In vivo transfer of R factors was observed in all experiments, involving five different donor-recipient combinations. The number of converted recipients varied, depending upon the donor-recipient combination, but in all cases it was restricted by limiting numbers of either recipient or donor strains in the digestive tract of the microbially defined mice. Converted recipients were detected in fecal material as early as 5.5 hr after mice were associated with donor and recipient bacteria. Donors, recipients, and converted recipients were detectable in the stomach, small intestine, cecum, and large intestine of the microbially defined mice and their suckling young.

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“…In vivo transfer of plasmids has been reported in the intestinal tract of gnotobiotic or antibiotic treated mice, pigs and humans (Schneider, Formal & Baron, 1961 ;Jones & Curtis, 1969 ;Mitsuhashi, I 971). Weinberg & Statzky (1972) have evidence for plasmid transfer in sterile soil.…”

Section: Introductionmentioning

confidence: 99%

Transfer of Antibiotic Resistance Plasmid RP1 into Pseudomonas glycinea and Pseudomonas phaseolicola in vitro and in planta

Lacy¹,

Leary²

1975

Journal of General Microbiology

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The wide host-range antibiotic resistance plasmid RPI was transferred from Pseudomonas aeruginosa via Escherichia coli into Pseudomonas glycinea. The plasmid was then acquired by Pseudomonas phaseolicola both in vitro and in planta in Phaseolus limensis leaves and pods. This was the first step in the design of a model system to determine the possible epidemiological significance of antibiotic resistance plasmids in the control of plant disease.

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Experimental Genetic Recombination in Vivo Between Escherichia Coli and Salmonella Typhimurium

Cited by 17 publications

References 8 publications

Diploid Heterozygous Hybrids From Matings Between Escherichia Coli and Salmonella Typhosa

Diploid Heterozygous Hybrids From Matings Between Escherichia Coli and Salmonella Typhosa

Transfer of Infectious Drug Resistance in Microbially Defined Mice

Transfer of Antibiotic Resistance Plasmid RP1 into Pseudomonas glycinea and Pseudomonas phaseolicola in vitro and in planta

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