Genetic Polymorphisms of Insulin-Like Growth Factor 1 Are Associated with Osteosarcoma Risk and Prognosis

Mao, Jianshui; Zhuang, Genying; Chen, Zhikang

doi:10.12659/msm.908004

Cited by 15 publications

(9 citation statements)

References 21 publications

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“…For example, at 48 hours of exposure to 50 μg/ml of GO, the total percentage of apoptotic cells for the U2OS IGF-1 knocked out, and U2OS IGFBP3 knocked out were 49.1% and 45.9%, respectively compared to 26.28% for U2OS. Knocking out IGF-1 was slightly more effective than knocking out the IGFBP3 gene [51,52]. The results from flow cytometry and morphological changes support the hypothesis that GO has a toxic effect on human cancer cells but not on healthy cells.…”

Section: Igf Plays a Vital Role In The Growth And Development Of Tisssupporting

confidence: 66%

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Graphene Oxide Nanoparticles Induce Apoptosis in wild-type and CRISPR/Cas9-IGF/IGFBP3 knocked-out Osteosarcoma Cells

et al. 2020

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Osteosarcoma affects both adolescents and adults, and some improvement in the survival rate for affected patients has been reached in the last decade. Still, non-specificity and systemic toxicity may limit traditional therapeutic approaches to some extent. The insulin growth factor 1 (IGF1) and its binding protein (IGFBP3) have been implicated in the tumorigenesis. Nanoparticles, such as graphene oxide (GO), can provide an effective treatment for cancer as they can specifically target cancer cells while reducing undesired side effects. This study aimed to evaluate the toxicity of GO on osteosarcoma in vitro using tumor cell lines with and without knocking out the IGF and IGFBP3 genes. Human osteosarcoma cell lines, U2OS and SAOS2, and the normal osteoblast cell line hFOB1.19 were used. The IGF1 and IGFBP3 genes were eliminated using CRISPR/Cas9. Tumor cells were cultured and treated with GO. Apoptosis and reactive oxygen species (ROS) were analyzed by Annexin V-FITC and ROS assays. The nuclear factor erythroid 2-related factor 2 (NRF2), which is a crucial regulator of cellular resistance to oxidants, was investigated by Western blotting. We found a significantly higher rate of apoptosis in the OS than hFOB1.19, especially in U2OS cells in which IGF1 and IGFBP3 were knocked out. ROS increase due to GO exposure was remarkably time and concentration-dependent. Based on the rate of apoptosis, ROS, Nrf-2 decrease, and cytomorphological changes, GO has a significant cytotoxic effect against OS. Targeting the IGF1 and IGFBP3 signaling pathway may strengthen GO-related cytotoxicity with the potential to increase the survival of patients affected by this tumor.

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Section: Igf Plays a Vital Role In The Growth And Development Of Tisssupporting

confidence: 66%

“…There is evidence that IGF-1 related pathways are involved in osteosarcoma progression. There is downstream of signaling pathways such as the MAPK, PI3K-Akt, and NF-kB pathways [51,52].…”

Section: Igf Plays a Vital Role In The Growth And Development Of Tissmentioning

confidence: 99%

Graphene Oxide Nanoparticles Induce Apoptosis in wild-type and CRISPR/Cas9-IGF/IGFBP3 knocked-out Osteosarcoma Cells

et al. 2020

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“…However, the relatively small sample size of the three studies in this meta-analysis may reduce the statistical strength of the test correlation, leading to debates on the results and impact on the conclusions (11,19,21). Compared with different races and populations, our meta-analysis showed that there was no significant correlation between IGF1rs5742612 polymorphism and malignant tumor susceptibility in Iran (16,19) or South Korea (20) population, which is inconsistent with the Chinese population (17,18,21). Due to the diversity and potential differences in malignant tumor susceptibility in the population, the results of the current meta-analysis may not be generalized, so further research is needed on other races and populations to provide additional evidence.…”

Section: Discussionmentioning

confidence: 99%

Association between insulin-like growth factor 1 gene rs5742612 polymorphism and malignant tumor susceptibility: a meta-analysis

Zhou¹,

Zhang²,

Zheng³

et al. 2021

Transl Cancer Res TCR

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Background: Malignant tumor is a serious threat to human health and life, which is a difficult problem in the world. Insulin-like growth factor 1 (IGF1) is an important mitotic factor in vivo. It usually acts in the way of autocrine and paracrine to control the proliferation, differentiation, and apoptosis of various cells, IGF1 has a strong mitotic and anti-apoptosis activity in malignant cells. Single nucleotide polymorphism (SNP) is an important part of individual genetic variation. A large number of studies have shown that IGF1 SNP associated with the risk of a malignant tumor may be an important biomarker for the diagnosis of malignant tumors. Therefore, the article will investigate the association between rs5742612 polymorphism of IGF1 gene and malignant tumor susceptibility. Methods:We searched for studies in five databases (PubMed, Embase, Web of Science, CNKI and Wanfang) regarding the association between IGF1 gene rs5742612 and malignant tumor susceptibility. Odds ratios (ORs) and the related 95% confidence intervals (CIs) were employed to assess the strength of the associations.Results: Ultimately this study identified seven articles that met the inclusion criteria, involving 2,581 cases and 2,445 controls. There was no significant correlation between IGF1 gene rs5742612 polymorphism and malignant tumor susceptibility [thymidine (T) vs. cytimidine (C),

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“…Several polymorphisms, including rs6214, rs6220, rs5742714, rs1549593, 2,373,722, 10,735,380, 12,821,878, rs2195239, rs2162679, rs35767, rs5742612, and rs7965399, have been reported to be related to disease occurrence [15, 18, 20–23, 32–34]. Some of these important polymorphisms, such as rs6214, rs6220, and rs5742714,are located in the 3’UTR region of IGF1 , while others, such as rs1549593, 2,373,722, 10,735,380, 12,821,878, rs2195239, and rs2162679, are located in the intron of IGF1 .…”

Section: Discussionmentioning

confidence: 99%

Association between the insulin-like growth factor 1 gene rs2195239 and rs2162679 polymorphisms and cancer risk: a meta-analysis

Chen

Zhao

et al. 2019

BMC Med Genet

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BackgroundMany epidemiological studies have suggested that insulin-like growth factor1 (IGF1) gene single-nucleotide polymorphisms (SNPs) may be associated with cancer risk. Among several commonly studied polymorphisms in IGF1 gene, rs2195239 and rs2162679 attracted many attentions. So we perform a meta-analysis to determine potential associations between IGF1 rs2195239 and rs2162679 polymorphisms and cancer risk.MethodsWe retrieved relevant articles from the PubMed, Embase, and Web of Science databases up to April 30, 2018. Ultimately, thirteen studies were included in the present meta-analysis, which involved 12,515 cases and 19,651 controls. The odd ratios (ORs) and their 95% confidence intervals (CIs) were pooled to estimate the strength of the associations.Resultsrs2195239 reduces the overall cancer risk in homozygote model, as well as reducing cancer risk in Asian populations in allele, homozygote, and recessive models. No significant relationship was found between rs2195239 and breast or pancreatic cancer risk. rs2162679 reduces the overall cancer risk in allele, homozygote, dominant, and recessive models, as well as reducing cancer risk in Asian populations in allele, homozygote, and recessive models.ConclusionsIGF1 rs2195239 and rs2162679 were associated with overall cancer risk based on present studies.Electronic supplementary materialThe online version of this article (10.1186/s12881-019-0749-3) contains supplementary material, which is available to authorized users.

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Genetic Polymorphisms of Insulin-Like Growth Factor 1 Are Associated with Osteosarcoma Risk and Prognosis

Cited by 15 publications

References 21 publications

Graphene Oxide Nanoparticles Induce Apoptosis in wild-type and CRISPR/Cas9-IGF/IGFBP3 knocked-out Osteosarcoma Cells

Graphene Oxide Nanoparticles Induce Apoptosis in wild-type and CRISPR/Cas9-IGF/IGFBP3 knocked-out Osteosarcoma Cells

Association between insulin-like growth factor 1 gene rs5742612 polymorphism and malignant tumor susceptibility: a meta-analysis

Association between the insulin-like growth factor 1 gene rs2195239 and rs2162679 polymorphisms and cancer risk: a meta-analysis

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