Genetic polymorphism of serum postalbumin (Po) and pretransferrin (Prt) in the laboratory rabbit

Abstract

. 1984. Genetic polymorphism of serum postalbumin (Po) and pretransferrin (Prt) in the laboratory rabbit. -Hereditas 100: 11-15. Lund, Sweden. ISSN 0018- Received March 14,1983A new genetic polymorphism of a rabbit serum protein, designated postalbumin (Po) was detected by two-dimensional agarose gel (pH 8.6)-polyacrylamide gel (pH 9.0) electrophoresis. Family data indicated that the three different Po phenotypes observed were controlled by two codominant, autosomal alleles PoF and Pos). Considerable polymorphism of Po was observed in White of Dendennonde breed, while the New Zealand White rabbits showed only the Po S phenotype. The earlier reported rabbit serum protein alleles, PaF and Pas (post-albumin) and Prt + (pretransferrin) were shown to belong to the same genetic system and their new nomenclature is proposed to be PrtD, PrtF and PrtS, respectively. The three Prt alleles with considerable frequencies were observed in each of the two breeds studied.

Demonstration of serum albumin (ALB) polymorphism in wild rabbits, Oryctolagus cuniculus, by means of isoelectric focusing

“…Briefly, a solution containing polyacrylamide (T = 5 % , C = 3%), 60mM L-serine, 8 M urea and 6.25% (v/v) carrier ampholytes (1:l mixture of pH 5-6 and pH 5-8, Pharmacia) was poured in a 235 X 100 x 0.3mm cassette. The data obtained from screening of Portuguese domestic rabbits confirmed previous results on the absence of variation at this locus in several domestic breeds (Binette et al 1965;Zaragoza et al 1984;Juneja et al 1984). 4 4~ ammonium persulphate solution and 0.1% (vh) of TEMED.…”

“…The difficulties of performing controlled matings with wild rabbits prevent the presentation of family data. The data obtained from screening of Portuguese domestic rabbits confirmed previous results on the absence of variation at this locus in several domestic breeds (Binette et al 1965;Zaragoza et al 1984;Juneja et al 1984). On the contrary, the analysis of wild populations, both from Portugal and England, reveals a high degree of genetic diversity of ALB.…”

“…1965; Zaragoza et al 1984; Juneja et al 1984). These included the analysis of ALB by both one-and two-dimensional gel electrophoresis, using domestic breeds as a source of plasma samples, and failed to reveal any variation (Binette et af.…”

“…These included the analysis of ALB by both one-and two-dimensional gel electrophoresis, using domestic breeds as a source of plasma samples, and failed to reveal any variation (Binette et af. 1965; Zaragoza et al 1984; Juneja et al 1984).…”

Absence of genetic linkage between A1BG, PGD and HPX loci in rabbits

“…Genetic polymorphism of a rabbit serum pretransferrin (Prt) was reported by Usher et al (1983,1984) and by other workers (Juneja et al 1984;Varga et al 1986Varga et al ,1987. The Prts described by these different authors are very likely to be identical, but direct evidence is lacking.…”