Genetic polymorphism of Fcγ-receptors IIa, IIIa and IIIb in South Indian patients with generalized aggressive periodontitis

Abstract

Recent evidence suggests that polymorphisms in Fcγ receptors are associated with different forms of periodontitis. However, the FcγR genotypes and their allele frequency differ among subjects from different ethnic backgrounds. The aim of the present study was to determine whether specific FcγRIIa, FcγRIIIa, and FcγRIIIb alleles and/or genotypes are associated with susceptibility to generalized aggressive periodontitits (GAgP) in a South Indian population. Buccal scrapings were obtained from 60 subjects with GAgP and 60 periodontally healthy individuals, and DNA was extracted from each of the samples. FcγRIIa and FcγRIIIa genotyping was performed by polymerase chain reaction (PCR) amplification of DNA with allele-specific primers followed by allele-specific restriction digestion of the products, whereas FcγRIIIb genotyping was done by allele-specific PCR. There was no significant difference in the distribution of the FcγRIIa H/R genotype between GAgP patients and healthy subjects, although significant over-representation of the R allele was noted in GAgP patients. With regard to FcγRIIIa F/V genetic polymorphism, the homozygous V/V genotype and V allele were significantly over-represented in the GAgP group, whereas the F/F genotype and F allele were over-represented in the controls. Furthermore, there was significant over-representation of the FcγRIIIb-NA2 allele and NA2/NA2 genotype in GAgP patients, and of the NA1/NA1 genotype and NA1 allele in the controls. These data suggest that the FcγRIIIa V/V genotype and/or V allele, as well as the FcγRIIIb NA2/NA2 and/or NA2 allele, along with the FcγRIIa- R allele, may be risk factors for GAgP in the population of South India.

Polymorphic Regions in Fc Gamma Receptor and Tumor Necrosis Factor‐α Genes and Susceptibility to Chronic Periodontitis in a Cohort From South India

“…However, stratification by ethnicity revealed an association between the RR+RH genotype with periodontitis in a white population, but not in East Asian and African populations 36 . A recent study in patients with AgP from an ethnically similar population in South India reported a higher prevalence of the R allele in patients with AgP 10 . The present study has demonstrated a lack of association of FCGR2A His/Arg SNP with CP susceptibility in the 25‐ to 50‐year age cohort from South India.…”

“…Candidate gene studies evaluating association of the SNP in the FcGR gene complex with chronic periodontitis (CP) susceptibility have given inconclusive results 7–9 . Published literature on the role of SNP in FCGR2A , 2B , 3A , 3B and CP susceptibility in the Indian population is scant 10 …”

Understanding Inter-Individual Variability in Monoclonal Antibody Disposition

“…For example, the FcγRIIa-H131 genotype was found to be associated with an increased production of the pro-inflammatory cytokine IL-1β by mononuclear cells, leading to inter-individual differences in the risk for acquiring periodontitis [140]. In multiple studies carried out in diverse ethnic populations, significant associations were found between FcγR polymorphisms and inter-individual differences in susceptibility, prevalence, and prognosis of diseases like systemic lupus erythematosus (SLE) [141][142][143][144], rheumatoid arthritis (RA) [145,146], immune thrombocytopenia [147], Guillain-Barré syndrome [148][149][150], myasthenia gravis [151] pediatric autoimmune neutropenia [152], IgA nephropathy [153], inflammatory myopathies [154], anti-glomerular basement membrane antibody disease [155], Kawasaki Disease [156], periodontitis [157,158], malaria [159,160], dengue [161], and sickle cell disease [162].…”

Association of Fc gamma-receptors IIa, IIIa, and IIIb genetic polymorphism with susceptibility to chronic periodontitis in South Indian population

“…Although, NA1/NA2 polymorphism has been linked with GAgP, the studies on its association with GCP are limited. [ 18 ] Except for one study where FcγRIIIbNA2/NA2 was more prevalent in GCP patients,[ 30 ] none of the studies have found a relationship between this genotype and chronic periodontitis. [ 35 36 ] Our results also did not show any significant difference in the distribution of FcγRIIIb genotypes between GCP and controls.…”

“…In a similar study on genetic polymorphism of FcγRIIa, IIIa, and IIIb genes in South Indian population with generalized aggressive periodontitis (GAgP), it was concluded that FcγRIIIa V/V genotypes and/or V allele as well as FcγRIIIb NA2/NA2 and/or NA2 allele along with FcγRIIa R allele may be a risk factor for GAgP. [ 18 ]…”