Genetic Polymorphism in Species and Hybrids of Coffea Revealed by RAPD

Ruas, Paulo Maurício; Diniz, L. E. C.; Ruas, Claudete de Fátima; Sera, Tumoru

doi:10.1007/978-94-017-1068-8_16

Cited by 3 publications

(7 citation statements)

References 20 publications

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“…Coffea eugenioides was also relatively close to both N. 2 hybrid (0.67) and N. 5 hybrid (0.61). These results are in agreement with previous studies based on RAPD data (Ruas et al, 2000). Whereas N. 5 hybrid showed morphological patterns similar with C. arabica, N. 2 hybrid was morphologically very similar to hybrid N. 5.…”

Section: Parentage Identification Of Interspecific Hybridssupporting

confidence: 93%

“…dewevrei and C. eugenioides. The associations based on ISSR markers are also consistent with those obtained by RAPD (Ruas et al, 2000) suggesting that C. liberica var. dewevrei and C. eugenioides are the putative parents of the N. 4 hybrid.…”

Section: Parentage Identification Of Interspecific Hybridssupporting

confidence: 82%

“…However, Lashermes et al (1999) applying the RFLP and GISH clearly suggested that C. arabica is an amphidiploid derived from natural hybridization between C. eugenioides and C. canephora. Studies based on RAPD markers also placed C. arabica close to C. eugenioides, suggesting that this species is likely to be one of the parents of Arabic coffee (Ruas et al, 2000). The coefficients of genetic similarity placed C. canephora and C. eugenioides very close to C. arabica while C. congensis was less related with a similarity coefficient of 0.59, supporting previous studies and emphasizing the utility of ISSR polymorphism to deduce the origin of the arabica genome.…”

Section: Identification Of C Arabica Genomessupporting

confidence: 77%

“…robusta (0.30). Similar associations were observed with RAPD markers (Ruas et al, 2000) and with comparisons of ITS2 sequences (Lashermes et al, 1997). The low genetic similarity values identified with the ISSR markers (Table 3) could represent the actual genetic diversity among species and clearly demonstrate the usefulness of the technique in determining the interspecific genetic relationship in Coffea.…”

Section: Genetic Variation Among Coffea Species and Parentage Identifsupporting

confidence: 71%

“…dewevrei, C. stenophylla, and C. racemosa) showed low genetic similarities with N. 1 hybrid (Table 3, Figures, 1 and 2). Results of RAPD markers were not conclusive to define the origin of N. 1 hybrid (Ruas et al, 2000). The findings of ISSR markers clearly suggest that C. arabica and C. canephora are the best candidates as progenitors of N. 1 hybrid.…”

Section: Parentage Identification Of Interspecific Hybridsmentioning

confidence: 80%

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Genetic relationship in Coffea species and parentage determination of interspecific hybrids using ISSR (Inter- Simple Sequence Repeat) markers

Ruas

Rampim

et al. 2003

Genet. Mol. Biol.

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Inter-simple sequence repeat (ISSR) markers were used to evaluate genetic divergence among eight Coffea species and to identify the parentage of six interspecific hybrids. A total of 14 primers which contained different simple sequence repeats (SSR) were used as single primers or combined in pairs and tested for PCR amplifications. Two hundred and thirty highly reproducible fragments were amplified, which were then used to estimate the genetic similarity and to cluster the Coffea species and hybrids. High levels of interspecific genetic variation were revealed. The dinucleotide motif (GA) 9 T combined with other di-tri-and tetra-nucleotides produced a greater number of DNA fragments, mostly polymorphics, suggesting a high frequency of the poly GA microsatellite motifs in the Coffea genomes. The genetic similarity ranged from 0.25 between C. racemosa and C. liberica var. dewevrei to 0.86 between C. arabica var. arabica and Hybrid N. 2. The C. arabica species shared most of its markers with five of the six hybrids suggesting that it is the most likely candidate as one of the progenitors of those hybrids. These results revealed that ISSR markers could be efficiently used for genetic differentiation of the Coffea species and to identify the parentage of Coffea interspecific hybrids.

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Section: Parentage Identification Of Interspecific Hybridssupporting

confidence: 93%

Section: Parentage Identification Of Interspecific Hybridssupporting

confidence: 82%

Section: Identification Of C Arabica Genomessupporting

confidence: 77%

Section: Genetic Variation Among Coffea Species and Parentage Identifsupporting

confidence: 71%

Section: Parentage Identification Of Interspecific Hybridsmentioning

confidence: 80%

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Genetic relationship in Coffea species and parentage determination of interspecific hybrids using ISSR (Inter- Simple Sequence Repeat) markers

Ruas

Rampim

et al. 2003

Genet. Mol. Biol.

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Genetic diversity and structure of Ethiopian, Yemen and Brazilian Coffea arabica L. accessions using microsatellites markers

Silvestrini

Junqueira

Favarin

et al. 2007

Genet Resour Crop Evol

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Genetic diversity of a Coffea Germplasm Collection assessed by RAPD markers

Silvestrini

Maluf

Silvarolla

et al. 2007

Genet Resour Crop Evol

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Genetic diversity and relationships within and among nine species of Coffea, one species of Psilanthus and the Piatã hybrid from the Coffee Germplasm Collection of Instituto Agronô-mico de Campinas (IAC), Brazil were assessed using RAPD markers. Genetic diversity and relationships were evaluated by proportion of polymorphic loci (P), Shannon's genetic index (H 0 and G 0 ST ) and clustering analysis. The overall RAPD variation among all accessions was mostly partitioned between rather than within species. However, C. canephora and C. liberica showed a high genetic diversity within the species (H 0 sp = 0.414 and H 0 sp = 0.380, respectively) and this was highly structured (high G 0 ST ). Genetic diversity from C. congensis and C. arabica was also structured, but with lower levels of genetic diversity (H 0 sp = 0.218 and H 0 sp = 0.126, respectively). The results were consistent with agronomic and molecular studies and demonstrated that the IAC Coffea Collection is representative of the phylogenetic structure observed in the genera. This study devises sampling strategies for coffee germplasm collections and provides genetic diversity parameters for future comparisons among them.

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Genetic Polymorphism in Species and Hybrids of Coffea Revealed by RAPD

Cited by 3 publications

References 20 publications

Genetic relationship in Coffea species and parentage determination of interspecific hybrids using ISSR (Inter- Simple Sequence Repeat) markers

Genetic relationship in Coffea species and parentage determination of interspecific hybrids using ISSR (Inter- Simple Sequence Repeat) markers

Genetic diversity and structure of Ethiopian, Yemen and Brazilian Coffea arabica L. accessions using microsatellites markers

Genetic diversity of a Coffea Germplasm Collection assessed by RAPD markers

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