Genetic, phenotypic, and phytochemical polymorphism in Eastern European populations of Mentha arvensis L.

Shelepova, Olga; Семенова, М. В.; Енина, О. Л.; Schanzer, Ivan A.

doi:10.1134/s1022795416120139

Cited by 4 publications

(5 citation statements)

References 10 publications

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“…Plemenkov (2007). The qualitative composition of the oil was determined by gas chromatography in the "Biotechno logy" Shared Access Center, RAS (RFMEFI62114X0002) in a Shimadzu GS 2010 gas chromatograph with a GCMsQP 2010 mass detector as previously described in (Shelepova et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

“…Two series of PCR were carried out for 46 and 42 samples, and some of the samples were retested by PCR. ISSRPCR and the separation of PCR products were carried out by previously reported methods (Shelepova et al, 2016b(Shelepova et al, , 2017.…”

Section: Methodsmentioning

confidence: 99%

“…How ever, the resulting notion was still incomplete. A number of investigators successfully undertook a combined assessment of plants by morphological characteristics, essential oil com position, and ISSRanalysis data to study interspecies and interpopulation variation in the genus Mentha (Hua et al, 2011;Rodrigues et al, 2013;Shelepova et al, 2017). There fore, to accomplish our task; we comprehensively examined morphological features, essential oil compositions, and ISSR markers of DNA in two mint species.…”

Section: Introductionmentioning

confidence: 99%

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Intra- and interspecific variability of Mentha arvensis L. and M. Canadensis L.

2020

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The identification of plants of the genus Mentha is often difficult due to significant intraspecific polymorphism, intense interspecific hybridization, and ploidy changes. An attempt was made to apply an integrated approach to the study of different parameters of two species: Mentha arvensis L. and M. canadensis L. Eight geographically dispersed populations of Mentha in different regions (European Russia, Khakassia and Far East, Western Ukraine, and Indochina) were studied. Diagnostic morphological characters and compositions of essential oil components were examined, and DNA was analyzed with ISSR markers. The data obtained were statistically processed by cluster, principal component, and principal coordinate analyses. The European and Asian groups of samples were clearly distinguished by the analysis of quantitative parameters of the calyx and leaves, but different methods of data processing produced different results in determining the belonging of the Far Eastern plants to a particular group. Therefore, their taxonomic positions can hardly be determined on morphological grounds. According to the composition of essential oil and ISSR fragments, a group of the genetically, morphologically, and phytochemically closest plants was identified, which included representatives of the populations of the Moscow oblast, Vladimir oblast, Kaluga oblast, the Komi Republic, and Khakassia. All these plants belonged to M. arvensis. Plants collected in the natural flora of the Russian Far East showed a greater resemblance in essential oil composition and ISSR markers to the European group of M. arvensis than to plants from Indochina, which, according to the data obtained, belonged to M. сanadensis. It was shown that a comprehensive study of plant morphological characters, the compositions of essential oil, and ISSR fragments allows one to clarify the species identity and to assess their polymorphism and the degree of kinship between populations. A certain correlation between the data of molecular analysis and the composition of essential oil and, to a lesser extent, their correlation with morphological characters of plants was revealed.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Intra- and interspecific variability of Mentha arvensis L. and M. Canadensis L.

2020

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“…The qualitative composition of the oil was determined by gas chromatography at the Center for Collective Use of the Federal Research Center "Fundamentals of Biotechnology", (RFMEFI62114) (Moscow, Russian) [34]. The identification of essential oil components was obtained by gas chromatography-mass spectrometry (GC-MS).…”

Section: Isolation Of the Essential Oil And Determination Of Essential Oil Compositionmentioning

confidence: 99%

Chemical Components and Biological Activities of Essential Oils of Mentha × piperita L. from Field-Grown and Field-Acclimated after In Vitro Propagation Plants

et al. 2021

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In this work, we studied in vitro propagation of three cultivars of Mentha × piperita L. Murashige and Skoog medium (MS) supplemented with 0.5 mg·L−1 BAP was the most optimal medium for micropropagation of the cultivars studied. The ability of peppermint plants field-acclimated after in vitro micropropagation to produce essential oils (EOs) was investigated. EO was obtained by hydrodistillation from dried leaves and flowering shoots from control (field grown) plants and plants acclimated in field conditions after in vitro propagation. The samples were collected at the first and second year of vegetation, and their chemical composition was investigated using gas chromatography-mass spectrometry (GC-MS). Differences were observed in the yield, as well as in the quantitative and qualitative composition of the EOs extracted from the control plants and field-acclimated plants after in vitro propagation. Menthol was the main component of the EO in control plants, while pulegone and menthone were dominant in the EO pattern in field-acclimated in vitro regenerants in the first year of the growing season. However, in the second year of vegetation, the content of the main EO components in field-acclimated peppermint plants was approximately the same as in control plants. The antioxidant activity of EOs extracted from field-acclimated after in vitro micropropagation plants was found to be the same as in control field-grown M. × piperita plants.

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“…Few studies have been reported on the genetic variation in mint species worldwide especially in Iran (Khanuja et al, 2000;Shasany et al, 2005;Shelepova et al, 2017;Jedrzejczyk and Rewers 2018). Momeni and coworkers (2006), Kazemi and Hajizadeh (2012), and Taghiloofar and Anghourani (2014) evaluated the genetic diversity of 4, 1, and 2 mint species by RAPD markers in Iran, respectively, and suggested that RAPD markers are a suitable method for the evaluation of the genetic diversity of mint accessions.…”

Section: Introductionmentioning

confidence: 99%

Genetic relationships of Iranian endemic mint species, Mentha mozaffariani Jamzad and some other mint species revealed by ISSR markers

Choupani¹,

Shojaeiyan²,

Maleki³

2019

bta

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Mint species (Mentha sp.) are important vegetables with medicinal and economic characteristics. In this study, the genetic relationships of 34 accessions of 5 mint species including Mentha spicata, Mentha longifolia, Mentha piperita, Mentha aquatica, and Mentha mozaffarianii Jamzad were evaluated using Inter-Simple Sequence Repeat (ISSR) markers, followed by the analysis of the polymerase chain reaction (PCR) products on a high-resolution acrylamide gel. ISSR primers yielded 74 clear and reproducible bands, of which 50 bands were polymorph (65.28%), with a minimum of 7 ((TG) 8 G) and a maximum of 17 ((GA) 8 YC) bands per primer. Polymorphism information content (PIC) for each primer varied from 0.37 to 0.46 with an average of 0.41. The marker index referred to (GA) 8 YC primer as the most efficient compared to others. The analysis of the molecular variance (AMOVA) at the species level showed that variation within the species (75%) exhibits greater partitioning than the variation among species (15%). The cluster analysis performed was based on Nei's genetic distances using the unweighted pair group method with arithmetic mean (UPGMA) method. At the accession level, all 34 accessions were separated into 3 main clusters. The cluster analysis at the species level placed 3 species including M. aquatica, M. longifolia, and M. spicata in one common group. Based on the cluster analysis, the M. mozaffarianii Jamzad, as an Iranian species, was placed in Mentha section, closer to other species, when compared to M. piperita. The results indicated that ISSR markers alongside a high-resolution electrophoresis can be truly helpful in visualizing the diversity among different accessions of one species.

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Genetic, phenotypic, and phytochemical polymorphism in Eastern European populations of Mentha arvensis L.

Cited by 4 publications

References 10 publications

Intra- and interspecific variability of <i>Mentha arvensis</i> L. and <i>M. Canadensis</i> L.

Intra- and interspecific variability of <i>Mentha arvensis</i> L. and <i>M. Canadensis</i> L.

Chemical Components and Biological Activities of Essential Oils of Mentha × piperita L. from Field-Grown and Field-Acclimated after In Vitro Propagation Plants

Genetic relationships of Iranian endemic mint species, Mentha mozaffariani Jamzad and some other mint species revealed by ISSR markers

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