Genetic Origin of RD114 and Other RNA Tumour Viruses assayed by Molecular Hybridization

Gillespie, David; Gillespie, S.; Gallo, Robert C.; East, James L.; Dmochowski, L

doi:10.1038/newbio244051a0

Nature New Biology

1973

DOI: 10.1038/newbio244051a0

|View full text |Cite

Genetic Origin of RD114 and Other RNA Tumour Viruses assayed by Molecular Hybridization

David Gillespie

S. Gillespie

Robert C. Gallo

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1974

1982

Publication Types

Select...

Article7

Relationship

Self Cite0

Independent7

Authors

Journals

Cited by 48 publications

(29 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most other inbred strains of mice have a low and variable incidence of mammary tumors which occur late in life. Yet, like the high-incidence strains, they carry in their germ line multiple copies of the MMTV proviral genome (3)(4)(5)(6). The extent to which the genetically transmitted MMTV proviral genomes are involved in the development of spontaneous mammary tumors in low-incidence mouse strains varies with the strain in question.…”

mentioning

confidence: 99%

Novel class of mouse mammary tumor virus-related DNA sequences found in all species of Mus, including mice lacking the virus proviral genome.

Callahan¹,

Drohan²,

Gallahan³

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Mice in breeding colonies of feral Mus musculus brevirostris (Azrou, Morocco), M. m. musculus (Studenec, Czechoslovakia), and M. m. molossinus (Fukuoka, Japan) were found to lack the mouse mammary tumor virus (MMTV-a) proviral genome in their germ line. MMTV-a proviral genomes have been found in all inbred strains of M. musculus by using high-stringency nucleic acid hybridization conditions. We conclude that feral mice in these colonies are heterozygous for a limited number ofMMTVa proviral genomes and that those lacking them arose as a result ofrandom chromosomal segregation. All mice in another breeding colony of feral M. m. musculus (Sladeckovce, Czechoslovakia) lack MMTV proviral genes. By relaxing the conditions of nucleic acid hybridization, MMTV-related sequences (designated MMTV-fi) were detected in restricted cellular DNA from MMTV-negative mice and all other inbred strains and feral species of the genus Mus. The apparent ubiquity of the MMTV-,B DNA sequences in the genus Muw and the lack ofvariation in the pattern ofrestriction fragments containing these sequences within a species distinguishes them from MMTV-a. These results suggest that the MMTV-P DNA sequences either are the evolutionary progenitors of the infectious MMTV genome or represent an accumulation of evolutionarily divergent MMTV-a insertions into the mouse germ line.Many of the classical inbred strains of Mus musculus were intentionally derived from stocks that had a high incidence of mammary tumors (1). In many of these strains the high tumor incidence could be correlated with an infectious milk-borne mouse mammary tumor virus (MMTV) (2). Most other inbred strains of mice have a low and variable incidence of mammary tumors which occur late in life. Yet, like the high-incidence strains, they carry in their germ line multiple copies of the MMTV proviral genome (3-6). The extent to which the genetically transmitted MMTV proviral genomes are involved in the development of spontaneous mammary tumors in low-incidence mouse strains varies with the strain in question. The contribution of these viral genomes to tumor development in mice treated with exogenous carcinogens remains unresolved.Contributions from several laboratories have shown that each ofthe inbred strains ofmice that have been examined vary with respect to the number of MMTV proviral genomes in their germ line and their location in the cellular genome (7-9). Recently, the frequency and distribution of MMIV proviral genomes in cellular DNA from several populations of feral M. m.domesticus from California was examined (7). In general, these mice also exhibited great variability in number and location of the MMTV proviral genomes in their cellular DNA. Surprisingly, however, two mice were identified that had no MMTV proviral genome in their cellular DNA. These observations have led to the thesis that the genetically transmitted MMTV proviral genomes are the result of infrequent insertions of an infectious MMTV genome into the germ line during the evolution of the species (7)....

show abstract

mentioning

confidence: 99%

Novel class of mouse mammary tumor virus-related DNA sequences found in all species of Mus, including mice lacking the virus proviral genome.

Callahan¹,

Drohan²,

Gallahan³

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Unexpectedly, however, three of the secondary virus isolates additionally contained components related to the baboon endogenous virus (BaEV) ( 27,1975, a new sample of peripheral blood cells was obtained. Upon receipt, 2 g of these fresh uncultured HL23 blood cells were immediately homogenized in hypotonic buffer, and the nuclei and cytoplasm were separated by differential centrifugation (3); disposable or NaOH-treated glassware was used throughout.…”

mentioning

confidence: 99%

“…Sci. USA 73 (1976) ., enty-four percent of the HL23 cDNA hybridized to DNA 27. SiSV, simian sarcoma-leukemia virus; GaLV, gibbon ape leukemia virus; HL23V, virus from patient HL23.…”

mentioning

confidence: 99%

“…Unlabeled 70S viral RNA was purified according to Wu et al (26), except that the buffer used for glycerol gradient centrifugation was 0.1 M LiCl, 0.01 M Tris-HCI at pH 7.4, 0.001 M EDTA, and 0.2% sodium dodecyl sulfate. Cell DNA was purified (27); 3H-labeled 70S viral RNA was obtained (15,28); [3H]DNA copies of viral RNA (cDNA) were prepared under conditions specified earlier (3) and then were purified according to a modification of the procedure of Baxt and Spiegelman (5). The cDNA-containing solution was extracted with phenol-chloroform at pH 9 (27), and nucleic acids were made 0.2 M in NaCl and precipitated with 2 volumes of EtOH.…”

mentioning

confidence: 99%

“…Cell DNA was purified (27); 3H-labeled 70S viral RNA was obtained (15,28); [3H]DNA copies of viral RNA (cDNA) were prepared under conditions specified earlier (3) and then were purified according to a modification of the procedure of Baxt and Spiegelman (5). The cDNA-containing solution was extracted with phenol-chloroform at pH 9 (27), and nucleic acids were made 0.2 M in NaCl and precipitated with 2 volumes of EtOH. The precipitate was dissolved in 0.01 M Tris-HCl at pH 9, 0.5% sodium dodecyl sulfate, and 300 ,ug/ml of nuclease-free Pronase (Calbiochem) and incubated for 4 hr at 370.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Primate type-C virus nucleic acid sequences (woolly monkey and baboon types) in tissues from a patient with acute myelogenous leukemia and in viruses isolated from cultured cells of the same patient.

Reitz¹,

Miller²,

Wong‐Staal³

et al. 1976

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Cultured peripheral blood leukocytes from a woman (patient HL23) with acute myelogenous leukemia produced type-C RNA tumor viruses (HL23V). The viruses were analyzed by molecular hybridization experiments after transmission to five secondary cell culture lines. Using the criteria of molecular hybridization, we concluded that al of the transmitted virus isolates have nucleotide sequences related to the genome of simian sarcoma virus (SiSV). In addition, in agreement with data reported elsewhere, some of the transmitted viruses also have nucleotide sequences related to those of the baboon endogenous virus (BaEV).We also used molecular hybridization to ascertain whether both viruses could have originated from the patient HL23.Utilizing [3H]cDNA complementary to RNA from the separated

show abstract

Multiple childhood osteosarcomas in an american indian family with erythroid macrocytosis and skeletal anomalies

Mulvihill¹,

Gralnick²,

Whang‐Peng³

et al. 1977

Cancer

View full text Add to dashboard Cite

Three of nine children of possibly consanguineous American Indian parents developed typical osteosarcoma in a 2‐year period. Etiologic investigations detected limb anomalies and elevated mean corpuscular volumes (98‐109 μm3) in the surviving tumor patient, several of her sibs, and her father. Limb anomalies included simple clinodactyly with brachymesophalangy, absence of one digital ray of the foot, and bilateral radioulnar synostosis. The red cell macrocytosis was not accompanied by anemia or explained by the usual causes. No unusual environmental exposures were found and screening for possible oncogenic viruses by culture, electron microscopy, and serology was negative. All family members had elevated antibody titers to Epstein‐Barr viral antigens. The proband and her father had excessive chromosomal breaks in the bone marrow. This unusual familial pattern of osseous malignancy and malformation and defective erythropoiesis, tentatively called OSLAM syndrome, may represent impaired regulation of bone development. Cancer 40:3115‐3122, 1977.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Genetic Origin of RD114 and Other RNA Tumour Viruses assayed by Molecular Hybridization

Cited by 48 publications

References 22 publications

Novel class of mouse mammary tumor virus-related DNA sequences found in all species of Mus, including mice lacking the virus proviral genome.

Novel class of mouse mammary tumor virus-related DNA sequences found in all species of Mus, including mice lacking the virus proviral genome.

Primate type-C virus nucleic acid sequences (woolly monkey and baboon types) in tissues from a patient with acute myelogenous leukemia and in viruses isolated from cultured cells of the same patient.

Multiple childhood osteosarcomas in an american indian family with erythroid macrocytosis and skeletal anomalies

Contact Info

Product

Resources

About