Primate type-C virus nucleic acid sequences (woolly monkey and baboon types) in tissues from a patient with acute myelogenous leukemia and in viruses isolated from cultured cells of the same patient.

Abstract

Cultured peripheral blood leukocytes from a woman (patient HL23) with acute myelogenous leukemia produced type-C RNA tumor viruses (HL23V). The viruses were analyzed by molecular hybridization experiments after transmission to five secondary cell culture lines. Using the criteria of molecular hybridization, we concluded that al of the transmitted virus isolates have nucleotide sequences related to the genome of simian sarcoma virus (SiSV). In addition, in agreement with data reported elsewhere, some of the transmitted viruses also have nucleotide sequences related to those of the baboon endogenous virus (BaEV).We also used molecular hybridization to ascertain whether both viruses could have originated from the patient HL23.Utilizing [3H]cDNA complementary to RNA from the separated

Viruses and lymphocytes in rheumatoid arthritis. I. Studies on cultured rheumatoid lymphocytes.

“…Several isolations have been made, one of which was from cultured myeloid leukaemia cells. This virus was closely related to simian sarcoma virus and baboon endogenous virus (Reitz et al, 1976), and it can be established as a chronic infection in various cell lines (Teich et al, 1975).…”

Simian-Sarcoma-Virus-Related Sequences in DNAs from Human Prostatic Tissues

“…How ever, normal tissue DNA did contain sequences distantly related to baboon endogenous virus [11]. These work ers reportedly failed to detect an appreciable amount of sequences related to simian sarcoma virus in DNA from normal or neoplastic tissues [7,11,12]. The HL23 virus contained sequences indistinguishable from baboon endogenous virus as well as simian sarcoma virus [13], Paradoxically, simian-sarcoma-virus-related sequences were not detected even in DNA from the tissues of the patient from whose leukocytes HL23 virus was originally isolated [3,11].…”

“…Using complementary DNA (cDNA) prepared from oncornavirus-like cyto plasmic particles from human leukemic cells, Baxt and Spiegelman [10] reported that DNA from human leukemic cells contains particle-related sequences that were absent in DNA from leukocytes of normal individ uals. More recently, Reitz et al [7] and Wong-Staal et al [11] have detected in DNA from some human hematopoietic neoplastic tissues sequences related to baboon endogenous virus, murine leukemia virus 1 This work was supported in part by United States Public Health Service grant CA-15502 from the National Cancer Institute.…”

“…[Rauscher; 12] and a virus (HL23) isolated from cultures of human myelogenous leukemia cells [7], The probes generated from these viruses hybridized to DNA from neoplastic tissues to an extent significantly greater than to DNA from the normal tissues [7,11,12]. How ever, normal tissue DNA did contain sequences distantly related to baboon endogenous virus [11].…”

Type C RNA virus expression in systemic lupus erythematosus. New Zealand mouse model and human disease

“…Virus isolates claimed to be candidate human type C viruses have been described which are related t o infectious primate type C virus (29)(30)(31) or to both infectious and endogenous primate type C viruses (32). T h e expression of p30 protein antigen related to baboon endogenous virus has been reported for normal tissues of the baboon and for some neoplastic tissues of the baboon a n d man ( I 1,13).…”