Genetic networks of cooperative redox regulation of osteopontin

Partridge, Charles; He, Qing-Yu; Brun, Marcel; Ramos, Kenneth S.

doi:10.1016/j.matbio.2008.01.009

Cited by 7 publications

(4 citation statements)

References 59 publications

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“…Previous studies have shown that oxidative stress suppresses osteogenic differentiation in various cellular systems [35]–[37]. Indeed, regulatory transcription networks between redox-responsive elements and the OPN gene have been reported [38]. In this respect, the antioxidant activity of hesperetin could be linked to its osteogenic capability, suggesting that use of this compound may be a novel strategy to prevent the inhibitory effect of oxidative stress on osteogenesis of PDLSCs in a diabetic state.…”

Section: Discussionmentioning

confidence: 96%

Hesperetin Alleviates the Inhibitory Effects of High Glucose on the Osteoblastic Differentiation of Periodontal Ligament Stem Cells

et al. 2013

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Hesperetin (3′,5,7-trihydroxy-4-methoxyflavanone) is a metabolite of hesperidin (hesperetin-7-O-rutinoside), which belongs to the flavanone subgroup and is found mainly in citrus fruits. Hesperetin has been reported to be an effective osteoinductive compound in various in vivo and in vitro models. However, how hesperetin effects osteogenic differentiation is not fully understood. In this study, we investigated the capacity of hesperetin to stimulate the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) and to relieve the anti-osteogenic effect of high glucose. Osteogenesis of PDLSCs was assessed by measurement of alkaline phosphatase (ALP) activity, and evaluation of the mRNA expression of ALP, runt-related gene 2 (Runx2), osterix (OSX), and FRA1 as osteogenic transcription factors, as well as assessment of protein expression of osteopontin (OPN) and collagen type IA (COLIA). When PDLSCs were exposed to a high concentration (30 mM) of glucose, osteogenic activity decreased compared to control cells. Hesperetin significantly increased ALP activity at doses of 1, 10, and 100 µM. Pretreatment of cells with hesperetin alleviated the high-glucose-induced suppression of the osteogenic activity of PDLSCs. Hesperetin scavenged intracellular reactive oxygen species (ROS) produced under high glucose condition. Furthermore, hesperetin increased the activity of the PI3K/Akt and β-catenin pathways. Consistent with this, blockage of Akt or β-catenin diminished the protective effect of hesperetin against high glucose-inhibited osteogenic differentiation. Collectively, our results suggest that hesperetin alleviates the high glucose-mediated suppression of osteogenic differentiation in PDLSCs by regulating ROS levels and the PI3K/Akt and β-catenin signaling pathways.

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Section: Discussionmentioning

confidence: 96%

Hesperetin Alleviates the Inhibitory Effects of High Glucose on the Osteoblastic Differentiation of Periodontal Ligament Stem Cells

et al. 2013

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“…This effect of Hp could be linked to its antioxidant properties. Indeed, redox-responsive elements in the promoter of the OPN gene has been reported [66]. Moreover, it is possible that the longer time period of Hp treatment at these doses should be required to influence mineralization up to crystallization.…”

Section: Effect Of Hp On Osteoblast Functionsmentioning

confidence: 99%

Hesperetin stimulates differentiation of primary rat osteoblasts involving the BMP signalling pathway

Trzeciakiewicz

Habauzit

Mercier

et al. 2010

The Journal of Nutritional Biochemistry

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“…This evidence suggests that arginase activation reduces arginine bioavailability, thus NOS-mediated NO production, fundamental to maintain the endothelial function. Of notice, it has been reported a possible interaction between OPN and arginase ( Partridge et al, 2008 ). Thus, it is likely that OPN could stimulate arginase activity in the CAD context.…”

Section: Discussionmentioning

confidence: 99%

Relationship Between Plasma Osteopontin and Arginine Pathway Metabolites in Patients With Overt Coronary Artery Disease

et al. 2020

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Introduction Osteopontin (OPN) is involved in ectopic calcification. Its circulating form is upregulated in coronary artery disease (CAD) patients. Circulating OPN levels positively correlate with oxidative stress, one of the major triggers of endothelial dysfunction. Endothelial dysfunction is, in turn, associated with reduced nitric oxide (NO) bioavailability due to the impaired arginine pathway. The aim of this study was to better understand the correlations between OPN, oxidative stress markers, and the arginine pathway metabolites. Methods and Results ELISA and mass spectrometry techniques have been used to evaluate circulating OPN and arginine pathway/oxidative stress metabolites, respectively, in twenty-five control subjects and thirty-three patients with overt atherosclerosis. OPN positively correlates with 2,3-dinor-8isoPGF2a levels ( p = 0.02), ornithine ( p = 0.01), ADMA ( p = 0.001), SDMA ( p = 0.03), and citrulline ( p = 0.008) levels only in CAD patients. In addition, citrulline positively correlated with ADMA ( p = 0.02) levels, possibly as result of other sources of citrulline biosynthetic pathways. Conclusion The association between OPN and impaired arginine/NO pathway could play a role in the inhibition of endothelial NO synthase (eNOS) and/or in the arginase activation in the context of CAD patients. However, further studies are needed to verify the cause-effect relationship between OPN, oxidative stress, and arginine/NO pathway dysregulation.

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Genetic networks of cooperative redox regulation of osteopontin

Cited by 7 publications

References 59 publications

Hesperetin Alleviates the Inhibitory Effects of High Glucose on the Osteoblastic Differentiation of Periodontal Ligament Stem Cells

Hesperetin Alleviates the Inhibitory Effects of High Glucose on the Osteoblastic Differentiation of Periodontal Ligament Stem Cells

Hesperetin stimulates differentiation of primary rat osteoblasts involving the BMP signalling pathway

Relationship Between Plasma Osteopontin and Arginine Pathway Metabolites in Patients With Overt Coronary Artery Disease

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