Genetic mutations in adaptive evolution of growth-independent vancomycin-tolerant <i>Staphylococcus aureus</i>

Liu, Pilong; Hao, Zehua; Liu, Miaomiao; Niu, Mingze; Sun, Peng; Yan, Shunhua; Zhao, L L; Zhao, Xin

doi:10.1093/jac/dkab260

Cited by 8 publications

(6 citation statements)

References 60 publications

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“…There were no vancomycin-resistant isolates, but in recent years, a heterogeneous vancomycin-resistant S. aureus was found clinically. 28 Therefore, the clinical use of vancomycin should be carefully selected according to the patient’s drug sensitivity results to prevent the emergence of VRSA (vancomycin-resistant staphylococcus aureus ) in China.…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology of Staphylococcus aureus in a Tertiary Hospital in Anhui, China: ST59 Remains a Serious Threat

Zhang¹,

Cao²,

He³

et al. 2023

IDR

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This study aimed to investigate the molecular characteristics, antimicrobial resistance and hemolytic phenotype of Staphylococcus aureus isolated from Anhui, China. Results: From August 2021 to January 2022, 214 S. aureus isolates were collected from the Anhui Provincial Hospital. This study identified 117 methicillin-resistant S. aureus and 97 methicillin-sensitive S. aureus isolates, and the detection rate of methicillinresistant isolates was 1.8-fold higher than the average isolates reported in China (53.9% vs 30.5%). S. aureus isolates share identity at five or more of the seven MLST-based housekeeping loci, referred to as the clonal complex (CC). Forty ST types were found in 214 clinical S. aureus isolates, with the most extensive distribution of ST59 and ST6697 typing numbers and higher CC5 detection rates than any other clonal group. (The ST typing is the result of the MLST typing website query.) To detect the virulence of ST types of S. aureus, hemolysis experiments were performed on 214 clinical isolates, and it was concluded that ST59 had a relatively robust hemolytic capacity. Conclusion: Anhui S. aureus isolates have unique molecular and antibiotic resistance profiles. The antibiotic resistance profile may be related to the random use of antibiotics.

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Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology of Staphylococcus aureus in a Tertiary Hospital in Anhui, China: ST59 Remains a Serious Threat

Zhang¹,

Cao²,

He³

et al. 2023

IDR

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“…At present, functional research on the serine protease of S. aureus has been carried out. In the study of vancomycin resistant S. aureus (VTSA) strains, pbp4, htrA, stp1, pth and NWMN 1068 plays a role in the formation of VTSA [27]. The decrease in hemolytic capacity of strain SA113 treated with chloramphenicol may be due to the inhibition of agr quorum sensing system by chloramphenicol, thereby reducing SspA [28].…”

Section: Molecular Evolution Of Serine Proteases Genes Expanding In W...mentioning

confidence: 99%

Species-Specific Gene Expansion and Expressional Regulation of the Serine Protease Gene Family in Staphylococcus aureus from chronic wounds

Cao

Shi

Zhao

et al. 2023

Preprint

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Background Chronic wounds caused by pressure ulcers, diabetes foot ulcers, lower extremity arteriovenous ulcers, etc., not only increase the pain and mortality of patients, but also increase the social medical burden. The formation of bacterial biofilm is an important reason for the difficulty in healing chronic wounds. Staphylococcus aureus biofilm is an important pathogenic factor leading to the spread of infection in chronic wounds.Results Through comparative genomic analysis of S. aureus isolated from wounds and environmental samples, it was found that the unique genes of the wound isolate were enriched in the serine type peptidase activity, as well as metabolic pathways such as quorum sensing, signal transduction, and two component system. According to the analysis of the serine protease family of S. aureus, it was found that most of S. aureus strains isolated from the wound contains six serine protease-like proteins (SplA, SplB, SplC, SplD, SplE, SplF). The Staphylococcus phase UPMK1 may horizontally transfer the six serine protease-like protein genes to S. aureus in wounds and spread within the species. Through the transcriptome analysis of wild strain JE2 and its gltS knockout strains, it was found that the expression of serine protease genes (S1C, SspA, SplA and SplF) was significantly up-regulated when gltS mutant strains were cultured for 48 h in biofilm formation, which may play an important role in biofilm formation and pathogenicity.Conclusions Staphylococcus phase expand the genome of S. aureus in wounds. The spl serine protease gene of the S. aureus strain from the wound was obtained through horizontal gene transfer. The significant upregulation of gene expression of serine proteases (S1C, SspA, SplA, and SplF) is closely related to the formation of wound biofilm of S. aureus.

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“…and Unicycler v0.4.6 for de novo assembly and obtaining of the complete genome sequence (34). Gene annotations were carried out with the RAST online tool (35) Construction of recombinant strains Construction of recombinant plasmids for gene complementation was performed as described previously (41). The target gene was amplified by PCR and inserted into the shuttle vector pBBR1MCS-3(see Table S1 in Supplemental materials for primer).…”

Section: Whole Genome Sequencing(wgs) and Single Nucleotide Polymorph...mentioning

confidence: 99%

Co-occurrence of ST412Klebsiella pneumoniaeisolates with hypermucoviscous and no-mucoviscous phenotypes in a short-term hospitalized patient

Liang,

Zhang,

Wang

et al. 2023

Preprint

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Hypermucoviscosity(HMV) is a phenotype that is commonly associated with hypervirulence inKlebsiella pneumoniae. The factors that contribute to the emergence of HMV subpopulations remain unclear. In this study, eightK. pneumoniaestrains were recovered from an inpatient who were hospitalized for 20 days. Three of the isolates exhibited a non-HMV phenotype, which was concomitant with increased biofilm formation and higher siderophore secretion than the other five HMV isolates. All eight isolates were highly susceptible to serum killing, albeit HMV strains were remarkably more infective than non-HMV counterparts in a mouse model of infection. Whole genome sequencing(WGS) showed that the eight isolates belonged to the K57-ST412 lineage. Average nucleotide identity(ANI) analysis indicated that eight isolates share 99.96% to 99.99% similarity and were confirmed to be the same clone. Through comparative genomics analysis, 12 non-synonymous mutations were found among these isolates, seven of which in the non-HMV variants, includingrmpA(R96G) andwbap(S435R), which are assumed to be associated with the non-HMV phenotype. The mutationsmanB(G440L),dmsB(R193W) andtkt(A643N) occurred in HMV isolates only. RNA-Seq and RT-qPCR revealed transcripts of genes involved in transporter activity, carbohydrate metabolism and energy metabolism, includingcysK,paaF,vasD,celCandfruA, to be significantly dysregulated in the non-HMV strain K201060 compared to the HMV strain K201059, suggesting a participation in HMV phenotype development. This study suggests that co-occurrence of HMV and non-HMV phenotypes in the same clonal population may be mediated by mutational mechanisms as well as by certain genes involved in transport and central metabolism.

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Genetic mutations in adaptive evolution of growth-independent vancomycin-tolerant Staphylococcus aureus

Cited by 8 publications

References 60 publications

Molecular Epidemiology of Staphylococcus aureus in a Tertiary Hospital in Anhui, China: ST59 Remains a Serious Threat

Molecular Epidemiology of Staphylococcus aureus in a Tertiary Hospital in Anhui, China: ST59 Remains a Serious Threat

Species-Specific Gene Expansion and Expressional Regulation of the Serine Protease Gene Family in Staphylococcus aureus from chronic wounds

Co-occurrence of ST412Klebsiella pneumoniaeisolates with hypermucoviscous and no-mucoviscous phenotypes in a short-term hospitalized patient

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