The adipocyte-derived adiponectin hormone bridges obesity and its cardio-metabolic complications. Genetic variants at the
ADIPOQ
locus, in
ADIPOR1
, and
ADIPOR2
have been associated with adiponectin concentrations and cardio-metabolic complications in diverse ethnicities. However, no studies have examined these associations in Mexican children. We recruited 1 457 Mexican children from Mexico City. Six genetic variants in or near
ADIPOQ
(rs182052, rs2241766, rs266729, rs822393),
ADIPOR1
(rs10920533), and
ADIPOR2
(rs11061971) were genotyped. Associations between serum adiponectin, genetic variants, and cardio-metabolic traits were assessed using linear and logistic regressions adjusted for age, sex, and recruitment center. Serum adiponectin concentration was negatively associated with body mass index, waist to hip ratio, low-density lipoprotein cholesterol, total cholesterol, triglycerides, fasting glucose, fasting insulin, homeostatic model assessment of insulin resistance, dyslipidemia and overweight/obesity status (7.76 × 10
−40
≤ p ≤ 3.00 × 10
−3
). No significant associations between genetic variants in
ADIPOQ
,
ADIPOR1
, and
ADIPOR2
and serum adiponectin concentration were identified (all p ≥ 0.30). No significant associations between the six genetic variants and cardio-metabolic traits were observed after Bonferroni correction (all p < 6.9 × 10
−4
). Our study suggests strong associations between circulating adiponectin concentration and cardio-metabolic traits in Mexican children.