Genetic Markers for the Analysis of Variability and for Production of Specific Diagnostic Sequences in Fumonisin-Producing Strains of Fusarium Verticillioides

González-Jaén, M. Teresa; Mirete, Salvador; Patiño, Belén; López‐Errasquín, Elena; Vázquez, Covadonga

doi:10.1023/b:ejpp.0000032392.20106.81

Cited by 55 publications

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“…Similar results were reported for F. proliferatum strains isolated from date palm and banana, even when based on the analysis of the tef -1α gene, but using a significantly longer fragment of the gene (Jurado et al 2010). On the other hand, genes from the FUM cluster (particularly FUM1 ) were already accepted as good targets for phylogenetic studies and also for designing markers useful in the diagnostics of the fumonisin production ability of species from the G. fujikuroi species complex (Baird et al 2008; von Bargen et al 2009; González-Jaén et al 2004), as well as for distinguishing individual species belonging to the G. fujikuroi species complex (Stępień et al 2011). Of all of the isolates we used in the FUM1 analysis, the best distinguished group consisted of all six strains originating from garlic ( Allium sativum L.).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, genes and other sequences directly involved in secondary metabolism gained more attention in phylogenetic studies, as those have the advantage of possible usage in combined approaches to the diagnostics of mycotoxin production abilities (Proctor et al 2009). Therefore, genes from the FUM cluster merit investigation as a good additional marker for phylogenetic studies of fumonisin-producing Fusarium species (Baird et al 2008; González-Jaén et al 2004; Stępień et al 2011). …”

Section: Introductionmentioning

confidence: 99%

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Genetic and phenotypic variation of Fusarium proliferatum isolates from different host species

2011

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Fusarium proliferatum (Matsushima) Nirenberg is a common pathogen infecting numerous crop plants and occurring in various climatic zones. It produces large amounts of fumonisins, a group of polyketide-derived mycotoxins. Fumonisin biosynthesis is determined by the presence and activity of the FUM cluster, several co-regulated genes with a common expression pattern. In the present work, we analyzed 38 F. proliferatum isolates from different host plant species, demonstrating host-specific polymorphisms in partial sequences of the key FUM1 gene (encoding polyketide synthase). We also studied growth rates across different temperatures and sample origin and tried to establish the relationships between DNA sequence polymorphism and toxigenic potential. Phylogenetic analysis was conducted based on FUM1 and tef-1α sequences for all isolates. The results indicated the greatest variations of both toxigenic potential and growth patterns found across the wide selection of isolates derived from maize. Fumonisin production for maize isolates ranged from 3.74 to 4,500 μg/g of fumonisin B1. The most efficient producer isolates obtained from other host plants were only able to synthesize 1,820–2,419 μg/g of this metabolite. A weak negative rank correlation between fumonisin content and isolate growth rates was observed. All garlic-derived isolates formed a distinct group on a FUM1-based dendrogram. A second clade consisted of tropical and sub-tropical strains (isolated from pineapple and date palm). Interestingly, isolates with the fastest growth patterns were also grouped together and included both isolates originating from rice. The sequence of the FUM1 gene was found to be useful in revealing the intraspecific polymorphism, which is, to some extent, specifically correlated with the host plant.Electronic supplementary materialThe online version of this article (doi:10.1007/s13353-011-0059-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic and phenotypic variation of Fusarium proliferatum isolates from different host species

2011

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“…The high variability provided by these regions is particularly useful when it is necessary to discriminate among closely related species or at intraspecific level. Both ITS and IGS regions have been used to carry out phylogenetic and population studies in filamentous fungi [22][23][24][25][26][27] and to develop specific PCR assays to identify important mycotoxigenic species affecting commodities, such as Fusarium or Aspergillus [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Discrimination of Aspergillus niger and other Aspergillus species belonging to section Nigri by PCR assays

González-Salgado

Patiño²,

Vázquez

et al. 2005

FEMS Microbiology Letters

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Aspergillus species included in section Nigri are common in plant products and processed food, such as grapes, cereals, coffee and derivatives, particularly in warm and tropical climates. Two of these species, A. carbonarius and A. niger, are known to produce ochratoxin A (OTA), a potent nephrotoxin and carcinogenic to human (group 2B). Recognition of the several species of this section is difficult and requires considerable expertise using conventional methods based on morphological features. In this work we describe rapid, sensitive and robust assays based on the PCR technique to discriminate the main species included in section Nigri: A. japonicus, A. heteromorphus, A. ellipticus and the two morphologically indistinguishable species of the A. niger aggregate: A. niger and A. tubingensis. The species-specific primers have been designed on the basis of ITS (internal transcribed spacers of rDNA units) sequence comparisons obtained from several Aspergillus strains and have been tested in a number of strains from different origins and hosts. These PCR assays, based on multi-copy sequences, are highly sensitive and specific and represent a good tool for an early detection of OTA-producing Aspergillus species in order to prevent OTA from entering the food chain.

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“…The following reference strains were used in the analyses as positive and negative controls: F. verticillioides A149 (Department of Plant Pathology, Kansas State University, Manhattan, Kansas); Fusarium thapsinum M6561 (Fusarium Research Center, Department of Plant Pathology, The Pennsylvania State University, University Park, Pennsylvania); and Fusarium proliferatum MRC 8550 (PROMEC Unit of the South African Medical Research Council, Tygerberg, South Africa). Previous analysis of the F. thapsinum strains has shown that they do not possess the FUM1 gene González-Jaén et al 2004). The F. proliferatum reference strain was used as a negative control in the amplifications with the VERT-1 and VERT-2 primers.…”

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confidence: 99%

Sequence variability in the FUM1 gene of Fusarium verticillioides strains

Silva

Araújo²,

Durigon³

et al. 2007

Can. J. Microbiol.

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Fumonisins are mycotoxins, produced mainly by Fusarium verticillioides, that are potentially carcinogenic to humans and toxic to animals. Synthesis of these toxins is directed by a cluster of 15 genes, among which FUM1 is the largest; it encodes a polyketide synthase. This enzyme probably catalyzes the synthesis of a polyketide that forms a large portion of the fumonisin structure. In this study, 27 strains possessing the FUM1 gene, as determined by polymerase chain reaction, were analyzed. A portion of the FUM1 gene was amplified and sequenced from 6 of 27 Brazilian strains isolated from corn and sorghum. The sequence similarity for the six F. verticillioides strains was almost 100%.

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Genetic Markers for the Analysis of Variability and for Production of Specific Diagnostic Sequences in Fumonisin-Producing Strains of Fusarium Verticillioides

Cited by 55 publications

References 0 publications

Genetic and phenotypic variation of Fusarium proliferatum isolates from different host species

Genetic and phenotypic variation of Fusarium proliferatum isolates from different host species

Discrimination of Aspergillus niger and other Aspergillus species belonging to section Nigri by PCR assays

Sequence variability in the FUM1 gene of Fusarium verticillioides strains

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