Discrimination of Aspergillus niger and other Aspergillus species belonging to section Nigri by PCR assays

González-Salgado, Amaia; Patiño, Belén; Vázquez, Covadonga; González-Jaén, M. Teresa

doi:10.1016/j.femsle.2005.03.023

Cited by 59 publications

(33 citation statements)

References 33 publications

(38 reference statements)

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“…5) is classified as a ubiquitous nephrotoxic mycotoxin and it was reported to be responsible for human Balkan endemic nephropathy and involved in urinary tract tumors. For this reason, it was classified by the Agency for Research on Cancer as a possible carcinogen belonging to group 2B (168). Ochratoxin A has a genotoxic activity on the cell DNA followed by oxidative metabolism, which plays a major role in Ochratoxin mediated carcinogenesis.…”

Section: Mycotoxins and Their Carcinogenicitymentioning

confidence: 99%

Effects of different mycotoxins on humans, cell genome and their involvement in cancer

et al. 2017

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Abstract. The chemical nature of most of the mycotoxins makes them highly liposoluble compounds that can be absorbed from the site of exposure such as from the gastrointestinal and respiratory tract to the blood stream where it can be dissimilated throughout the body and reach different organs such as the liver and kidneys. Mycotoxins have a strong tendency and ability to penetrate the human and animal cells and reach the cellular genome where it causes a major mutagenic change in the nucleotide sequence which leads to strong and permanent defects in the genome. This defect will eventually be transcribed, translated and lead to the development of cancer. In this review, the chemical and physical nature of mycotoxins, the action of mycotoxins on the cellular genome and its effect on humans, mycotoxins and their carcinogenicity and mycotoxins research gaps are discussed, and new research areas are suggested. The research review posed various questions. What are the different mycotoxins that can cause cancer, what is the role of mycotoxins in causing cancer and what types of cancers can be caused by mycotoxins? These questions have been selected due to the significant increase in the mycotoxin contamination and the cancer incidence rate in the contemporary world. By revealing and understanding the role of mycotoxins in developing cancer, measures to reduce the risks and incidents of cancer could be taken.

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Section: Mycotoxins and Their Carcinogenicitymentioning

confidence: 99%

Effects of different mycotoxins on humans, cell genome and their involvement in cancer

et al. 2017

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“…Polymorphism within the ITS region is generally (but not always) at the level of species, rather than between isolates of the same species, making it an ideal target for the development of species-specific PCR assays. For example the species-specific primers have been designed on the basis of ITS sequence to discriminate the main species included in section Nigri: A. japonicus , A. heteromorphus , A. ellipticus and the two morphologically indistinguishable species of the A. niger aggregate: A. niger and A. tubingensis [190]. Other species-specific PCR-based assays to ochratoxigenic species were developed for A. carbonarius and A. ochraceus based on ITS [191].…”

Section: Detection Of Ochratoxigenic Species By Pcrmentioning

confidence: 99%

Ochratoxin A: General Overview and Actual Molecular Status

Khoury

Atoui

2010

Toxins

364

247

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Ochratoxin A (OTA) is a mycotoxin produced by several species of Aspergillus and Penicillium fungi that structurally consists of a para-chlorophenolic group containing a dihydroisocoumarin moiety that is amide-linked to L-phenylalanine. OTA is detected worldwide in various food and feed sources. Studies show that this molecule can have several toxicological effects such as nephrotoxic, hepatotoxic, neurotoxic, teratogenic and immunotoxic. A role in the etiology of Balkan endemic nephropathy and its association to urinary tract tumors has been also proved. In this review, we will explore the general aspect of OTA: physico-chemical properties, toxicological profile, OTA producing fungi, contaminated food, regulation, legislation and analytical methods. Due to lack of sufficient information related to the molecular background, this paper will discuss in detail the recent advances in molecular biology of OTA biosynthesis, based on information and on new data about identification and characterization of ochratoxin biosynthetic genes in both Penicillium and Aspergillus species. This review will also cover the development of the molecular methods for the detection and quantification of OTA producing fungi in various foodstuffs.

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“…Both ITS1 and ITS2 regions (Internal Transcribed Spacers of the rDNA) are highly variable, allowing discrimination of closely related species (Edwards et al 2002) and they have been successfully used to detect and identify other mycotoxigenic Aspergillus species (Henry et al 2000;Parenicova´et al 2001;Zhao et al 2001;Varga et al 2004;Gonza´lez-Salgado et al 2005;Patin˜o et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Specific detection ofAspergillus parasiticusin wheat flour using a highly sensitive PCR assay

Sardiñas

Vázquez

Gil-Serna

et al. 2010

Food Additives & Contaminants: Part A

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Aspergillus parasiticus is one of the most important aflatoxin-producing species that contaminates foodstuffs and beverages for human consumption. In this work, a specific and highly sensitive PCR protocol was developed to detect A. parasiticus using primers designed on the multicopy internal transcribed region of the rDNA unit (ITS1-5.8S-ITS2 rDNA). The assay proved to be highly specific for A. parasiticus when tested on a wide range of related and other fungal species commonly found in commodities, and allowing discrimination from the closely related A. flavus. Accuracy of detection and quantification by conventional PCR were tested with genomic DNA obtained from wheat flour artificially contaminated with spore suspensions of known concentrations. Spore concentrations equal or higher than 10(6) spore/g could be detected by the assay directly without prior incubation of the samples. The assay described is suitable for incorporation in routine analyses at critical points of the food chain within HACCP strategies.

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Discrimination of Aspergillus niger and other Aspergillus species belonging to section Nigri by PCR assays

Cited by 59 publications

References 33 publications

Effects of different mycotoxins on humans, cell genome and their involvement in cancer

Effects of different mycotoxins on humans, cell genome and their involvement in cancer

Ochratoxin A: General Overview and Actual Molecular Status

Specific detection ofAspergillus parasiticusin wheat flour using a highly sensitive PCR assay

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