Genetic Map Construction and Detection of Genetic Loci Underlying Segregation Distortion in an Intraspecific Cross of Populus deltoides

Abstract

Based on a two-way pseudo-testcross strategy, high density and complete coverage linkage maps were constructed for the maternal and paternal parents of an intraspecific F2 pedigree of Populus deltoides. A total of 1,107 testcross markers were obtained, and the mapping population consisted of 376 progeny. Among these markers, 597 were from the mother, and were assigned into 19 linkage groups, spanning a total genetic distance of 1,940.3 cM. The remaining 519 markers were from the father, and were also were mapped into 19 linkage groups, covering 2,496.3 cM. The genome coverage of both maps was estimated as greater than 99.9% at 20 cM per marker, and the numbers of linkage groups of both maps were in accordance with the 19 haploid chromosomes in Populus. Marker segregation distortion was observed in large contiguous blocks on some of the linkage groups. Subsequently, we mapped the segregation distortion loci in this mapping pedigree. Altogether, eight segregation distortion loci with significant logarithm of odds supports were detected. Segregation distortion indicated the uneven transmission of the alternate alleles from the mapping parents. The corresponding genome regions might contain deleterious genes or be associated with hybridization incompatibility. In addition to the detection of segregation distortion loci, the established genetic maps will serve as a basic resource for mapping genetic loci controlling traits of interest in future studies.

Identification and Characterization of Segregation Distortion Loci on Cotton Chromosome 18

“…With the development of molecular markers, SD has been widely reported in several plant species, particularly in interspecific crosses for genetic mapping, such as rice (Shanmugavadivel et al, 2013), wheat (Takumi et al, 2013; Adamski et al, 2014), chickpea (Ravikumar et al, 2013), Arabidopsis (Leppala et al, 2013), coffee (Gartner et al, 2013), soybean (Baumbach et al, 2012), potato (Manrique-Carpintero et al, 2016), Brassica rapa (Kitashiba et al, 2016), and Populus deltoids (Zhou et al, 2015). SD has previously been reported in interspecific populations of cotton (Guo et al, 2007; Blenda et al, 2012; Byers et al, 2012; Liang et al, 2012; Yu et al, 2013; Diouf et al, 2014; Zhang et al, 2014; Li et al, 2016), intraspecific populations of G. barbadense (Wang et al, 2013), intraspecific populations of G. hirsutum (Lin et al, 2009; Zhang et al, 2012, 2016; Ning et al, 2014), and intraspecific populations of G. arboreum (Li et al, 2007); however, the genetic mechanism of SD in cotton remains unclear.…”

Evaluation of SRAP markers for mapping of Pisum sativum L.

“…This strategy ensures the accuracy and a high coverage of the final map. Similar strategies were successfully used for linkage mapping in different species, e g., of olive (Khadari et al 2010), globe artichoke and cardoon (Martin et al 2013), lentil (Verma et al 2015), poplar (Zhou et al 2015), and wheat (Li et al 2015).…”

The first genetic linkage map forFraxinus pennsylvanicaand syntenic relationships with four related species

“…Segregation distortion (SD) is a general phenomenon in plants, but the percentage, degree and genetic effects may vary significantly across species (Dai et al 2016;Zhou et al 2015;Taylor and Ingvarsson 2003). In maize, it has been reported that 18 chromosome regions on 10 chromosomes were associated with SD (Lu, Romero-Severson, and Bernardo 2002) while 14 SD regions were reported for barley (Li et al 2010).…”