Genetic Manipulations of Oral Spirochete Treponema denticola

Kurniyati, Kurni; Li, Chunhao

doi:10.1007/978-1-0716-0939-2_2

Cited by 4 publications

(2 citation statements)

References 22 publications

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“…It contains some pathogenic species, such as Leptospira and Treponema pallidum, closely related to zoonotic leptospirosis and syphilis (72)(73)(74). Oral Treponema, the key pathogen related to periodontitis and gingivitis, has more than 60 species (75,76). Furthermore, periodontitis and gingivitis have a close association with T2DM.…”

Section: Discussionmentioning

confidence: 99%

Distribution characteristics of oral microbiota and its relationship with intestinal microbiota in patients with type 2 diabetes mellitus

et al. 2023

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IntroductionType 2 diabetes mellitus (T2DM) has a high incidence rate globally, increasing the burden of death, disability, and the economy worldwide. Previous studies have found that the compositions of oral and intestinal microbiota changed respectively in T2DM; whether the changes were associated or interacted between the two sites and whether there were some associations between T2DM and the ectopic colonization of oral microbiota in the gut still need to be identified.Research design and methodsWe performed a cross-sectional observational study; 183 diabetes and 74 controls were enrolled. We used high-throughput sequencing technology to detect the V3-V4 region of 16S rRNA in oral and stool samples. The Source Tracker method was used to identify the proportion of the intestinal microbiota that ectopic colonized from the oral cavity.ResultsThe oral marker bacteria of T2DM were found, such as Actinobacteria, Streptococcus, Rothia, and the intestinal marker bacteria were Bifidobacterium, Streptococcus, and Blautia at the genus level. Among them, Actinobacteria and Blautia played a vital role in different symbiotic relationships of oral and intestinal microbiota. The commonly distributed bacteria, such as Firmicutes, Bacteroidetes, and Actinobacteria, were found in both oral and intestine. Moreover, the relative abundance and composition of bacteria were different between the two sites. The glycine betaine degradation I pathway was the significantly up-regulated pathway in the oral and intestinal flora of T2DM. The main serum indexes related to oral and intestinal flora were inflammatory. The relative abundance of Proteobacteria in the intestine and the Spirochete in oral was positively correlated, and the correlation coefficient was the highest, was 0.240 (P<0.01). The proportion of ectopic colonization of oral flora in the gut of T2DM was 2.36%.ConclusionThe dysbacteriosis exited in the oral and intestine simultaneously, and there were differences and connections in the flora composition at the two sites in T2DM. Ectopic colonization of oral flora in the intestine might relate to T2DM. Further, clarifying the oral-gut-transmitting bacteria can provide an essential reference for diagnosing and treating T2DM in the future.

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Section: Discussionmentioning

confidence: 99%

Distribution characteristics of oral microbiota and its relationship with intestinal microbiota in patients with type 2 diabetes mellitus

et al. 2023

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“…The cloned flaB1 and flaB3 genes were then released by NotI and subcloned into B2UR‐ermB‐B2DR , generating two vectors, one with B2UR‐flaB1‐ermB‐B2DR and the other with B2UR‐flaB3‐ermB‐B2DR . To replace flaB2 with flaB1 or flaB3 , these two vectors were transformed into T. denticola wild‐type competent cells via electroporation as previously described (Kurniyati & Li, 2021). The obtained two mutants, ΔflaB2/B1 and ΔflaB2/B3 , were confirmed using PCR and immunoblotting.…”

Section: Methodsmentioning

confidence: 99%

Transcriptional and functional characterizations of multiple flagellin genes in spirochetes

Kurniyati

Chang

et al. 2022

Molecular Microbiology

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The flagellar filament is a helical propeller for bacterial locomotion. In external flagellates, the filaments are mostly homopolymers of a single flagellin protein. By contrast, the flagellar filaments of spirochetes are mostly heteropolymers of multiple flagellin proteins. This report seeks to investigate the role of multiple flagellin proteins using the oral spirochete Treponema denticola as a model. First, biochemical and genetic studies uncover that the flagellar filaments of T. denticola mainly comprise four proteins, FlaA, FlaB1, FlaB2, and FlaB3, in a defined stoichiometry. Second, transcriptional analyses reveal that the genes encoding these four proteins are regulated by two different transcriptional factors, sigma28 and sigma70. Third, loss‐of‐function studies demonstrate that each individual flagellin protein contributes to spirochete motility, but none of them is absolutely required. Last, we provide genetic and structural evidence that FlaA forms a “seam”‐like structure around the core and that deletion of individual flagellin protein alters the flagellar homeostasis. Collectively, these results demonstrate that T. denticola has evolved a unique mechanism to finely regulate its flagellar filament gene expression and assembly which renders the organelle with the right number, shape, strength, and structure for its distinct motility.

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Identification and Characterization of the Alternative σ ²⁸ Factor in Treponema denticola

et al. 2022

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Genetic Manipulations of Oral Spirochete Treponema denticola

Cited by 4 publications

References 22 publications

Distribution characteristics of oral microbiota and its relationship with intestinal microbiota in patients with type 2 diabetes mellitus

Distribution characteristics of oral microbiota and its relationship with intestinal microbiota in patients with type 2 diabetes mellitus

Transcriptional and functional characterizations of multiple flagellin genes in spirochetes

Identification and Characterization of the Alternative σ ²⁸ Factor in Treponema denticola

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Genetic Manipulations of Oral Spirochete Treponema denticola

Cited by 4 publications

References 22 publications

Distribution characteristics of oral microbiota and its relationship with intestinal microbiota in patients with type 2 diabetes mellitus

Distribution characteristics of oral microbiota and its relationship with intestinal microbiota in patients with type 2 diabetes mellitus

Transcriptional and functional characterizations of multiple flagellin genes in spirochetes

Identification and Characterization of the Alternative σ 28 Factor in Treponema denticola

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Identification and Characterization of the Alternative σ ²⁸ Factor in Treponema denticola