Identification and Characterization of the Alternative σ
            <sup>28</sup>
            Factor in Treponema denticola

Kurniyati, Kurni; Chang, Yifan; Liu, Jun; Li, Chunhao

doi:10.1128/jb.00248-22

Cited by 3 publications

(3 citation statements)

References 90 publications

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“…80 Since B. burgdorferi has no homologs of σ 28 (FliA), RpoD controls the flagellar and chemotaxis gene expression in this bacterium. 81 Furthermore, Treponema pallidum, the syphilis spirochaete, in addition to the housekeeping σ 70 factor (RpoD; TP0493) has four annotated alternative σ factors, i.e., σ 54 (RpoN; TP1011), σ A (TP1012), σ 28 (RpoF; TP0700), and one ECF σ E -type factor (RpoE; TP0092). 81 Unfortunately, their role is understudied.…”

Section: T a B L E 2 (Continued)mentioning

confidence: 99%

“…The RpoS‐dependent repression and activation of mammalian‐phase‐specific virulence factors (including key lipoprotein OspC) are critical for transmission from tick to mammalian host and establishing early stage of infection 80 . Since B. burgdorferi has no homologs of σ 28 (FliA), RpoD controls the flagellar and chemotaxis gene expression in this bacterium 81 . Furthermore, Treponema pallidum , the syphilis spirochaete, in addition to the housekeeping σ 70 factor (RpoD; TP0493) has four annotated alternative σ factors, i.e., σ 54 (RpoN; TP1011), σ A (TP1012), σ 28 (RpoF; TP0700), and one ECF σ E ‐type factor (RpoE; TP0092) 81 .…”

Section: Leptospira Interrogans σ Factorsmentioning

confidence: 99%

“…81 Furthermore, Treponema pallidum, the syphilis spirochaete, in addition to the housekeeping σ 70 factor (RpoD; TP0493) has four annotated alternative σ factors, i.e., σ 54 (RpoN; TP1011), σ A (TP1012), σ 28 (RpoF; TP0700), and one ECF σ E -type factor (RpoE; TP0092). 81 Unfortunately, their role is understudied. Still, TP0092 has been shown to be upregulated during experimental syphilis.…”

Section: T a B L E 2 (Continued)mentioning

confidence: 99%

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Sigma factors of RNA polymerase in the pathogenic spirochaete Leptospira interrogans, the causative agent of leptospirosis

Kędzierska‐Mieszkowska

2023

The FASEB Journal

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The aim of this review is to summarize the current knowledge on the role of σ factors in a highly invasive spirochaete Leptospira interrogans responsible for leptospirosis that affects many mammals, including humans. This disease has a significant impact on public health and the economy worldwide. In bacteria, σ factors are the key regulators of gene expression at the transcriptional level and therefore play an important role in bacterial adaptative response to different environmental stimuli. These factors form a holoenzyme with the RNA polymerase core enzyme and then direct it to specific promoters, which results in turning on selected genes. Most bacteria possess several different σ factors that enable them to maintain basal gene expression, as well as to regulate gene expression in response to specific environmental signals. Recent comparative genomics and in silico genome‐wide analyses have revealed that the L. interrogans genome, consisting of two circular chromosomes, encodes a total of 14 σ factors. Among them, there is one putative housekeeping σ70‐like factor, and three types of alternative σ factors, i.e., one σ54, one σ28 and 11 putative ECF (extracytoplasmic function) σE‐type factors. Here, characteristics of these putative σ factors and their possible role in the L. interrogans gene regulation (especially in this pathogen's adaptive response to various environmental conditions, an important determinant of leptospiral virulence), are presented.

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Section: T a B L E 2 (Continued)mentioning

confidence: 99%

Section: Leptospira Interrogans σ Factorsmentioning

confidence: 99%

Section: T a B L E 2 (Continued)mentioning

confidence: 99%

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Sigma factors of RNA polymerase in the pathogenic spirochaete Leptospira interrogans, the causative agent of leptospirosis

Kędzierska‐Mieszkowska

2023

The FASEB Journal

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Anti-σ ²⁸ Factor FlgM Regulates Flagellin Gene Expression and Flagellar Polarity of Treponema denticola

et al. 2023

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Genetic analysis of platelet-derived growth factor receptor-like gene (PDGFRL) polymorphism and melanin traits in Lanping black-boned sheep (Ovis aries)

Yue,

Peng,

Memon

et al. 2024

Arch. Anim. Breed.

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Abstract. In the intricate tapestry of Yunnan Province's biodiversity, the Lanping black-boned sheep (LPBB) emerges as a captivating enigma, distinguished by its profound melanin pigmentation adorning both its skin and its internal organs. Initially cataloged in the 1950s within the confines of Lanping County, this exceptional mammalian species presents a scarcity and uniqueness that extends beyond its geographic origins. Here, we collected 100 blood samples from Lanping black-boned sheep along with 50 samples each from Lanping normal sheep (LPN) and Huize normal sheep (HZN), all sourced from Yunnan Province. Our investigation focused on the association between the platelet-derived growth factor receptor-like gene (PDGFRL) polymorphism and the distinctive melanin characteristics observed in Lanping black-boned sheep. Utilizing UV–visible spectrophotometry, we assessed the melanin indexes present, such as tyrosinase activity and true melanin in the sheep blood, and the results demonstrated a significant elevation in melanin indexes for Lanping black-boned sheep compared to the control group (P<0.05). We also identified three synonymous mutation sites within a partial 1128 bp exon fragment of the gene-encoding PDGFRL (EX2-G408A, EX5-T184C, and EX5-G222T). Notably, Lanping black-boned sheep, harboring genotypes GG, TT, and GG at these specific sites, showcased a pronounced surge in tyrosinase activity, eumelanin / total melanin ratios, and plasma colorimetric values when contrasted with the control group (P<0.05). The discernment of GG, TT, and GG as the prevailing genotypes at their respective genetic loci in Lanping black-boned sheep heralds a breakthrough in our understanding of the genetic markers associated with black pigmentation. However, all three loci are silent mutations and do not alter the phenotypic changes. Whether they affect changes in melanin content through other metabolic pathways requires further study. In conclusion, the PDGFRL gene was silenced by mutations in our study and affected blood melanin levels. However, the gene did not undergo a missense mutation that altered the phenotypic changes, and the exact channel through which the changes in melanin content were affected needs to be further verified.

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Identification and Characterization of the Alternative σ ²⁸ Factor in Treponema denticola

Cited by 3 publications

References 90 publications

Sigma factors of RNA polymerase in the pathogenic spirochaete Leptospira interrogans, the causative agent of leptospirosis

Sigma factors of RNA polymerase in the pathogenic spirochaete Leptospira interrogans, the causative agent of leptospirosis

Anti-σ ²⁸ Factor FlgM Regulates Flagellin Gene Expression and Flagellar Polarity of Treponema denticola

Genetic analysis of platelet-derived growth factor receptor-like gene (PDGFRL) polymorphism and melanin traits in Lanping black-boned sheep (Ovis aries)

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Identification and Characterization of the Alternative σ 28 Factor in Treponema denticola

Cited by 3 publications

References 90 publications

Sigma factors of RNA polymerase in the pathogenic spirochaete Leptospira interrogans, the causative agent of leptospirosis

Sigma factors of RNA polymerase in the pathogenic spirochaete Leptospira interrogans, the causative agent of leptospirosis

Anti-σ 28 Factor FlgM Regulates Flagellin Gene Expression and Flagellar Polarity of Treponema denticola

Genetic analysis of platelet-derived growth factor receptor-like gene (PDGFRL) polymorphism and melanin traits in Lanping black-boned sheep (Ovis aries)

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Identification and Characterization of the Alternative σ ²⁸ Factor in Treponema denticola

Anti-σ ²⁸ Factor FlgM Regulates Flagellin Gene Expression and Flagellar Polarity of Treponema denticola